Proteins involved in transport and polymerization of monolignols

• Project/Area Number: 21380110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Kyoto University
• Principal Investigator: TAKABE Keiji 京都大学, 大学院・農学研究科, 教授 (70183449)
• Co-Investigator(Kenkyū-buntansha): YOSHINAGA Arata 京都大学, 大学院・農学研究科, 助教 (60273489) ; AWANO Tatsuya 京都大学, 大学院・農学研究科, 助教 (40324660)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: 分化中木部 / 細胞壁 / モノリグノール / 膜輸送 / コニフェリン / 木化 / シリンギルリグニン / T-DNA / 逆遺伝学 / シロイヌナズナ / リグニン前駆物質 / トランスポーター / 共発現解析 / 野生型 / 変異体 / ヒノキ / ポプラ / ミクロソーム / ATP / プロトン / 液胞

Research Abstract

Coniferin was actively transported into vacuole in the presence of ATP in early stage of secondary wall formation in poplar and Japanese cypress. Coniferin accumulated in vacuole gradually decreased in later stage of secondary wall formation. We collected the Arabidopsis seeds in which T-DNA was inserted to the candidate gene involved in monolignol transportation, and grew them for 10 to 12 weeks. Then we investigated the lignin distribution in interfasicular fibers of stalk. One of the transformants showed low syringyl lignin content in the secondary wall of interfasicular fibers.

Research Products

• [Journal Article] Occurrence of xylan and mannan polysaccharides and their spatial relationship with other cell wall components in differentiating compression wood tracheids of Cryptomeria japonica (2011)
  • Author(s): Jong Sik Kim, Awano T, Yoshinaga A, Takabe K
  • Journal Title: Planta Volume: 233(1) Issue: 4 Pages: 721-735
  • DOI: 10.1007/s00425-010-1333-4
  • Peer Reviewed
• [Journal Article] Temporal and spatial diversities of theimmunolabeling of mannan and xylanpolysaccharides in differentiatingearlywood ray cells and pits of Cryptomeria japonica (2011)
  • Author(s): Jong Sik Kim, Tatsuya Awano, Arata Yoshinaga and Keiji Takabe
  • Journal Title: Planta Volume: 233 Issue: 1 Pages: 109-122
  • DOI: 10.1007/s00425-010-1283-x
  • Peer Reviewed
• [Journal Article] Living wood fibers act as large-capacity "single-use" starch storage in black locust(Robinia pseudo acacia). (2011)
  • Author(s): Yamada Y, Awano T, Fujita M, Takabe K
  • Journal Title: Trees-Structure and Function Volume: 25(4) Issue: 4 Pages: 607-616
  • DOI: 10.1007/s00468-010-0537-3
  • Peer Reviewed
• [Journal Article] Occurrence of xylan and mannan polysaccharides and their spatial relationship with other cell wall components in differentiating (2011)
  • Author(s): Kim JS, Awano T, Yoshinaga A, Takabe K
  • Journal Title: Planta Volume: 233(4) Pages: 721-735
  • Peer Reviewed
• [Journal Article] Temporal and spatial diversities of the immunolabeling of mannan and xylan polysaccharides in differentiating earlywood ray cells and pits of Cryptomeria japonica (2011)
  • Author(s): Kim JS, Awano T, Yoshinaga A, Takabe K
  • Journal Title: Planta Volume: 233(1) Pages: 109-122
  • Peer Reviewed
• [Journal Article] Living wood fibers act as large-capacity "single-use" starch storage in black locust (Robinia pseudoacacia). Trees-Structure and Function (2011)
  • Author(s): Yamada Y, Awano T, Fujita M, Takabe K
  • Journal Title: Trees Volume: 25(4) Pages: 607-616
  • Peer Reviewed
• [Journal Article] Immunolocalization and structural variations of xylans in differentiating earlywood tracheid cell walls of Cryptomeria japonica (2010)
  • Author(s): Jong Sik Kim, Tatsuya Awano, Arata Yoshinaga and Keiji Takabe
  • Journal Title: Planta Volume: 232 Issue: 4 Pages: 817-824
  • DOI: 10.1007/s00425-010-1225-7
  • Peer Reviewed
• [Journal Article] Temporal and spatial immunolocalization of glucomannans in differentiating earlywood tracheid cell walls of Cryptomeria japonica (2010)
  • Author(s): Jong Sik Kim, Tatsuya Awano, Arata Yoshinaga and Keiji Takabe
  • Journal Title: Planta Volume: 232 Issue: 2 Pages: 545-554
  • DOI: 10.1007/s00425-010-1189-7
  • Peer Reviewed
• [Journal Article] Immunolocalization ofβ-1-4-galactan andits relationship with lignin distribution in developing compression wood of Cryptomeria japonica (2010)
  • Author(s): Jong Sik Kim, Tatsuya Awano, Arata Yoshinaga and Keiji Takabe
  • Journal Title: Planta Volume: 232 Issue: 1 Pages: 109-119
  • DOI: 10.1007/s00425-010-1152-7
  • Peer Reviewed
• [Journal Article] Cellular distribution of coniferin indifferentiating xylem of Chamaecyparisobtusa as revealed by Raman microscopy (2010)
  • Author(s): Yohei Morikawa, Arata Yoshinaga, Hiroshi Kamitakahara, Munehisa Wada and Keiji Takabe
  • Journal Title: Holzforschung Volume: 64 Issue: 1 Pages: 61-67
  • DOI: 10.1515/hf.2010.015
  • Peer Reviewed
• [Journal Article] Atsushi Furujyo and Kazuhiko Fukushima. Enzymatic saccharification of Eucalyptus bark using hydrothermal pre-treatment with carbon dioxide (2010)
  • Author(s): Yasuyuki Matsushita, KazuchikaYamauchi, Keiji Takabe, Tatsuya Awano, Arata Yoshinaga, Masashi Kato, Tetsuo Kobayashi, Takayuki Asada
  • Journal Title: Bioresource Technology Volume: 101 Issue: 13 Pages: 4936-4939
  • DOI: 10.1016/j.biortech.2009.09.041
  • Peer Reviewed
• [Journal Article] Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy (2010)
  • Author(s): Yohei Morikawa, Arata Yoshinaga, Hiroshi Kamitakahara, Munehisa Wada, Keiji Takabe
  • Journal Title: Holzforschung Volume: 64 Pages: 61-67
  • Peer Reviewed
• [Journal Article] mmunolocalization of β-1-4-galactan and its relationship with lignin distribution in developing compression wood of Cryptomeria japonica (2010)
  • Author(s): ong Sik Kim, Tatsuya Awano, Arata Yoshinaga, Keiji Takabe
  • Journal Title: Planta Volume: 232 Pages: 109-119
  • Peer Reviewed
• [Journal Article] Diffusion pathways for heartwood substances in Acacia mangium (2009)
  • Author(s): Chunhua Zhang, Hisashi Abe, Yuzou Sano, Takeshi Fujiwara, MinoruFujita and Keiji Takabe
  • Journal Title: IAWA Journal Volume: 30(1) Pages: 37-48
  • Peer Reviewed
• [Presentation] シロイヌナズナにおけるリグニン前駆物質輸送体候補LSL1の解析 (2012)
  • Author(s): 津山濯、高部圭司、尾形善之、小林優
  • Organizer: 第62回木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-16
• [Presentation] シロイヌナズナにおけるリグニン前駆物質輸送体候補LSL1の解析 (2012)
  • Author(s): 津山濯、高部圭司、尾形善之、小林優
  • Organizer: 日本木材学会
  • Place of Presentation: 北海道大学農学部
  • Year and Date: 2012-03-16
• [Presentation] シロイヌナズナT-DNA挿入変異体を用いたリグニン前駆物質輸送体の絞り込み (2011)
  • Author(s): 津山濯、高部圭司、尾形善之
  • Organizer: 第56回リグニン討論会
  • Place of Presentation: 鶴岡
  • Year and Date: 2011-09-15
• [Presentation] 木部形成研究の現状と今後 (2011)
  • Author(s): 高部圭司
  • Organizer: 日本木材学会
  • Place of Presentation: 京都大学(招待講演)
  • Year and Date: 2011-03-20
• [Presentation] シロイヌナズナ共発現解析によるリグニン前駆物質輸送タンパク質の絞り込み (2011)
  • Author(s): 津山濯、高部圭司、尾形善之
  • Organizer: 第61回に本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] シロイヌナズナ共発現解析によるリグニン前駆物質輸送タンパク質の絞り込み (2011)
  • Author(s): 津山濯、高部圭司、尾形善行
  • Organizer: 日本木材学会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-03-18
• [Presentation] ヒノキ分化中木部ミクロソーム膜画分へのコニフェリルアルコールの取込み (2010)
  • Author(s): 津山濯、高部圭司
  • Organizer: 第60回に本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-18
• [Presentation] ヒノキ分化中木部ミクロソーム膜画分へのコニフェリルアルコールの取込み (2010)
  • Author(s): 津山濯, 高部圭司
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎観光ホテル
  • Year and Date: 2010-03-18
• [Presentation] ATP dependent uptake of monolignols in microsomal membranes from several trees (2010)
  • Author(s): Taku TSUYAMA and Keiji TAKABE
  • Organizer: 12^<th> Cell Wall Meeting Porto
• [Presentation] ATP dependent uptake of monolignols in microsomal membranes from several trees (2009)
  • Author(s): 津山濯, 高部圭司
  • Organizer: 7^<th> Pacific Regional Wood Anatomy Conference
  • Place of Presentation: Legend hotel in Kuala Lunmur, Malaysia
  • Year and Date: 2009-08-03
• [Presentation] ATP dependent uptake of monolignols in microsomal membranes from several trees (2009)
  • Author(s): Taku TSUYAMA and Keiji TAKABE
  • Organizer: 7th Pacific Regional Wood Anatomy Conference Kuala Lumpur