Analysis of mechanism of ethylene biosynthesis by the post-translational regulation of ACC synthase with phosphorylation

• Project/Area Number: 21380026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Nagoya University
• Principal Investigator: MORI Hitoshi 名古屋大学, 生命農学研究科, 教授 (20220014)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 野菜 / エチレン / 翻訳後制御 / 代謝回転 / リン酸化 / 脱リン酸化 / ACC合成酵素 / protein phosphatase / rcn1 / CDPK / ホスファターゼ

Research Abstract

LeACS2 has two phosphorylated serine residues at C-terminal region by CDPK and MAPK. This phosphorylation should be stable for LeACS2 in cells. PP2A type of protein phosphatase dephosphorylates LeACS2. Some B subunits of PP2A recognize ACC synthase specifically.

Research Products

• [Journal Article] Isolation of the floral morph-related genes in heterostylous flax (Linum grandiflorum) : the genetic polymorphism and the transcriptional and post-transcriptional regulations of the S locus (2012)
  • Author(s): Koichiro Ushijima
  • Journal Title: Plant J. Volume: 69 Issue: 2 Pages: 317-331
  • DOI: 10.1111/j.1365-313x.2011.04792.x
  • Peer Reviewed
• [Journal Article] Chemical Visualization of an Attractant Peptide, LURE. (2011)
  • Author(s): Goto,H., et.al
  • Journal Title: Plant Cell Physiology Volume: 52 Pages: 49-58
  • Peer Reviewed
• [Journal Article] Turnover of LeACS2, a wound-inducible 1-amino-cyclopropane-1-carboxylic acid synthase in tomato, is regulated by phosphorylation/dephosphorylation. (2010)
  • Author(s): Kamiyoshihara, Y., Iwata, M., Fukaya, T., Tatsuki, M. and Mori, H.
  • Journal Title: Plant J. Volume: 64 Pages: 140-150
  • DOI: 10.1111/j.1365-313x.2010.04316.x
  • Year and Date: 2010-09-07
• [Journal Article] Turnover of LeACS2, a wound-inducible 1-aminocyclopropane-l-carboxylicacid synthase in tomato, is regulated by phosphorylation/dephosphorylation. (2010)
  • Author(s): Kamiyoshihara,Y., et.al
  • Journal Title: Plant Journal Volume: 64 Pages: 140-150
  • Peer Reviewed
• [Journal Article] The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water (2009)
  • Author(s): Hattori, Y., et al.
  • Journal Title: Nature 460 Pages: 1026-1030
  • Peer Reviewed
• [Journal Article] Auxin-cytokinin interactions in the control of shoot branching (2009)
  • Author(s): Sato-Shimizu, S., et al.
  • Journal Title: Plant Molecular Biology 69 Pages: 429-435
  • Peer Reviewed
• [Presentation] Analysis of the protein phosphatase involved in the post-translational regulatory mechanism of ACC synthase (2012)
  • Author(s): Hitoshi Mori
  • Organizer: Ethylene 9^<th> at Rotorua 2012
  • Place of Presentation: Rotorua Convention Centre (Rotorua, New Zealand)(招待講演)
  • Year and Date: 2012-03-20
• [Presentation] Analysis of the protein phosphatase involved in the post-translational regulatory mechanism of ACC synthase (2012)
  • Author(s): Mori, H.
  • Organizer: Ethylene
  • Place of Presentation: Rotorua, New Zealand
  • Year and Date: 2012-03-09
• [Presentation] トマトACC合成酵素LeACS2の安定化には2カ所のリン酸化部位が必要である (2010)
  • Author(s): 上吉原裕亮
  • Organizer: 植物化学調節学会 第45回大会
  • Place of Presentation: 神戸大学
  • Year and Date: 2010-11-02
• [Presentation] トマトACC合成酵素LeACS2の安定化には2カ所のリン酸化部位が必要である (2010)
  • Author(s): 森仁志
  • Organizer: 園芸学会 平成22年度秋季大会
  • Place of Presentation: 大分大学
  • Year and Date: 2010-09-19
• [Presentation] ACC合成酵素の脱リン酸化に関わるプロテインフォスファターゼの同定 (2010)
  • Author(s): 上吉原裕亮
  • Organizer: 園芸学会 平成22年度秋季大会
  • Place of Presentation: 大分大学
  • Year and Date: 2010-09-19
• [Presentation] The turnover of LeACS2, wound-inducible 1-aminocyclopropane-1-carboxylic acid synthase in tomato (2010)
  • Author(s): Kamiyoshihara, Y. and Mori, H.
  • Organizer: 20th International Conference on Plant Growth Substances
  • Place of Presentation: Tarragona, Spain
• [Presentation] トマトACC合成酵素LeACS2の安定化には2カ所のリン酸化部位が必要である (2010)
  • Author(s): 上吉原裕亮、森仁志
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 大分
• [Presentation] ACC合成酵素の脱リン酸化に関わるプロテインフォスファターゼの同定 (2010)
  • Author(s): 上吉原裕亮、森仁志
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 大分
• [Presentation] トマトACC合成酵素LeACS2の安定化には2カ所のリン酸化部位が必要である (2010)
  • Author(s): 森仁志、上吉原裕亮
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸
• [Presentation] Analysis of Protein Phosphatase Involved in the Post-translational Regulatory Mechanism of ACC Synthase (2009)
  • Author(s): Kamiyoshihara, Y., et al.
  • Organizer: The 8^<th> International Symposium on the Plant Hormone Ethylene
  • Place of Presentation: Cornell University, Ithaca, New York, USA
  • Year and Date: 2009-06-22
• [Book] "Ethylene." in Comprehensive Natural Products Chemistry II (2010)
  • Author(s): Mori, H. (edited by Mander, L., Lui, H.-W.)
  • Total Pages: 118
  • Publisher: Elsevier
• [Book] 新しい植物ホルモンの科学 第2版 (2010)
  • Author(s): 小柴共一・神谷勇治 編
  • Total Pages: 203
  • Publisher: 講談社
• [Book] Comprehensive Natural Products Chemistry II
  • Author(s): Mori, H.
  • Publisher: Elsevier : Oxford