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Evaluation of nitrogen loading and its ecological consequences in the coastal marine ecosystem using compound-specific isotope analysis

Research Project

Project/Area Number 21370008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionThe University of Tokyo

Principal Investigator

MIYAJIMA Toshihiro  東京大学, 大気海洋研究所, 助教 (20311631)

Co-Investigator(Kenkyū-buntansha) NAGATA Toshi  東京大学, 大気海洋研究所, 教授 (40183892)
Co-Investigator(Renkei-kenkyūsha) TANAKA Yoshiyuki  独立行政法人海洋研究開発機構, むつ研究所, 研究員 (50396818)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords海洋生態 / 環境 / 窒素負荷 / アミノ酸 / 安定同位体比 / 大気降下物 / 共生系 / サンゴ礁 / 物質循環
Research Abstract

Compound-specific δ15N analysis has been employed to investigate ecological effects of anthropogenic nitrogen loading to the coastal marine ecosystems, particularly coral reefs. Spatial change in δ15N along environmental gradient of N loading was compared between the traditional bulk-δ15N and the amino acid δ15N measurements for both the host corals and the zooxanthellae. The mechanism for internal N exchange between the host coral and the zooxanthellae was investigated by comparing the amino acid δ15N pattern among corals incubated under different light and feeding conditions. To evaluate the detrital food chain using the amino acid δ15N, bacteria were incubated under several different nutritional conditions and compared for the amino acid δ15N pattern. We also evaluated the influence of atmospheric N deposition on coral reefs, which has been almost overlooked in previous works. The origin and the transport pathway were estimated using δ15N and δ18O of rainwater nitrate, δ18O and δ2H of meteoric water, and the backward trajectory analysis.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (23 results)

All 2013 2012 2011 2010 2009

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (11 results) Book (2 results)

  • [Journal Article] Bacterial decomposition of coral mucus as evaluated by long-term and quantitative observation2011

    • Author(s)
      Tanaka, Y., H. Ogawa, T. Miyajima
    • Journal Title

      Coral Reefs with referee

      Volume: 30 Issue: 2 Pages: 443-449

    • DOI

      10.1007/s00338-011-0729-3

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production and bacterial decomposition of dissolved organic matter in a fringing coral reef2011

    • Author(s)
      Tanaka Y, Ogawa H, Miyajima T
    • Journal Title

      Journal of Oceanography

      Volume: 67 Issue: 4 Pages: 427-437

    • DOI

      10.1007/s10872-011-0046-z

    • NAID

      10029524345

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of dissolved organic carbon and nitrogen in a coral reef2011

    • Author(s)
      Tanaka Y, Miyajima T, Watanabe A, Nadaoka K, Yamamoto T, Ogawa H
    • Journal Title

      Coral Reefs

      Volume: 30 Issue: 2 Pages: 533-541

    • DOI

      10.1007/s00338-011-0735-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of nutrient enrichment on the release of dissolved organic carbon and nitrogen by the scleractinian coral Montipora digitata2010

    • Author(s)
      Tanaka Y, Ogawa H, Miyajima T
    • Journal Title

      Coral Reefs

      Volume: 29 Pages: 675-682

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human impacts on large benthic foraminifers near a densely populated area of Majuro Atoll, Marshall Islands2010

    • Author(s)
      Osawa Y, Fujita K, Umezawa Y, Kayanne H, Ide Y, Nagaoka T, Miyajima T, Yamano H
    • Journal Title

      Marine Pollution Bulletin

      Volume: 60 Pages: 1279-1287

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autochthonous origin of semi-labile dissolved organic carbon in a large monomictic lake (Lake Biwa) : Carbon stable isotopic evidence2010

    • Author(s)
      Maki K, Kim C, Yoshimizu C, Tayasu I, Miyajima T, Nagata T
    • Journal Title

      Limnology

      Volume: 11 Pages: 143-153

    • NAID

      10027269204

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 栄養塩負荷に対する石灰化応答機構の解明2009

    • Author(s)
      田中泰章,宮島利宏,小川浩史,林原毅,小池勲夫
    • Journal Title

      月刊『地球』号外

      Volume: 59 Pages: 34-38

    • Related Report
      2011 Final Research Report
  • [Journal Article] Controlling factors of seasonal variation in the nitrogen isotope ratio of nitrate in a eutrophic coastal environment2009

    • Author(s)
      Sugimoto R, Kasai A, Miyajima T, Fujita K
    • Journal Title

      Estuarine, Coastal and Shelf Sciemce

      Volume: 85 Pages: 231-240

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Net release of dissolved organic matter by the scleractinian coral Acropora pulchra2009

    • Author(s)
      Tanaka Y, Miyajima T, Umezawa Y, Hayashibara T, Ogawa H, Koike I
    • Journal Title

      Journal of Experimental Marine Biology and Ecology

      Volume: 377 Pages: 101-106

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Longitudinal distribution of nitrate δ^<15>N and δ^<18>O in two contrasting tropical rivers : Implications for instream nitrogen cycling2009

    • Author(s)
      Miyajima T, Yoshimizu C, Tsuboi Y, Tanaka Y, Tayasu I, Nagata T, Koike I
    • Journal Title

      Biogeochemistry

      Volume: 95 Pages: 243-260

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] サンゴ礁の炭素循環研究への溶存無機炭素安定同位体比の応用2013

    • Author(s)
      宮島利宏
    • Organizer
      日本地球惑星科学連合2013年大会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] サンゴの内部素過程を考慮したストレス応答モデルの開発2012

    • Author(s)
      中村隆志
    • Organizer
      日本サンゴ礁学会第15回大会
    • Place of Presentation
      東京
    • Year and Date
      2012-11-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Seasonal and regional variability in nutrient inputs to sea surface by precipitation2012

    • Author(s)
      Miyajima T
    • Organizer
      1st Asia-Pacific Regional Symposium "Coastal Ecosystems Conservation and Adaptive Management"
    • Place of Presentation
      Quezon.
    • Year and Date
      2012-11-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Food habit analysis of commercially harvested jellyfish in the Perak river estuary, Bagan Datoh, Malaysia: a stable isotopic approach2012

    • Author(s)
      町田真通
    • Organizer
      2012 ASLO Aquatic Science Meeting
    • Place of Presentation
      大津市
    • Related Report
      2011 Annual Research Report
  • [Presentation] サンゴ礁域における大気降下物由来窒素負荷の状況とそのサンゴ礁生態系への潜在的影響2011

    • Author(s)
      宮島利宏:八重山諸島
    • Organizer
      日本地球惑星科学連合2011年大会
    • Place of Presentation
      千葉市
    • Year and Date
      2011-05-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 造礁サンゴ-褐虫藻間の共生的窒素交換-アミノ酸窒素安定同位体比による解析-2011

    • Author(s)
      藤井堯典
    • Organizer
      日本地球惑星科学連合2011年大会
    • Place of Presentation
      千葉市
    • Year and Date
      2011-05-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 造礁サンゴ-褐虫藻間の共生的窒素交換:アミノ酸窒素安定同位体比による解析2011

    • Author(s)
      宮島利宏
    • Organizer
      日本地球惑星科学連合2011年大会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 八重山諸島サンゴ礁域における大気降下物由来窒素負荷の状況とそのサンゴ礁生態系への潜在的影響2011

    • Author(s)
      宮島利宏
    • Organizer
      日本地球惑星科学連合2011年大会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Determi- nation of nitrogen sources for scleratinian corals by compound-specific isotope ratios of amino acids2010

    • Author(s)
      Fujii T
    • Organizer
      International Symposium on Isotope Ecology in Kyoto 2010
    • Place of Presentation
      京都市
    • Year and Date
      2010-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Determination of nitrogen sources for scleratinian corals by compound-specific isotoperatios of amino acids2010

    • Author(s)
      Fujii T, Miyajima T, Ogawa H, Tanaka Y, Watanabe A, Nadaoka K
    • Organizer
      International Symposium on Isotope Ecology 2010 in Kyoto
    • Place of Presentation
      京都
    • Year and Date
      2010-11-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Production and decomposition of dissolved organic matter in Shiraho coral reef (Ishigaki Island, Japan)2009

    • Author(s)
      田中泰章・宮島利宏・小川浩史
    • Organizer
      日本地球惑星科学連合2009年大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2009-05-16
    • Related Report
      2009 Annual Research Report
  • [Book] Stable isotope composition of nitrogen (δ^15N) as a tool for investigating nitrogen cycling in coral reef ecosystems.2010

    • Author(s)
      Miyajima T, Umezawa Y
    • Publisher
      In: Ohkouch Net al. (eds.) Earth, Life, and Isotopes, Kyoto University Press.
    • Related Report
      2011 Final Research Report
  • [Book] Stable isotope composition of nitrogen (δ^<15>N) as a tool for investigating nitrogen cycling in coral reef ecosystems, pp.197-222. In : Earth, Life, and Isotopes(Ohkouchi N et al (ed))2010

    • Author(s)
      Miyajima T, Umezawa Y
    • Total Pages
      415
    • Publisher
      Kyoto University Press
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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