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Elucidation of transposition mechanism a rice MITE Elucidation of a rice MITE mPing for development of high-efficiency transposon tagging system

Research Project

Project/Area Number 21380004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKyoto University

Principal Investigator

TSUKIYAMA Takuji (2010-2012)  京都大学, 大学院・農学研究科, 助教 (00423004)

谷坂 隆俊 (2009)  Kyoto University, 農学研究科, 教授 (80026591)

Co-Investigator(Kenkyū-buntansha) OKUMOTO Yutaka  京都大学, 大学院・農学研究科, 教授 (90152438)
TERAISHI Masayoshi  京都大学, 大学院・農学研究科, 講師 (80378819)
TSUKIYAMA Takuji  京都大学, 大学院・農学研究科, 助教 (00423004)
谷坂 隆俊  京都大学, 農学研究科, 名誉教授 (80026591)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsイネ / トランスポゾン / MITE / 転移機構 / 遺伝子発現 / ゲノム進化 / MITE / QTL / QTL / 遺伝子発現網
Research Abstract

Rice MITE, mPing is actively transposing in genome of cultivar ‘Gimbozu’ under natural conditions. In this study, we found that (1) mPing rendered adjacent genes stress inducible, (2) mPing transposition was genetically controlled specificin Gimbozu, and (3) a loss-of-function allele of Rurm1 was useful for acquiring the genetic diversity due to mPing transpositions. These results will greatly contribute to develop the high-efficiency transposon tagging system using mPing

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2013 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (16 results)

  • [Journal Article] Loss-of-function of a ubiquitin-related modifier promotes the mobilization of the active MITE mPing2013

    • Author(s)
      Tsukiyama T, Teramoto S, Yasuda K, Horibata A, Mori N, Okumoto Y, Teraishi M, Saito H, Onishi A, Tamura K, Tanisaka T
    • Journal Title

      Mol. Plant

      Volume: 6 Issue: 3 Pages: 790-801

    • DOI

      10.1093/mp/sst042

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Mobilization of the active transposon mPing in interspecific hybrid rice between Oryza sativa and O2012

    • Author(s)
      Yasuda K, Tsukiyama T, Karki S, Okumoto Y, Teraishi M, Saito H, Tanisaka T
    • Journal Title

      glabberima. Euphytica

      Volume: 74 Issue: 1 Pages: 430-432

    • DOI

      10.1007/s10681-012-0810-1

    • Related Report
      2012 Final Research Report
  • [Journal Article] イネユビキチン様タンパク質RURM1の標的タンパク質修飾機構の解析2012

    • Author(s)
      中田有美
    • Journal Title

      作物研究

      Volume: 57 Pages: 39-42

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] イネ活性型トランスポゾンmPingのための信頼性の高いトランスポゾンディスプレイ法2011

    • Author(s)
      寺本翔太、築山拓司、寺石政義、谷坂隆俊、奥本裕
    • Journal Title

      作物研究

      Volume: 56 Pages: 63-66

    • NAID

      110008711274

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM12010

    • Author(s)
      Takuji Tsukiyama
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 430-432

    • NAID

      10027551950

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wessler SR. Unexpected consequences of a sudden and massive transposon amplification on rice gene expression2009

    • Author(s)
      Naito K, Zhang F, Tsukiyama T, Saito H, Hancock CN, Richardson AO, Okumoto Y, Tanisaka T
    • Journal Title

      Nature

      Volume: 461 Issue: 7267 Pages: 1130-1134

    • DOI

      10.1038/nature08479

    • Related Report
      2012 Final Research Report
  • [Journal Article] Unexpected consequences of a sudden and massive transposon amplification on rice gene expression2009

    • Author(s)
      Ken Nito
    • Journal Title

      Nature 461

      Pages: 1130-1134

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of distribution and proliferation of mPing family transposons in a wild rice (Oryza rufipogon Griff.)2009

    • Author(s)
      Shanta Karki
    • Journal Title

      Breeding Science 59

      Pages: 297-307

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネトランスポゾン mPing の転移を活性化する染色体領域の同定2012

    • Author(s)
      吉田由梨
    • Organizer
      日本育種学会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 個体発生過程におけるイネトランスポゾン mPing の転移時機2012

    • Author(s)
      寺本翔太
    • Organizer
      日本育種学会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] イネトランスポゾンmPingの転移を活性化する染色体領域の同定2012

    • Author(s)
      吉田由梨
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 個体発生過程におけるイネトランスポゾンmPingの転移時機2012

    • Author(s)
      寺本翔太
    • Organizer
      日本育種学会第122回講演会
    • Place of Presentation
      京都産業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネユビキチン様タンパク質RURM1の機能喪失は細胞分裂の異常を引き起こす2012

    • Author(s)
      築山拓司
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネユビキチン様タンパク質RURM1 は細胞分裂の安定性に関与する2011

    • Author(s)
      角谷磨美
    • Organizer
      日本育種学会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] イネ活性型転移因子mPingを利用したストレス応答性遺伝子の発現改変を目指して2011

    • Author(s)
      安田加奈子、築山拓司、齋藤大樹、寺石政義、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会2011年秋季大会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] イネユビキチン様タンパク質RURM1は細胞分裂の安定性に関与する2011

    • Author(s)
      角谷磨美、築山拓司、那須田周平、寺石政義、谷坂隆俊、奥本裕
    • Organizer
      日本育種学会2011年秋季大会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] DNA メチル化がイネ非自律性転移因子 mPing の転移に与える影響2010

    • Author(s)
      森奈々子
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2010-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] mPingはなぜ転移するのか?-エピジェネッティクな制御機構から逸脱したイネ転移因子Pingの影響-2010

    • Author(s)
      門田有希、奥本裕
    • Organizer
      日本育種学会2010年秋季大会
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNAメチル化がイネ非自律性転移因子mPingの転移に与える影響2010

    • Author(s)
      森奈々子、築山拓司、寺石政義、奥本裕、谷坂隆俊
    • Organizer
      日本育種学会2010年秋季大会
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] mPing SCARマーカーを用いた新規出穂期突然変異遺伝子の同定2009

    • Author(s)
      浅見武人
    • Organizer
      日本育種学会2009年秋季大会
    • Place of Presentation
      北海道大学、北海道
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ転移因子の爆発的増殖がもたらす遺伝子発現網への影響2009

    • Author(s)
      内藤健
    • Organizer
      日本育種学会2009年秋季大会
    • Place of Presentation
      北海道大学、北海道
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネユビキチン様タンパク質RURM1の活性化酵素RUBA4の同定2009

    • Author(s)
      中田有美
    • Organizer
      日本育種学会2009年秋季大会
    • Place of Presentation
      北海道大学、北海道
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Mobilization of mPing and Pong transoposons by interspecific cross between Oryza sativa and O. glaberrima2009

    • Author(s)
      Shanta Karki
    • Organizer
      日本育種学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2009-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Mobilization of mPing and Pong transoposons by interspecific cross between Oryza sativa and O. glaberrima2009

    • Author(s)
      Shanta Karki
    • Organizer
      日本育種学会2009年春季大会
    • Place of Presentation
      つくば国際会議場、茨城
    • Year and Date
      2009-03-28
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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