|Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
During their evolution, vertebrate animals had invaded the land from water. Blood calcium needs a constant concentration to maintain normal life activities. However, a number of organs do exist for calcium regulation among vertebrate animals, depending on calcium concentration and/or its distribution in a given environment. Using mice, zebrafish and Polypterus, one of living species representing common ancestors of ray-finned fishes (actinopterygians), we have investigated evolutionary effects of changes in a number of Gcm genes and their regulatory pathways on development and function of each organ. We have carried out a construction of conditional knockout mice, enhancer analyses of Gcm genes involved in the formation of zebrafish ionocytes, developmental analyses of external gills in Polypterus and comparative genomics of Gcm genomes.