Microbial ecological research and bio-control of rearing water for fish seedlings

• Project/Area Number: 21380127
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Kinki University
• Principal Investigator: EGUCHI Mitsuru 近畿大学, 農学部, 教授 (40176764)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Akito 近畿大学, 農学部水産学科, 助教 (10548837) ; KATO Keitaro 近畿大学, 水産研究所, 准教授 (90330240) ; 中川 至純 近畿大学, 水産研究所, 助教 (70399111)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 種苗生産 / 微生物 / 微細藻類 / グリーンウォーター / 水作り / 善玉菌 / 微生物生態学 / ロゼオバクター / ビブリオ / ワムシ / 飼育水 / グリーン・ウォーター / ナノクロロプシス / プロバイオティック / 細菌群集 / 水作り(水づくり) / プロバイオティッ

Research Abstract

Green microalgae such as Nannochloropsis oculata are widely used for fish seedlings in aquaculture industry as Green Water. In this study, the abundance of Roseobacter clade is being focused, as some strains are known to produce inhibitory substances against fish pathogenic bacteria. In N. oculata culture, Roseobacter clade (RCA) were various and came to be abundant. Interestingly, the combination of Roseobacter clade and phytoplankton reinforced the killing effect of RCA against some V. anguillarum.

Research Products

• [Journal Article] Mixed cultures of the phytoplankton Nannochloropsis oculata and the marine bacterium Sulfitobacter sp (2012)
  • Author(s): Emilia Noor Sharifah, Mitsuru Eguchi
  • Journal Title: Mixed cultures of the phytoplankton Nannochloropsis oculata and the marine bacterium Sulfitobacter sp. RO3 inhibit the growth of virulent strains of the major fish pathogen Vibrio anguillarum Volume: 60 Pages: 39-45
  • Peer Reviewed
• [Journal Article] Benefits of live phytoplankton, Chlorella vulgaris, as a biocontrol agent against fish pathogen Vibrio anguillarum (2012)
  • Author(s): Emilia Noor Sharifah, Mitsuru Eguchi
  • Journal Title: Fisheries Science Volume: 78 Issue: 2 Pages: 367-373
  • DOI: 10.1007/s12562-011-0465-1
  • NAID: 10030124799
  • Peer Reviewed
• [Journal Article] Mixed cultures of phytoplankton Nannochloropsis oculata and the marine bacterium Sulfitobacter sp. RO3 inhibit the growth of virulent strains of the major fish pathogen Vibrio anguillarum. (2012)
  • Author(s): Sharifah Noor Emilia
  • Journal Title: Aquaculture Science Volume: 60 Pages: 39-45
  • Peer Reviewed
• [Journal Article] The phytoplankton Nannochloropsis oculata enhances the ability of Roseobacter clade bacteria to inhibit the growth of fish pathogen Vibrio anguillarum (2011)
  • Author(s): Emilia Noor Sharifah, Mitsuru Eguchi
  • Journal Title: PLoS ONE Volume: 6(10) Issue: 10 Pages: 1-8
  • DOI: 10.1371/journal.pone.0026756
  • Peer Reviewed
• [Journal Article] Possible role of microalga Nannochloropsis in controlling Vibrio species in fish larva rearing water (2011)
  • Author(s): Akito Taniguchi, Emilia Noor Sharifah, Mitsuru Eguchi
  • Journal Title: Aquaculture Science Volume: 59 Pages: 451-458
  • NAID: 10029848333
  • Peer Reviewed
• [Journal Article] Possible Role of Microalga Nannochloropsis in Controlling Vibrio Species in Fish Larva Rearing Water (2011)
  • Author(s): Taniguchi Akito
  • Journal Title: Aquaculture Science Volume: 59 Pages: 158-163
  • NAID: 10029848333
  • Peer Reviewed
• [Journal Article] 魚類飼育環境の微生物群集 (2009)
  • Author(s): 江口充
  • Journal Title: 日本水産学会誌 75 Pages: 707-707
• [Presentation] Roseobacter clade and Vibrio group bacterial diversity and abundances in rotifer culture fed with different types microalgae (2013)
  • Author(s): Emilia Noor Sharifah, Mitsuru Eguchi
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-27
• [Presentation] Potential chemical substances excreted by phytoplankton relating to the eradication of fish pathogen (2013)
  • Author(s): Mitsuru Eguchi
  • Organizer: International Annual Seminar on Marine Science & Aquaculture
  • Place of Presentation: サバ大学（マレーシア）
• [Presentation] Roseobacter clade and Vibrio group bacterial diversity and abundances in rotifer culture fed with different types microalgae (2013)
  • Author(s): 江口 充
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都）
• [Presentation] The existence of Roseobacter clade bacteria in concert with phytoplankton Nannochloropsis oculata enhances the inhibition effect against fish pathogen Vibrio anguillarum (2012)
  • Author(s): Emilia Noor Sharifah, Mitsuru Eguchi
  • Organizer: 第14回国際微生物生態学会(isme 14)
  • Place of Presentation: コペンハーゲン(デンマーク)
  • Year and Date: 2012-08-20
• [Presentation] Vibrio species bio-control by the phytoplankton by phytoplankton-bacteria in teraction in microcosm experiments (2012)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 2012 International Annual Seminar on Marine Science & Aquaculture
  • Place of Presentation: Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia
  • Year and Date: 2012-03-13
• [Presentation] The existence of Roseobacter clade bacteria in concert with phytoplankton Nannochloropsis oculata enhances the inhibition effect against fish pathogen Vibrio anguillarum (2012)
  • Author(s): Mitsuru Eguchi
  • Organizer: the14th International Symposium on Microbial Ecology
  • Place of Presentation: Bella Center(デンマーク)
• [Presentation] Promising anti-vibrio effect by the combination of phytoplankton and Roseobacter clade bacteria (2012)
  • Author(s): Mitsuru Eguchi
  • Organizer: The GCOE Program International Symposium of Kinki University 2012
  • Place of Presentation: 串本ロイヤルホテル（和歌山県）
• [Presentation] Cooperations between Roseobacter clade bacteria and phytoplankton Nannochloropsis oculata eradicate fish pathogen, Vibrio anguillarum (2011)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 平成23年度日本水産学会近畿支部後期例会
  • Place of Presentation: 大阪市立大学文化交流センター(大阪市)
  • Year and Date: 2011-11-26
• [Presentation] Vibrio species bio-control by the phytoplankton-bacteria interaction in a microcosm experiment (2010)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 第26回日本微生物生態学会大会
  • Place of Presentation: 筑波大学(茨城県)
  • Year and Date: 2010-11-25
• [Presentation] Promising relationship between microalgae, Nannochloropsis oculata and Roseobacter Glade bacteria in inhibiting different serotypes of fish path ogenic bacteria (2010)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-23
• [Presentation] Combination of Roseobacter clade affiliated bacteria and phytoplankton promised greater inhibition in fish pathogenic bacteria (2010)
  • Author(s): Emilia Noor Sharifah, Akito Taniguchi, Mitsuru Eguchi
  • Organizer: 第13回国際微生物生態学会(isme13)
  • Place of Presentation: シアトル(米国)
  • Year and Date: 2010-08-26
• [Presentation] Microalgae Nnnochloropsis controls Vibrio species growth in a rearing water microcosm (2010)
  • Author(s): 谷口亮人, Emilia Noor Sharifah, 江口充
  • Organizer: 第13回国際微生物生態学会(isme13)
  • Place of Presentation: シアトル(米国)
  • Year and Date: 2010-08-26
• [Presentation] Combination of Roseobacter clade affiliated bacteria and phytoplankton promised greater inhibition in fish pathogenic bacteria (2010)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 13^<th> International Symposium on Microbial Ecology
  • Place of Presentation: シアトル(USA)
  • Year and Date: 2010-08-24
• [Presentation] Microalgae Nnnochloropsis controls Vibrio species growth in a rearing water microcosm (2010)
  • Author(s): 谷口亮人
  • Organizer: 13^<th> International Symposium on Microbial Ecology
  • Place of Presentation: シアトル(USA)
  • Year and Date: 2010-08-24
• [Presentation] Promising bacteria and phytoplankton interaction in aquaculture industry (2010)
  • Author(s): Sharifah Noor Emilia
  • Organizer: Annual seminar on marine science & aquaculture
  • Place of Presentation: Kota Kinabalu, Sabah, Malaysia
  • Year and Date: 2010-03-11
• [Presentation] Can the addition of Nannochloropsis to rearing water control growth of Vibrio species? (2010)
  • Author(s): Akito Taniguchi
  • Organizer: Annual seminar on marine science & aquaculture
  • Place of Presentation: Kota Kinabalu, Sabah, Malaysia
  • Year and Date: 2010-03-11
• [Presentation] Effectiveness of phytoplankton and bacterial interaction for aquaculture (2010)
  • Author(s): Sharifah Noor Emilia
  • Organizer: Aquaculture 2010
  • Place of Presentation: San Diego, California
  • Year and Date: 2010-03-04
• [Presentation] Effectiveness of Nannochloropsis oculata and associated bacteria in inhibiting the growth of fish pathogenic bacteria (2009)
  • Author(s): Sharifah Noor Emilia
  • Organizer: 2009(平成21)年度日本水産学会秋季大会
  • Place of Presentation: 盛岡市
  • Year and Date: 2009-10-02
• [Remarks] 近畿大学農学部水産学科 水族環境学研究室
  • URL: http://kindai-suizoku.daa.jp/
• [Remarks] 近畿大学農学部
  • URL: http://nara-kindai.unv.jp/
• [Remarks] 近畿大学農学部水産学科 水族環境学研究室
  • URL: http://kindai-suizoku.daa.jp/
• [Remarks] 近畿大学農学部
  • URL: http://nara-kindai.unv.jp/
• [Remarks]
  • URL: http://kindai-suizoku.daa.jp/
• [Remarks]
  • URL: http://kindai-suizoku.daa.jp/
• [Remarks]
  • URL: http://kindai-suizoku.daa.jp/