| Project/Area Number |
21380112
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
YOSHIMIZU Mamoru 北海道大学, 大学院・水産科学研究院, 特任教授 (40122915)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Hisae 北海道大学, 大学院・水産科学研究院, 准教授 (50399995)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIZAWA Toyohiko 北海道大学, 大学院・水産科学研究院, 准教授 (10222184)
TAKAMI Ikuo 長崎県水産試験場, 対馬水産業普及指導センター, 所長 (50506178)
OSEKO Norihisa 水産総合研究センター増養殖研究所, 魚病診断・研修センター, センター長 (30221823)
OH Myung-Joo Nhonnam National University, Department of Aqualife Medicine, Professor
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 魚病 / ウイルス / ワクチン / ds-RNA / Poly(I:C) / インターフェロン / 免疫 |
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| Research Abstract |
In this study, we developed a novel fish-immunization with a synthetic double-stranded RNA [Poly(I:C)] and live pathogenic viruses. The fishes immunized with live pathogenic virus, followed by administration of Poly(I:C), became a transient, non-specific antiviral state, and those fish survive the initial immunization with live virus, thereby mounting a specific protective immune response against the injected pathogenic virus. Strong protective immune response was observed in rainbow trout, Japanese flounder and sevenband grouper by Poly(I:C)-immunization with IHNV, VHSV and NNV, respectively. It is conceivable that Poly(I:C)-immunization will eventually be applicable to a wide range of fish species as well as other viruses.
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