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Analysis on the recognition mechanisms of molecular pattern of plant pathogenic fungi and signaling network

Research Project

Project/Area Number 21380031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionKyoto Prefectural University

Principal Investigator

KUBO Yasuyuki  京都府立大学, 大学院・生命環境科学研究科, 教授 (80183797)

Co-Investigator(Kenkyū-buntansha) MORI Masashi  石川県立大学, 生物資源工学研究所, 准教授 (00320911)
NISHIUCHI Takumi  金沢大学, 学際科学実験センター, 准教授 (20334790)
KOGA Hironori  石川県立大学, 生物資源環境学部, 教授 (60290079)
Co-Investigator(Renkei-kenkyūsha) YOSHIOKA Hirofumi  名古屋大学, 生命農学研究科, 准教授 (30240245)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords植物 / 微生物 / PAMPs / バイオテクノロジ- / 炭疽病菌 / いもち病菌 / マイクロアレイ / 植物免疫植物 / バイオテクノロジー / 植物免疫
Research Abstract

In this research project, to elucidate plant basal resistance mechanisms against plant pathogenic fungi, modification of cell surface structure of pathogen and its involvement of recognition and signaling network leading to plant defense response was analyzed. Especially, using rice-rice blast fungus and Arabidopsis thaliana. anthracnose fungus system, signaling network downstream of pathogen recognition was extensively analyzed based on genome based approach. Manipulation of pivotal transcription factor involving disease resistance enabled us to generate disease resistant transgenic rice plants.

Report

(5 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Products Report
  • Research Products

    (45 results)

All 2012 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (22 results) Book (3 results) Remarks (4 results)

  • [Journal Article] HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnaporthe oryzae2010

    • Author(s)
      Tanaka, S., Ichikawa, A., Yamada, K., Tsuji, G., Nishiuchi, T., Mori, M., Koga, H., Nishizawa, Y., O' Connell, R., and Kubo, Y.
    • Journal Title

      BMC Plant Biology

      Volume: 10 Pages: 288-288

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A yeast STE11 homologue CoMEKK1 is essential for pathogenesis-related morphogenesis in Colletotrichum orbiculare2010

    • Author(s)
      Sakaguchi, A., Tsuji, G., and Kubo, Y.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 23 Pages: 1563-1572

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxisome biogenesis factor PEX13 is required for appressorium-mediated plant infection by the anthracnose fungus, Colletotrichum orbiculare2010

    • Author(s)
      Fujihara, N., Sakaguchi, A., Tanaka, S., Fujii, S., Tsuji, G., Shiraishi, T., O' Connell, R., and Kubo, Y.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 23 Pages: 436-445

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Kelch repeat protein Cokel1p associates with microtubules and is involved in appressorium development in Colletotrichum orbiculare2010

    • Author(s)
      Sakaguchi, A., Miyaji, T., Tsuji, G., and Kubo, Y.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 23 Pages: 103-11

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of an efficient gene targeting system in Colletotrichum higginsianum using a non-homologous end-joining mutant and Agrobacterium tumefaciens-mediated gene transfer2010

    • Author(s)
      Ushimaru, T., Terada, H., Tsuboi, K., Kogou, Y., Sakaguchi, A., Tsuji G., and Kubo, Y.
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 284 Pages: 357-371

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pathogenesis and plant basal resistance in Colletotrichum orbiculare and Magnaporthe oryzae infection. In Genome-Enabled Integration of Research in Plant Pathogen Systems2010

    • Author(s)
      Kubo, Y., and Tanaka, S, Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K.
    • Journal Title

      APS Press

      Pages: 101-110

    • Related Report
      2011 Final Research Report
  • [Journal Article] Colletotrichum : species, ecology and interactions2010

    • Author(s)
      Damm, U., Baroncelli, R, . Cai, L., Kubo, Y., O' Connell, R., Weir, B., Yoshino K., and Cannon P.
    • Journal Title

      IMA Fungus

      Volume: 1 Pages: 161-165

    • Related Report
      2011 Final Research Report Products Report
    • Peer Reviewed
  • [Journal Article] HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnapor the oryzae2010

    • Author(s)
      S.Tanaka
    • Journal Title

      BMC Plant Biology

      Volume: 10 Pages: 288-288

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an efficient gene targeting system in Colletotrichum higginsianum using a non-homologous end-joining mutant and Agrobacterium tumefaciens-mediated gene transfer.2010

    • Author(s)
      T.Ushimaru
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 284 Pages: 357-371

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxisome biogenesis factor PEX13 is required for appressorium-Mediated plant infection by the anthracnose fungus, Colletotrichum orbiculare.2010

    • Author(s)
      Fujihara N.
    • Journal Title

      Molecular Plant-Microbe Interactions 23

      Pages: 436-445

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Kelch repeat protein Cokellp associates with microtubules and is involved in appressorium development in Colletotrichum orbiculare2010

    • Author(s)
      Sakaguchi A.
    • Journal Title

      Molecular Plant-Microbe Interactions 23

      Pages: 103-111

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxisome biogenesis factor PEX13 is required for appressorium-mediated plant infection by the anthracnose fungus, Colletotrichum orbiculare.2010

    • Author(s)
      N. Fujihara
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 23 Issue: 4 Pages: 436-445

    • DOI

      10.1094/mpmi-23-4-0436

    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway2009

    • Author(s)
      Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O' Connell, R., Tsuji, G., Tsuge, S., and Kubo, Y.
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 2517-2526

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RRS1 and RPS4 provide a dual resistance-gene system against fungal and bacterial pathogens2009

    • Author(s)
      Narusaka, M., Shirasu, K., Noutoshi, Y., Kubo, Y., Shiraishi, T., Iwabuchi M., and Narusaka, Y.
    • Journal Title

      Plant Journal

      Volume: 59 Pages: 672-683

    • NAID

      130006991284

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A dual resistance gene system prevents infection by three distinct pathogens2009

    • Author(s)
      Narusaka, M., Kubo, Y., Shiraishi, T., Iwabuchi, M., and Narusaka, Y.
    • Journal Title

      Signaling & Behavior

      Volume: 4 Pages: 954-955

    • NAID

      130006993828

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway2009

    • Author(s)
      Tanaka S.
    • Journal Title

      Plant Cell 21

      Pages: 2517-2526

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification and characterization of a novel pathogenicity-associated gene CoPRF1 in the anthracnose fungus Colletotrichum orbiculare2012

    • Author(s)
      Kaoru Tanaka and Yasuyuki Kubo
    • Organizer
      2nd Korea-Japan Joint Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of CoIRA1 of Colletotrichum orbiculare, an anthracnose disease fungus of cucumber, required for infection-related morphogenesis and pathogenicity2012

    • Author(s)
      Ken Harata and Yasuyuki Kubo
    • Organizer
      2nd Korea-Japan Joint Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Colletotrichum orbiculare CoBUB2, the homolog of Saccharomyces cerevisiae BUB2, is involved in morphogenesis and pathogenesis2012

    • Author(s)
      Fumi Fukada, Ayumu Sakaguchi, and Yasuyuki Kubo
    • Organizer
      2nd Korea-Japan Joint Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Cytological observation of mitotic chromosomes in Colletotricbum spp.and its implications for chromosome analysis, genome project and phylogenetics2011

    • Author(s)
      Y.Kubo
    • Organizer
      11^<th> European Fungal Genetics Conference
    • Place of Presentation
      ドイツ フィリップス大学
    • Year and Date
      2011-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional analysis of a novel pathogenicity-associated gene CoPRF1 in the anthracnose fungus Colletotrichum orbiculare2011

    • Author(s)
      Kaoru Tanaka and Yasuyuki Kubo
    • Organizer
      11th European conference on Fungal Generics
    • Place of Presentation
      Marburg, Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] Colletotrichum orbiculare Fam1p, a pex22 like peroxin unique in filamentous ascomycete, is required for appressorium mediated infection and associates with Woronin body2011

    • Author(s)
      Ayumu Sakaguchi, Naoki Fujihara1, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
    • Organizer
      Asian Mycological Congress
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2011 Final Research Report
  • [Presentation] Appressorium function in Colletotrichum orbiculare and prospect for genome based analysis2011

    • Author(s)
      Yasuyuki Kubo
    • Organizer
      14th Fungal Genetics and Biology Conference of the Microbiological Society of Korea
    • Place of Presentation
      Chungnam, Korea
    • Related Report
      2011 Final Research Report
  • [Presentation] Fam1p, a peroxin unique to filamentous ascomycete fungi, is required for pathogenesis and is transferred to Woronin bodies in Colletotrichum orbiculare2010

    • Author(s)
      Y.Kubo
    • Organizer
      第9回国際菌類学会(9th International Mycological Congress)
    • Place of Presentation
      イギリスエジンバラ国際会議場
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fam1p, a peroxin unique to filamentous ascomycete fungi, is required for pathogenesis and is transferred to Woronin bodies in Colletotrichum orbiculare2010

    • Author(s)
      Ayumu Sakaguchi, Naoki Fujihara, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
    • Organizer
      9th International Mycological Congress
    • Place of Presentation
      Edinburg, United Kindom
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel peroxin Fam1p of Colletotrichum orbiculare is essential for pathogenesis and associates with Woronin bodies2010

    • Author(s)
      Ayumu Sakaguchi, Naoki Fujihara, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
    • Organizer
      10th European conference on Fungal Generics
    • Place of Presentation
      Edinburg, United Kindom
    • Related Report
      2011 Final Research Report
  • [Presentation] Fungal cell surface PAMPs trigger plant basal resistance through activation of mitogen-activated protein kinase pathway2009

    • Author(s)
      Kubo Y.
    • Organizer
      14th International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      カナダ国 ケベック市
    • Year and Date
      2009-07-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Fungal cell surface PAMPs trigger plant basal resistance through activation of mitogen-activated protein kinase pathway2009

    • Author(s)
      Shigeyuki Tanaka, Nobuaki Ishihama, Hirofumi Yoshioka, Gento Tsuji, Seiji Tsuge and Yasuyuki Kubo
    • Organizer
      14th International Congress on Molecular Plant-Microbe Interactions Quebec
    • Place of Presentation
      Canada
    • Related Report
      2011 Final Research Report
  • [Presentation] ウリ類炭疽病菌RAS GTPase activated proteinCoIRA1は感染器官の分化や病原性に関与する

    • Author(s)
      原田賢・久保康之
    • Organizer
      平成24年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウリ類炭疽病菌の出芽酵母BUB2ホモログ遺伝子CoBUB2は形態形成および病原性に関与する

    • Author(s)
      深田史美・坂口歩・久保康之
    • Organizer
      平成24年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] イネいもち病菌感染初期過程におけるミトコンドリア凝集反応の観察

    • Author(s)
      望月進・齋藤憲一郎・久保康之・南栄一・西澤洋子
    • Organizer
      平成24年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] Genomic and transcriptomic analysis of the hemibiotrophic plant pathogen Colletotrichum orbiculare

    • Author(s)
      Gan, P., Narusaka, Y., Takano, Y., Kubo, Y., and Shirasu, K.
    • Organizer
      平成24年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] デュアル抵抗性蛋白質システムによる病原体認識機構の解明:RPS4とRRS1遺伝子の作物への導入

    • Author(s)
      鳴坂真理・白須賢・久保康之・白石友紀・畠山勝徳・平井正良・河本晃一・江面浩・高野義孝・岩渕雅樹・鳴坂義弘
    • Organizer
      平成23年度日本植物生理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] NAC-like transcription factors VOZは生物学的ストレスと非生物学的ストレスとの切り換え制御に関与する

    • Author(s)
      中井勇介・中平洋一・住田浩規・安居佑季子・河内孝之・高木優・光田展隆・久保康之・佐藤雅彦
    • Organizer
      日本植物学会平成23年度大会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウリ類炭疽病菌(Colletotrichum orbiculare) 104-T(MAFF240422)のゲノム解析

    • Author(s)
      高野義孝・多賀正節・鳴坂義弘・白須賢・久保康之
    • Organizer
      平成23年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウリ類炭疽病菌の核型と染色体の細胞学的解析

    • Author(s)
      多賀正節・田中薫・久保康之
    • Organizer
      平成23年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] デュアルR-遺伝子システムによる病原菌認識機構の解明

    • Author(s)
      鳴坂真理・白須賢・久保康之・豊田和弘・白石友紀・岩渕雅樹・鳴坂義弘
    • Organizer
      平成22年度日本植物病理学会大会
    • Related Report
      2011 Final Research Report
  • [Presentation] ウリ類炭疽病菌の侵入器官の形態形成と病原性

    • Author(s)
      久保康之
    • Organizer
      2011年第11回糸状菌分子生物学コンファレンス
    • Related Report
      2011 Final Research Report
  • [Book] Appressorium Function in Colletotrichum orbiculare and Prospect for Genome Based Analysis. In Morphogenesis and Pathogenicity in Fungi Series : Topics in Current Genetics2011

    • Author(s)
      Kubo, Y, Perez-Martin, Jose and Di Pietro, Antonio
    • Total Pages
      276
    • Related Report
      2011 Final Research Report
  • [Book] Genome-Enabled Integration of Research in Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J., Akimitsu, K.eds)2011

    • Author(s)
      Y.Kubo(分担執筆)
    • Total Pages
      272
    • Publisher
      APS Press
    • Related Report
      2010 Annual Research Report
  • [Book] 植物病理学2010

    • Author(s)
      久保康之(分担執筆)
    • Total Pages
      328
    • Publisher
      文永堂出版
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://ykubo.blog.eonet.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://ykubo.blog.eonet.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://eureka.kpu.ac.jp/~y_kubo/KuboHP/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://eureka.kpu.ac.jp/~y_kubo/KuboHP/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-09-09  

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