Detection and analysis of DNA repair using chemically-synthesized DNA sensors

• Project/Area Number: 21310142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: IWAI Shigenori 大阪大学, 大学院・基礎工学研究科, 教授 (10168544)
• Co-Investigator(Renkei-kenkyūsha): KURAOKA Isao 大阪大学, 大学院・基礎工学研究科, 准教授 (60335396)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: DNA損傷 / DNA修復 / オリゴヌクレオチド / 蛍光プローブ / 蛍プローブ

Research Abstract

DNA is always damaged by exogenous and endogenous factors, but all living organisms have repair systems for damaged DNA. We intended to develop sensors(or technically known as probes) to detect and analyze DNA repair reactions by fluorescence, and synthesized fluorescent probes that would be used for the reactions of base-excision-repair enzymes with oxidatively-damaged DNA. Using these probes, the substrate specificities of the repair enzymes were analyzed in vitro, and the cellular repair reactions were successfully visualized.

Research Products

• [Journal Article] Fluorescent probes for the analysis of DNA strand scission in base excision repair (2010)
  • Author(s): Naoyuki Matsumoto, Tatsuya Toga, Ryosuke Hayashi, Kaoru Sugasawa, Katsuo Katayanagi, Hiroshi Ide, IsaoKuraoka, Shigenori Iwai
  • Journal Title: Nucleic Acids Res. Volume: Vol.38
  • Peer Reviewed
• [Journal Article] DNA修復反応の蛍光による検出と解析 (2010)
  • Author(s): 岩井成憲
  • Journal Title: 生産と技術 Volume: Vol.62 Pages: 69-73
  • NAID: 40017156114
• [Journal Article] Fluorescent probes for the analysis of DNA strand scission in base excision repair (2010)
  • Author(s): Naoyuki Matsumoto, T.Toga, R.Hayashi, K.Sugasawa, K.Katayanagi, H.Ide, I.Kuraoka, S.Iwai
  • Journal Title: Nucleic Acids Research Volume: 38
  • Peer Reviewed
• [Journal Article] Fluorescence detection of the endonuclease IIIreaction using modified oligonucleotides (2009)
  • Author(s): Naoyuki Matsumoto, Ryosuke Hayashi, Masayuki Himoto, Isao Kuraoka, SayuriMorita, Fuki Hagiwara, KatsuoKatayanagi, Hiroshi Ide, Shigenori Iwai
  • Journal Title: Nucleic Acids Symp. Ser Volume: No.53 Pages: 213-214
• [Journal Article] Fluorescence detection of the endonuclease III reaction using modified oligonucleotides (2009)
  • Author(s): Naoyuki Matsumoto
  • Journal Title: Nucleic Acids Symposium Series No. 53 Pages: 213-214
• [Presentation] Fluorescent probes for the detection of base excision repair in cells (2011)
  • Author(s): 栂達也
  • Organizer: Sixth Cambridge Symposium on Nucleic Acids Chemistry and Biology
  • Place of Presentation: ケンブリッジ大学(イギリス)
  • Year and Date: 2011-09-04
• [Presentation] Fluorescence detection of the repair of radiation-induced DNA damage using synthetic oligonucleotides (2011)
  • Author(s): Shigenori Iwai
  • Organizer: Workshop of Research Laboratory for Quantum Beam Science on" RadiationEffects on DNA"
  • Place of Presentation: Osaka University, Suita, Osaka, Japan
  • Year and Date: 2011-07-26
• [Presentation] Fluorescence detection of the repair of radiation-induced DNA damage using synthetic oligonucleotides (2011)
  • Author(s): 岩井成憲
  • Organizer: Workshop of Research Laboratory for Quantum Beam Science on "Radiation Effects on DNA"
  • Place of Presentation: 大阪大学(招待講演)
  • Year and Date: 2011-07-26
• [Presentation] Fluorescent probes for the detection of base excision repair in cells (2011)
  • Author(s): Tatsuya Toga, Isao Kuraoka, Kaoru Sugasawa, Shigenori Iwai
  • Organizer: Sixth Cambridge Symposium on Nucleic Acids Chemistry and Biology
  • Place of Presentation: University of Cambridge, UK
• [Presentation] 蛍光による細胞の塩基除去修復能の検出 (2009)
  • Author(s): 岩井成憲
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-09
• [Presentation] Fluorescence detection of the endonuclease III reaction using modified oligonucleotides (2009)
  • Author(s): 岩井成憲
  • Organizer: The 6th International symposium on Nucleic Acids Chemistry
  • Place of Presentation: 高山市民文化会館(岐阜県)
  • Year and Date: 2009-09-29
• [Presentation] Fluorescence detection of base-excision-repair reactions using modified oligonucleotides (2009)
  • Author(s): 岩井成憲
  • Organizer: Nucleic Acids at the Chemistry-Biology Interface
  • Place of Presentation: University of Manchester, UK
  • Year and Date: 2009-09-08
• [Presentation] 蛍光による細胞の塩基除去修復能の検出 (2009)
  • Author(s): 栂達也, 松本直之, 菅澤薫, 倉岡功, 岩井成憲
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Fluorescence detection of the endonuclease III reaction using modified oligonucleotides (2009)
  • Author(s): Naoyuki Matsumoto, Ryosuke Hayashi, Masayuki Himoto, Isao Kuraoka, SayuriMorita, Fuki Hagiwara, Katsuo Katayanagi, Hiroshi Ide, Shigenori Iwai
  • Organizer: The 6th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Takayama, Gifu, Japan
• [Presentation] Fluorescence detectionof base-excision-repair reactions using modified oligonucleotides (2009)
  • Author(s): Naoyuki Matsumoto, Ryosuke Hayashi, Isao Kuraoka, Katsuo Katayanagi, Hiroshi Ide, Shigenori Iwai
  • Organizer: Nucleic Acids atthe Chemistry. Biology Interface
  • Place of Presentation: University of Manchester, UK
• [Remarks]
  • URL: http://www.bio.chem.es.osaka-u.ac.jp/