Arginine methylation in histone code

• Project/Area Number: 21390081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Shimane University
• Principal Investigator: URANO Takeshi 島根大学, 医学部, 教授 (70293701)
• Co-Investigator(Kenkyū-buntansha): SAKASHITA Gyosuk 島根大学, 医学部, 助教 (00397457) ; BAN Reiko 島根大学, 医学部, 助教 (40509105)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: ヒストン / 翻訳後修飾 / アルギニン残基 / メチル基 / 核内受容体コアクティベータ / がん関連転写制御因子 / メチル化 / 分裂期キナーゼ / NAC-1 / NCoA-3

Research Abstract

We identified a cancer-related transcriptional regulator protein NAC1 as a binding partner of histone-arginine methyltransferase PRMT4. The 450-kDa complex also contains nuclear receptor coactivator3NCoA-3. We revealed that PRMT4 regulates functions of hormone nuclear receptor and cancer-related transcription.

Research Products

• [Journal Article] Murakami Y and Nakayama J-I.: Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly (2012)
  • Author(s): Hayashi A, Ishida M, Kawaguchi R, Urano T
  • Journal Title: Proc. Natl Acad. Sci. USA Volume: 109 Pages: 6159-6164
• [Journal Article] Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells (2012)
  • Author(s): Inoko A, Matsuyama M, Goto H, Ohmuro-Matsuyama Y, Hayashi Y, Enomoto M, Ibi M, Urano T, Yonemura S, Kiyono T, Izawa I and Inagaki M.
  • Journal Title: J. Cell Biol. Volume: 197 Pages: 391-405
• [Journal Article] Rappsilber J and Allshire RC.: Raf1 is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification (2012)
  • Author(s): Buscaino A, White S, Houston D, Simmer F, Alves F, Diyora PT, Urano T, Bayne E
  • Journal Title: PLoS Genet. Volume: 8
• [Journal Article] pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen (2012)
  • Author(s): Matsumoto Y, Zhang Q, Akita K, Nakada H, Hamamura K, Tokuda N, Tsuchida A, Matsubara T, Hori T, Okajima T, Furukawa K, Urano T and Furukawa K
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 419 Pages: 7-13
• [Journal Article] DNA : RNA hybrid formation mediates RNAi-directed heterochromatin formation (2012)
  • Author(s): Nakama M, Kawakami K, Kajitani T, Urano T and Murakami Y
  • Journal Title: Genes Cells Volume: 17 Pages: 218-233
• [Journal Article] pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen (2012)
  • Author(s): Matsumoto Y.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 419 Pages: 7-13
  • Peer Reviewed
• [Journal Article] Raf1 is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification (2012)
  • Author(s): Buscaino A.
  • Journal Title: PLoS Genet Volume: 8
  • Peer Reviewed
• [Journal Article] Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly (2012)
  • Author(s): Hayashi A.
  • Journal Title: Proc.Natl Acad.Sci.USA Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis (2011)
  • Author(s): Ban R, Nishida T and Urano T
  • Journal Title: Genes Cells Volume: 16 Pages: 652-669
• [Journal Article] The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles (2011)
  • Author(s): Ma N, Matsunaga S, Morimoto A, Sakashita G, Urano T, Uchiyama S and Fukui K
  • Journal Title: J. Cell Sci Volume: 124 Pages: 394-404
  • NAID: 120006994997
• [Journal Article] The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. (2011)
  • Author(s): Ma N.
  • Journal Title: J.Cell Sci. Volume: 124 Pages: 394-404
  • Peer Reviewed
• [Journal Article] Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast (2010)
  • Author(s): Simmer F, Buscaino A, Kos-Braun IC, Kagansky A, Boukaba A, Urano T, Kerr AR and Allshire RC
  • Journal Title: EMBO Rep Volume: 11 Pages: 112-118
• [Journal Article] Preparation, characterization and properties of novel covalently surface-functionalized zinc oxide nanoparticles (2010)
  • Author(s): Sato M, Harada H, Morito S, Fujita Y, Shimosaki S, Urano T and Nakamura M
  • Journal Title: Appl. Surf. Sci Volume: 256 Pages: 4497-4501
• [Journal Article] Hairpin RNA induces secondary small interfering RNA synthesis and silencinq in trans in fission yeast. (2010)
  • Author(s): Simmer F.
  • Journal Title: EMBO Rep. 11 Pages: 112-118
  • Peer Reviewed
• [Journal Article] Preparation, characterization and properties of novel covalently surface-functionalized zinc oxide nanoparticles. (2010)
  • Author(s): Sato M.
  • Journal Title: Appl.Surf.Sci. 256 Pages: 2297-4501
  • Peer Reviewed
• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases (2009)
  • Author(s): Ban R, Matsuzaki H, Akashi T, Sakashita G, Taniguchi H, Park S-Y, Tanaka H, Furukawa K and Urano T
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 28367-28381
• [Journal Article] CRMP-2 directly binds to cytoplasmic dynein and interferes with its activity (2009)
  • Author(s): Arimura N, Hattori A, Kimura T, Nakamuta SI, Funahashi Y, Hirotsune S, Furuta K, Urano T, Toyoshima YY, Kaibuchi K
  • Journal Title: J Neurochem Volume: 111 Pages: 380-390
• [Journal Article] Synthetic heterochromatin bypasses RNAi and centromeric repeats to establish functional centromeres (2009)
  • Author(s): Kagansky A, Folco HD, Almeida R, Pidoux AL, Boukaba A, Simmer F, Urano T, Hamilton GL and Allshire RC
  • Journal Title: Science Volume: 324 Pages: 1716-1719
• [Journal Article] Mitotic regulation of the stability of microtubule plus-end tracking protein EB3 by ubiquitin ligase SIAH-1 and Aurora mitotic kinases. (2009)
  • Author(s): Ban R.
  • Journal Title: J.Biol.Chem. 284 Pages: 28367-28381
  • Peer Reviewed
• [Journal Article] CRMP-2 directly binds to cytoplasmic dynein and interferes with its activity. (2009)
  • Author(s): Arimura N.
  • Journal Title: J.Neurochem. 111 Pages: 380-390
  • Peer Reviewed
• [Journal Article] Synthetic heterochromatin bypasses RNAi and centromeric repeats to establish functional centromeres. (2009)
  • Author(s): Kagansky A.
  • Journal Title: Science 324 Pages: 1716-1719
  • Peer Reviewed
• [Presentation] Biological and spatial regulation of transcriptional regulatory protein NAC-1 (2012)
  • Author(s): Urano T
  • Organizer: Tufts University
  • Place of Presentation: Boston, USA
  • Year and Date: 2012-03-13
• [Presentation] Biological and spatial regulation of transcriptional regulatory protein NAC-1 (2012)
  • Author(s): Urano T.
  • Organizer: Tufts University
  • Place of Presentation: Boston, USA(招待講演)
  • Year and Date: 2012-03-13
• [Presentation] SUMO化による分裂期キナーゼAuroraの制御 (2010)
  • Author(s): 浦野健
  • Organizer: 第8回SUMO研究会
  • Place of Presentation: 筑波大学(筑波)
  • Year and Date: 2010-11-26
• [Presentation] SUMO化による分裂期キナーゼAuroraの制御 (2010)
  • Author(s): 浦野健
  • Organizer: 第8回SUMO研究会
  • Place of Presentation: 筑波
  • Year and Date: 2010-11-26
• [Presentation] The spatial regulation of a transcriptional regulator NAC-1. (2010)
  • Author(s): Urano T
  • Organizer: The 1st International Worksh op on Structural Epigenomics
  • Place of Presentation: Riken Yokohama Institute Koryuto Hall(Y okohama)
  • Year and Date: 2010-10-01
• [Presentation] The spatial regulation of a transcriptional regulator NAC-1. (2010)
  • Author(s): Urano T.
  • Organizer: The 1st International Workshop on Structural Epigenomics
  • Place of Presentation: Yokohama(招待講演)
  • Year and Date: 2010-10-01
• [Presentation] Biological and domain analysis of transcriptional regulatory prote in NAC-1 (2010)
  • Author(s): Urano T.
  • Organizer: The 6^<th> International Forum on Oxidative Stress and Aging
  • Place of Presentation: Nagoya Congress Center(Nagoya)
  • Year and Date: 2010-09-07
• [Presentation] Biological and domain analysis of transcriptional regulatory protein NAC-1. (2010)
  • Author(s): Urano T.
  • Organizer: The 6^<th> International Forum on Oxidative Stress and Aging
  • Place of Presentation: Nagoya(招待講演)
  • Year and Date: 2010-09-07
• [Presentation] 転写制御因子NAC1の細胞内動態と機能解析 (2010)
  • Author(s): 浦野健
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: ラフォーレ修善寺(静岡)
  • Year and Date: 2010-05-28
• [Presentation] 転写制御因子NAC1の細胞内動態と機能解析 (2010)
  • Author(s): 浦野健
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 静岡
  • Year and Date: 2010-05-28
• [Presentation] ひらめき☆ときめきサイエンス体験講座 実施報告 (2010)
  • Author(s): 成相裕子
  • Organizer: 平成21年度実験・実習技術研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2010-03-05
• [Presentation] 転写制御因子NAC1の機能ドメイン解析と細胞内動態 (2010)
  • Author(s): 加藤太陽
  • Organizer: 第27回染色体ワークショッフ
  • Place of Presentation: 静岡
  • Year and Date: 2010-01-21
• [Presentation] 分裂酵母ヘテロクロマチン由来non-coding RNAはDNA:RNA hybridを介してクロマチンに結合し、ヘテロクロマチン形成に関与する (2010)
  • Author(s): 仲間美奈
  • Organizer: 第27回染色体ワークショッフ
  • Place of Presentation: 静岡
  • Year and Date: 2010-01-20
• [Presentation] 分裂期キナーゼAurora-BのSUMO化の解析 (2009)
  • Author(s): 伴玲子
  • Organizer: 第7回SUMO研究会
  • Place of Presentation: 神戸
  • Year and Date: 2009-11-21
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html
• [Patent(Industrial Property Rights)] 新規モノクローナル抗体、及びそのタグ抗体としての利用 (2012)
  • Inventor(s): 浦野健、辰巳香澄、関根浄治
  • Industrial Property Rights Holder: 島根大学
  • Filing Date: 2012-03-13
  • Overseas
• [Patent(Industrial Property Rights)] 新規モノクローナル抗体、及びそのタグ抗体としての利用 (2012)
  • Inventor(s): 浦野健、辰巳香澄、関根浄治
  • Industrial Property Rights Holder: 島根大学
  • Filing Date: 2012-03-13
  • Overseas
• [Patent(Industrial Property Rights)] 物質相互作用をリアルタイムに可視化する技術 (2011)
  • Inventor(s): 岡崎宏亮、成相裕子、加藤太陽、浦野健
  • Industrial Property Rights Holder: 島根大学
  • Industrial Property Number: 2011-076772
  • Filing Date: 2011-03-30
• [Patent(Industrial Property Rights)] 新規モノクローナル抗体、及びそのタグ抗体としての利用 (2011)
  • Inventor(s): 浦野健、辰巳香澄
  • Industrial Property Rights Holder: 島根大学
  • Industrial Property Number: 2011-055953
  • Filing Date: 2011-03-14