Studies on how cytoskeleton-related molecules regulate neuronal migration, the brain formation as well as the maturation.

• Project/Area Number: 21390052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Fukui
• Principal Investigator: SATO Makoto 福井大学, 医学部, 教授 (10222019)
• Co-Investigator(Kenkyū-buntansha): YAGI Hideshi 兵庫医科大学, 医学部, 准教授 (10303372) ; XIE Min-jue 福井大学, 医学部, 助教 (40444210) ; IGUCHI Tokuichi 福井大学, 医学部, 特命助教 (60509305) ; IKEDA Hiroshi 福井大学, 工学研究科, 教授 (80377473)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 細胞分化 / 細胞形成 / 細胞骨格 / 解剖学 / 細胞・組織 / 神経科学 / 脳・神経 / 脂質 / アクチン / スパイン / 細胞移動

Research Abstract

It has been demonstrated that LL5beta and FILIP bind to filamin A, one of well-known actin-binding molecules, and exert their important activities over cell migration, especially radial migration that is essential for the neocortex development. In the present study, we further pursued the possible roles of LL5beta and FILIP in the adult brain and found that LL5beta plays an important role for spine development and that FILIP for synaptic transmission through modulating the morphology of spines and subcellular localization of glutamate receptors. In addition, we observed that some actin-binding molecules, other than filamin A, are involved in their activities.

Research Products

• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
  • Journal Title: Cerebral Cortex Volume: (in press) Issue: 6 Pages: 1410-1423
  • DOI: 10.1093/cercor/bhs123
  • Peer Reviewed
• [Journal Article] GPR98/Gpr98 Gene Is Involved in the Regulation of Human and Mouse Bone Mineral Density (2012)
  • Author(s): Urano, T., Shiraki, M, . Yagi, H., Ito, M., Sasaki, N., Sato, M., Ouchi, Y. and Inoue, S.
  • Journal Title: J. Clin. Endocrinol. Metab Volume: 97(4) Issue: 4 Pages: E565-E574
  • DOI: 10.1210/jc.2011-2393
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., Yagi, H., Wang, CC. and Sato, M.
  • Journal Title: PLoS ONE Volume: 7(3) Issue: 3 Pages: e33380-e33380
  • DOI: 10.1371/journal.pone.0033380
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., Asai, Y., Morii, M., Matsuki, M., Sato, M. and Nagao, T.
  • Journal Title: Toxicology Volume: 16 ; 295(1-3) Pages: 31-8
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., et al
  • Journal Title: PLoS ONE Volume: 7(3)
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., et al
  • Journal Title: Toxicology Volume: 295(1-3) Issue: 1-3 Pages: 31-8
  • DOI: 10.1016/j.tox.2012.02.013
  • Peer Reviewed
• [Journal Article] iPS細胞研究とその周辺技術、「特集1神経系におけるiPS細胞(iPS細胞の活用も含めた神経機能修復の現状と将来)」 (2011)
  • Author(s): 黒田一樹, 他
  • Journal Title: 脳21 Volume: 14(3)
• [Journal Article] LL5βdirects the translocation of Filamin A and SHIP2 to sites of PtdIns(3, 4, 5) P3 accumulation and PtdIns(3, 4, 5) P3 localization is mutually modified by co-recruited SHIP2 (2010)
  • Author(s): Takabayashi, T., Xie, M.-J., Takeuchi, S., Kawasaki, M., Yagi, H., Okamoto, M., Tariqur, R. M., Malik, F., Kuroda, K., Kubota, C., Fujieda, S., Nagano, T. and Sato, M.
  • Journal Title: J. Biol. Chem Volume: 285(21) Issue: 21 Pages: 16155-16165
  • DOI: 10.1074/jbc.m109.081901
  • Peer Reviewed
• [Journal Article] LL5β directs the translocation of Filamin A and SHIP2 to sites of PtdIns(3, 4, 5)P3 accumulation and PtdIns(3, 4, 5)P3 localization is mutually modified by corecruited SHIP2. (2010)
  • Author(s): Takabayashi, T, Xie, M.-J., Takeuchi, S.,Kawasaki, M,Yagi, H.,Okamoto, M.,Tariqur, R.M.,Malik, F.,Kuroda, K.,Kubota, C.,Fujieda, S.,Nagano, T.andSato, M.
  • Journal Title: J.Biol.Chem Volume: 285(21) Pages: 16155-16165
  • Peer Reviewed
• [Journal Article] LL5β directs the translocation of Filamin A and SHIP2 to sites of PtdIns(3,4,5)P3 accumulation and Ptdlns(3,4,5)P3 localization is mutually modified by co-recruited SHIP2. (2010)
  • Author(s): Takabayashi, T., Xie, M.-j., Takeuchi, S., Kawasaki, M., Yagi, H., Okamoto, M., Tariqur, R. M., Malik, F., Kuroda, K., Kubota, C., Fujieda, S., Nagano, T. and Sato, M.
  • Journal Title: J. Biol. Chem. Volume: 285 Issue: 21 Pages: 16155-16165
  • Peer Reviewed
• [Journal Article] Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly. (2009)
  • Author(s): Yamada M, Yoshida Y, Mori D, Takitoh T, Kengaku M, Umeshima H, Takao K, Miyakawa T, Sato M, Sorimachi H, Wynshaw-Boris A, Hirotsune S
  • Journal Title: Nat Med Volume: 15 Issue: 10 Pages: 1202-1207
  • DOI: 10.1038/nm.2023
  • Peer Reviewed
• [Journal Article] Deficiency of Vlgr1 resulted in deafness and susceptibility to audiogenic seizures while the degree of hearing impairment was not correlated with seizure severity in C57BL/6-and 129-backcrossed lines of Vlgr1 knockout mice (2009)
  • Author(s): Yagi, H., Noguchi, Y., Kitamura, K. and Sato, M.
  • Journal Title: Neurosci Lett Volume: 18 ; 461(2) Pages: 190-195
  • URL: http://dx.doi.org/10.1016/j.neulet
• [Journal Article] Deficiency of Vlgrl resulted in deafness and susceptibility to audiogenic seizures while the degree of hearing impairment was not correlated with seizure severity in C57BL/6- and 129-backcrossed lines of Vlgrl knockoutmice (2009)
  • Author(s): Yagi, H., Noguchi, Y., Kitamura, K., Sato, M.
  • Journal Title: Neurosci Lett. 18 ; 461(2) Pages: 190-195
  • Peer Reviewed
• [Journal Article] Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly (2009)
  • Author(s): Yamada, M., Yoshida, Y., Mori, D., Takitoh, T., Kengaku, M., Umeshima, H., Takao, K., Miyakawa, T., Sato, M., Sorimachi, H., Wynshaw-Boris, A., Hirotsune, S.
  • Journal Title: Nal.Med 15(10) Pages: 1202-1207
  • Peer Reviewed
• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration
  • Author(s): Xie, M.-J., et al
  • Journal Title: Cereb.Cortex Volume: (in press)
  • Peer Reviewed
• [Journal Article] LL5β directs the translocation of Filamin A and SHIP2 to sites of Ptdlns(3, 4, 5)P3 accumulation and Ptdlns(3, 4, 5)P3 localization is mutually modified by co-recruited SHIP2
  • Author(s): Takabayashi, T., Xie, M.-J., Takeuchi, S., Kawasaki, M., Yagi, H., Okamoto, M., Tariqur, R M., Malik, F., Kuroda, K., Kubota, C., Fujieda, S., Nagano, T., Sato, M
  • Journal Title: J.Biol.Chem. (in press)
  • Peer Reviewed
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開.シンポジウム(S19-3)「神経発生・形成における細胞骨格の意義」 (2012)
  • Author(s): 佐藤真
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開 (2012)
  • Author(s): 佐藤真
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] 大脳皮質における層構造異常とドパミン作動性神経投射との関連の解明 (2012)
  • Author(s): 竹田健彦, 他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] 神経細胞の棘状突起(スパイン)形成におけるFILIP関連分子の機能解析 (2012)
  • Author(s): 黒田一樹, 他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-27
• [Presentation] 皮質脊髄路の発達における軸策側枝形成因子の探索 (2012)
  • Author(s): 猪口徳一, 他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-26
• [Presentation] マイクロアレイ解析による大脳皮質長連合線維特異的遺伝子の同定 (2012)
  • Author(s): 岡雄一郎, 他
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-26
• [Presentation] こどものこころを考える(分子脳機能から発達障害まで) (2012)
  • Author(s): 佐藤真
  • Organizer: 大阪科学研究会「最先端医療科学学術シンポジウム-基礎研究から臨床応用まで-」
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2012-03-17
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling (2011)
  • Author(s): 佐藤真
  • Organizer: AACL-2011(Asian Aging Core for Longevity)長崎シンポジウムJapan-Korea Joint Conference on Brain Aging and Neurodegeneration
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-21
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling (2011)
  • Author(s): 佐藤真
  • Organizer: AACL-2011 (Asian Aging Core for Longevity)長崎シンポジウム
  • Place of Presentation: 長崎(招待講演)
  • Year and Date: 2011-11-21
• [Presentation] Dendritic growth cone activities, regulated by Abl kinase and Cdk5 via WAVE2-Abi2, are essential for completing multipolar-bipolar transition and starting glia-guided locomotion (2011)
  • Author(s): Xie, M.-J., et al.
  • Organizer: Society For Neuroscience 2011
  • Place of Presentation: ワシントン
  • Year and Date: 2011-11-15
• [Presentation] dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion. (2011)
  • Author(s): 佐藤真
  • Organizer: シンポジウム(S06-2)「神経形態制御から機能へ-スペーシャルセルバイオロジーの観点から」第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-27
• [Presentation] dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion (2011)
  • Author(s): 佐藤真
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-27
• [Presentation] Filip play a role in cortical development and spine morphology (2011)
  • Author(s): 八木秀司, 他
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-26
• [Presentation] 神経棘突起の形態調節におけるFILIPの役割 (2011)
  • Author(s): 八木秀司, 他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 中間前駆細胞の増殖・分化におけるヘッジホッグシグナルの役割 (2011)
  • Author(s): 駒田致和, 他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] 大脳皮質でのglia-guided locomotionの開始には、樹状突起の成長円錐が重要である (2011)
  • Author(s): 謝敏カク, 他
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] 福井大学での最近のデータ紹介 (2011)
  • Author(s): 佐藤真
  • Organizer: ORIGIN神経科学研究会夏のワークショップ
  • Place of Presentation: 兵庫
  • Year and Date: 2011-08-21
• [Presentation] Intermediate progenitorの増殖・分化におけるHedgehogシグナルの役割 (2011)
  • Author(s): 駒田致和, 他
  • Organizer: 第51回日本先天異常学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-22
• [Presentation] 大脳皮質形成期の法線方向移動における神経細胞の多極性から双極性への形態変化に成長円錐が重要である (2011)
  • Author(s): 謝敏カク, 黒田一樹, 八木秀司, 駒田致和, 猪口徳一, 佐藤真
  • Organizer: 第116回日本解剖学会・全国学術集会 第88回日本生理学会大会
  • Place of Presentation: 誌上開催
  • Year and Date: 2011-03-30
• [Presentation] FIHPの新たな側面:FILIP欠損による梨状葉神経細胞棘突起の形態変化 (2011)
  • Author(s): 八木秀司, 謝敏カク, 池田弘, 駒田致和, 猪口徳一, 黒田一樹, 岡部勝, 佐藤真
  • Organizer: 第116回日本解剖学会・全国学術集会 第88回日本生理学会大会
  • Place of Presentation: 誌上開催
  • Year and Date: 2011-03-30
• [Presentation] SNPアレイを用いた骨量との関連解析-ヒトゲノム、マウスモデル、ならびに発現解析を用いた骨粗鬆症疾患関連遺伝子の同定 (2010)
  • Author(s): 浦野友彦, 白木正孝,八木秀司, 佐藤真, 大内尉義, 井上聡
  • Organizer: 第12回日本骨粗鬆症学会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-10-21
• [Presentation] Large Scale Human SNP Analysis Revealed an Association of GPR98 Gene Polymorphisms with Bone Mineral Density in Postmenopausal Women and Gpr98 Deficient MiceDisplay Osteopenia (2010)
  • Author(s): Urano, T.,Yagi, H., Sato, M.,Ouchi, Y., Inoue, S.
  • Organizer: ASBMR 2010 Annual Meeting
  • Place of Presentation: Toronto(Canada)
  • Year and Date: 2010-10-18
• [Presentation] 福井大学での最近のデータ紹介 (2010)
  • Author(s): 佐藤真
  • Organizer: ORIGIN神経科学研究会夏のワークショップ
  • Place of Presentation: 奈良市
  • Year and Date: 2010-09-06
• [Presentation] Molecular analysis of amyloid beta precursor protein on the neuronal network formation using in utero electroporation gene transfer combined with inducible gene exression system. (2010)
  • Author(s): Iguchi, T.,Kuroda, K., Xie, M.-J.,Yagi, H., Sato, M.
  • Organizer: Neuro2010
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-04
• [Presentation] LL5β regulates morphology and maturation of dendritic spines. (2010)
  • Author(s): Xie, M-J., Iguchi, T.,Yagi, H., Shirao, T., Sato, M.
  • Organizer: Neuro2010
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-03
• [Presentation] 破綻から学ぶ脳形成・発達のしくみ:細胞分化・遊走から代謝、精神疾患まで (2010)
  • Author(s): 佐藤真
  • Organizer: 特別講演,日本薬学会北陸支部特別講演会
  • Place of Presentation: 富山
  • Year and Date: 2010-06-09
• [Presentation] 破綻から学ぶ脳形成・発達のしくみ:細胞分化・遊走から代謝、精神疾患まで (2010)
  • Author(s): 佐藤真
  • Organizer: 日本薬学会北陸支部特別講演会
  • Place of Presentation: 富山
  • Year and Date: 2010-06-09
• [Presentation] 大脳皮質において棘形成・発達と神経細胞遊走を司る新たな分子機構(S1-3)神経の発生・再生・移植研究会 (2010)
  • Author(s): 佐藤真
  • Organizer: 第25回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-22
• [Presentation] 大脳皮質において棘形成・発達と神経細胞遊走を司る新たな分子機構 (2010)
  • Author(s): 佐藤真
  • Organizer: 神経の発生・再生・移植研究会第25回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-22
• [Presentation] 胎仔期神経発生におけるアクチン繊維の可視化 (2010)
  • Author(s): 八木秀司, 謝敏カク, 猪口徳一, 黒田一樹, 佐藤真
  • Organizer: 第115回日本解剖学会・全国学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2010-03-30
• [Presentation] 大脳皮質形成関連因子の神経回路形成における機能解析～トランスポゾンによる染色体遺伝子導入と遺伝子発現誘導系を用いたアプローチ～ (2010)
  • Author(s): 猪口徳一, 黒田一樹, 謝敏カク, 八木秀司, 佐藤真
  • Organizer: 第115回日本解剖学会・全国学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2010-03-28
• [Presentation] Histone deacetylase SIRT1 modulates neuronal differentiation (2009)
  • Author(s): Hisahara, S., Tanno, M., Shimohama, S., Sato, M., Horio, Y.
  • Organizer: Society For Neuroscience 2009
  • Place of Presentation: シカゴ
  • Year and Date: 2009-10-20
• [Presentation] Phosphatidylinositol(3, 4, 5)-triphosphate associated factor LL5βregulates morphology and maturation of dendritic spines (2009)
  • Author(s): Xie, M-J., Yagi, H., Iguchi, T. and Sato, M
  • Organizer: ポスター大阪大学グローバルCOEポログラム"System Dynamics of Biological Fuction"
  • Place of Presentation: 淡路島
  • Year and Date: 2009-10-09
• [Presentation] Phosphatidylinositol (3, 4, 5)-triphosphate associated factor LL5βregulates morphology and maturation of dendritic spines (2009)
  • Author(s): Xie, M-J., Yajzi, H., Iguchi, T., Sato, M.
  • Organizer: 大阪大学ク"ローハ"ルCOEプログラム" System Dynamics of Biological Fuction"
  • Place of Presentation: 淡路島
  • Year and Date: 2009-10-08
• [Presentation] 大脳皮質形成の神経細胞移動においてAbi2はwAvE-Arp2/3を介して重要な役割を担う (2009)
  • Author(s): 謝敏カク, 八木秀司, 佐藤真
  • Organizer: 第52回日本神経化学会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-23
• [Book] iPS細胞研究とその周辺技術、「特集1神経系におけるiPS細胞(iPS細胞の活用も含めた神経機能修復の現状と将来) (2011)
  • Author(s): 黒田一樹,佐藤真
  • Publisher: 金芳堂
• [Remarks]
  • URL: http://www.med.u-fukui.ac.jp/KAIBOU2/