Structural and functional analysis of the mechanism for HIV-1 evolution/ adaptation
| Project/Area Number |
21390141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ADACHI Akio 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90127043)
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| Co-Investigator(Kenkyū-buntansha) |
NOMAGUCHI Masako 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (80452647)
MIYAKE Ariko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (20548622)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | ウイルス / HIV-1 / 適応進化 / ゲノム解析 / アクセサリー蛋白質 / 適応変異 / 種特異性 |
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| Research Abstract |
Using the viral adaptation in cells and genetic engineering in vitro, we have successfully generated macaque-tropic HIV-1(HIV-1mt) that evades the anti-viral effect of all known anti-HIV-1 factors present in macaque cells(CypA, TRIM5., APOBEC3 and Tetherin). By improving the infection system of HIV-1mt and macaque monkeys, it would be possible to analyze experimentally the battle between HIV-1 and hosts. This would lead to the accomplishment of in vivo evaluation system for HIV-1 otherwise impossible.
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Report
(4 results)
Research Products
(79 results)
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[Journal Article] Geographical, genetic and functional diversity of antiretroviral host factor TRIMCyp in cynomolgus macaque(Macaca fascicularis)2012
Author(s)
Saito, A., Kono, K., Nomaguchi, M., Yasutomi, Y., Adachi, A., Shioda, T., Akari, H., and Nakayama, E. E
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Journal Title
Journal of General Virology
Volume: 93
Pages: 594-602
Related Report
Peer Reviewed
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[Journal Article] The identification of a small molecule compound that reduces HIV-1 Nef-mediated viral infectivity enhancement2011
Author(s)
Chutiwitoonchai, N., Hiyoshi, M., Mwimanzi, P., Ueno, T., Adachi, A., Ode, H., Sato, H., Fackler, O. T., Okada, S., and Suzu, S
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Journal Title
PLoS One
Volume: 6
Issue: 11
Pages: e27696-e27696
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] HIV-Nef impairs multiple T cell functions in antigen-specific immune response in mice2011
Author(s)
Fujii, H., Ato, M., Takahashi, Y., Otake, K., Hashimoto, S., Kaji, T., Tsunetsugu-Yokota, Y., Fujita, M., Adachi, A., Nakayama, T., Taniguchi, M., Koyasu, S., and Takemori, T
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Journal Title
International Immunology
Volume: 23
Pages: 433-441
Related Report
Peer Reviewed
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[Journal Article] Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications2011
Author(s)
Saito, A., Nomaguchi, M., Iijima, S., Kuroishi, A., Yoshida, T., Lee, Y.-J., Hayakawa, T., Kono, K., Nakayama, E. E., Shioda, T., Yasutomi, Y., Adachi, A., Matano, T., and Akari, H
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Journal Title
Microbes and Infection
Volume: 13
Pages: 58-64
NAID
Related Report
Peer Reviewed
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[Journal Article] Different interaction between HIV-1 Vif and its cellular target proteins APOBEC3G/ APOBEC3F2010
Author(s)
Nagao, T., Yamashita, T., Miyake, A., Uchiyama, T., Nomaguchi, M., and Adachi, A
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Journal Title
Journal of Medical Investigation
Volume: 57
Pages: 89-94
NAID
Related Report
Peer Reviewed
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[Journal Article] Modification of a loop sequence between a-helices 6 and 7 of virus capsid(CA) protein in a human immunodeficiency virus type 1(HIV-1) derivative that has simian immunodeficiency virus(SIVmac239) vif and CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells2009
Author(s)
Kuroishi, A., Saito, A., Shingai, Y., Shioda, T., Nomaguchi, M., Adachi, A., Akari, H., and Nakayama, E. E
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Journal Title
Retrovirology
Volume: 6
Pages: 70-70
Related Report
Peer Reviewed
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[Journal Article] Amino acid alterations in Gag that confer the ability to grow in simian cells on HIV-1 are located at a narrow CA region2009
Author(s)
Nagao, T., Hatcho, K., Doi, N., Fujiwara, S., Adachi, A., and Nomaguchi, M
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Journal Title
Journal of Medical Investigation
Volume: 56
Pages: 21-25
NAID
Related Report
Peer Reviewed
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[Presentation] Genotypic variation of cynomolgus monkey trim5alpha determines the susceptibility to monkey-tropic HIV-1 infection2011
Author(s)
Saito A, Nomaguchi M, Kono K, Nakayama, EE, Shioda T, Yoshida T, Yasutomi Y, Matano T, Adachi A, Akari H
Organizer
XV International Congress of Virology
Place of Presentation
Sapporo Convention Center(Sapporo, Japan)
Year and Date
2011-09-15
Related Report
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[Presentation] Analysis of growth adaptive mutations in HIV-1 genome identifies a pol-integrase region that enhances virion production in a cell-independent and codon triplet-dependent manner2010
Author(s)
Miyake, A., Doi, N., Fujiwara, S., Adachi, A., and Nomaguchi, M
Organizer
The 10th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai International Conference Center(Awaji, Japan)
Year and Date
2010-09-09
Related Report
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[Presentation] Modification of a loop betweenα-helices 6 and 7 of virus capsid protein improves human immunodeficiency virus type 1 replication in cynomolgus monkey cells2009
Author(s)
Kuroishi, A., Saito, A., Shingai, Y., Shioda, T., Nomaguchi, M., Adachi, A., Akari, H., and Nakayama, E. E
Organizer
The 9th Awaji International Forum on Infection and Immunity
Place of Presentation
Awaji Yumebutai International Conference Center(Awaji, Japan)
Year and Date
2009-09-10
Related Report
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