Molecular Mechanism of neuroectodermal determination by zinc-finger proteins
| Project/Area Number |
21370104
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SASAI Yoshiki 独立行政法人理化学研究所, 器官発生研究グループ, グループディレクター (20283616)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 神経発生 / 細胞分化 / 転写因子 / 外胚葉 / 中胚葉 / ES細胞 / Znフィンガー因子 / 多能性幹細胞 / Zfp521 / Zfp12 / XFDL156 / 神経分化 / P300 / アフリカツメガエル / Xenopus / 発生 / 分化 / アニマルキャップ / 脳形成 |
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| Research Abstract |
In this study, to understand the molecular mechanism underlying the neuroectodermal differentiation during early mammalian development, we analyzed the roles of two zinc-finger proteins that are involved in this process using in vitro differentiation system of pluripotent stem cells. We isolated mZfp12, a mammalian homolog of XFDL156, and showed that its overexpression in differentiating ES cells promotes neural differentiation and inhibits mesodermal differentiation. In addition, we iaolsted another zinc-finger protein Zfp521, and demonstrated its essential role in neuroectodermal differentiation from immature ectoderm.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Self-organizing optic-cup morphogenesis in three-dimensional culture.2011
Author(s)
Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T. and Sasai, Y.
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Journal Title
Nature
Volume: 472
Pages: 51-56
Related Report
Peer Reviewed
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[Journal Article] Intrinsic transition of ES cell differentiation into neural progenitors.2011
Author(s)
Kamiya, D., Banno, S., Sasai, N., Ohgushi, M., Watanabe, K., Kawada, M., Yakura, R., Jakt, L. M., Nishikawa, S. and Sasai. Y.
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Journal Title
Nature
Volume: 470
Pages: 503-509
Related Report
Peer Reviewed
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[Journal Article] In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction2009
Author(s)
Osakada, F., Jin, ZB., Hirami, Y., Ikeda, H., Danjyo, T., Watanabe, K., Sasai, Y., Takahashi, M.
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Journal Title
Journal of Cell Science
Volume: 122
Pages: 3169-79
Related Report
Peer Reviewed
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