Elucidation of roles of essential trace metals in higher nervous activity

• Project/Area Number: 21390185
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Hokkaido University
• Principal Investigator: SAITO Takeshi 北海道大学, 大学院・保健科学研究院, 教授 (40153811)
• Co-Investigator(Kenkyū-buntansha): 蔵崎 正明 北海道大学, 大学院・地球環境科学研究院, 助教 (80161727) ; 細川 敏幸 北海道大学, 高等教育推進機構, 教授 (00157025) ; 藤田 博美 北海道大学, 大学院・医学研究科, 教授 (60142931) ; 村山 俊彦 千葉大学, 大学院・薬学研究院, 教授 (90174317) ; 田中 俊逸 北海道大学, 大学院・地球環境科学研究院, 教授 (30142194)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 微量金属 / 金属イオン膜輸送蛋白 / 中枢神経系 / 神経伝達物質 / 老化 / メタロシャペロン / 脳機能

Research Abstract

The aim of this study is to elucidate roles of trace elements, metal transporters and metallochaperone in the central nervous system. Our results indicate that reduction of Cu transporter Ctr1 but not MT induction is the primary cause of the inhibition of Cu absorption by excessive intake of Zn. Declines of syntheses of catecholamines and zinc concentrations were observed in the cerebral cortex of SAMP10 with aging. Cu-polyphenol induces apoptosis via CCS-XIAP degradation induced by incorporated Cu in PC12 cells

Research Products

• [Journal Article] Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction (2012)
  • Author(s): Sato S., Mukai Y and Saito T.
  • Journal Title: Journal of Nutritional Biochemistry Volume: (in press)
  • Peer Reviewed
• [Journal Article] Diethyl phthalate enhances apoptosis induced by serum deprivation in PC12 cells (2012)
  • Author(s): Sun Y, Takahashi K, Saito T, Hosokawa T, Kurasaki M
  • Journal Title: Basic & Clinical Pharmacology & Toxicology Volume: (in press)
  • NAID: 120005303469
  • Peer Reviewed
• [Journal Article] Study on toxicity of boron detected in Toyohira river water Using PC12 Cells (2012)
  • Author(s): Kitamura M, Sikder Md. T, Saito T, Kurasaki M
  • Journal Title: Biomed Res Trace Elements Volume: 23(1) Pages: 24-32
  • NAID: 10030756128
  • Peer Reviewed
• [Journal Article] Dysfunction of neurogenic VIP-mediated relaxation in mouse distal colon with dextran sulfate sodium-induced colitis (2012)
  • Author(s): Kato E., Yamane S., Nomura R., Matsumoto K, Tashima K., Horie S., Saito T., Fujino H., Murayama T.
  • Journal Title: Pharmacological Research Volume: 65 Pages: 204-212
  • Peer Reviewed
• [Journal Article] Diethyl phthalate enhances apoptosis induced by serum deprivation in PC12 cells (2012)
  • Author(s): Sun Y
  • Journal Title: Basic & Clinical Pharmacology & Toxicology
  • Peer Reviewed
• [Journal Article] Bisphenol-A suppresses neurite extension due to inhibition of phosphorylation of mitogen-activated protein kinase in PC12 cells (2011)
  • Author(s): Seki S, Aoki M, Hosokawa T, Saito T, Masuma R, Komori M, Kurasaki M.
  • Journal Title: Chemico-Biological Interactions Volume: 194 Pages: 23-30
  • NAID: 120003483496
  • Peer Reviewed
• [Journal Article] Activation of ceramidase and ceramide kinase by vanadate via a tyrosine kinase-mediated pathway (2010)
  • Author(s): Tada E., Toyomura K., Nakamura H., Sasaki H., Saito T., Kaneko M., Okuma Y., Murayama T.
  • Journal Title: Journal of Pharmacological Sciences Volume: 114(4) Pages: 420-432
  • NAID: 10029891066
  • Peer Reviewed
• [Journal Article] Activation of ceramidase and ceramide kinase by vanadate via a tyrosine kinase-mediated pathway. (2010)
  • Author(s): Toda E, et al.
  • Journal Title: Journal of Pharmacological Sciences Volume: 114 Pages: 420-432
  • Peer Reviewed
• [Journal Article] Inositol1,4,5-trisphosphate signaling maintains the activity of glutamate uptake in Bergmann glia. (2010)
  • Author(s): Mashimo M, et al.
  • Journal Title: European Journal Neuroscience Volume: 32 Pages: 1668-1677
  • Peer Reviewed
• [Journal Article] Comparison of general water quality of rivers in Indonesia and Japan (2009)
  • Author(s): Kido, M., Yustiawati, Syawal, MS, Sulastri, Hosokawa, T., Tanaka, S., Saito, T., Iwakuma, T., Kurasaki, M.
  • Journal Title: Environmental Monitoring and Assessment Volume: 156 Pages: 317-329
  • Peer Reviewed
• [Journal Article] Effects of synthetic sphingosine-1-phosphate analogs on cytosolic phospholipase A2alpha-independent release of arachidonic acid and cell toxicity in L929 fibrosarcoma cells : the structure-activity relationship. (2009)
  • Author(s): Shimizu Masaya
  • Journal Title: Journal of Pharmacological Sciences 109 Pages: 431-443
  • Peer Reviewed
• [Journal Article] Comparison of general water quality of rivers in Indonesia and Japan. (2009)
  • Author(s): Kido, Machiko.
  • Journal Title: Environmental Monitoring and Assessment 156 Pages: 87-98
  • Peer Reviewed
• [Presentation] 加齢及び老化に伴う大脳皮質内での抗酸化酵素Prxの回路異常 (2012)
  • Author(s): 宮島美貴
  • Organizer: 第82回日本衛生学会
  • Place of Presentation: 京都大学,京都
  • Year and Date: 2012-03-25
• [Presentation] 銅取り込みによるCCS-XIAP分解を介した銅混合クルクミンのアポトーシス増強作用 (2012)
  • Author(s): 西村亮
  • Organizer: 第82回日本衛生学会
  • Place of Presentation: 京都大学,京都
  • Year and Date: 2012-03-25
• [Presentation] 加齢及び老化に伴う大脳皮質内での抗酸化酵素Prxの回路異常 (2012)
  • Author(s): 宮島美貴
  • Organizer: 第82回日本衛生学会
  • Place of Presentation: 京都大学吉田キャンパス(京都)
  • Year and Date: 2012-03-25
• [Presentation] 銅取り込みによるCCS-XIAP分解を介した銅混合クルクミンのアポトーシス増強作用 (2012)
  • Author(s): 西村亮
  • Organizer: 第82回日本衛生学会
  • Place of Presentation: 京都大学吉田キャンパス(京都)
  • Year and Date: 2012-03-25
• [Presentation] Curcumin and copper binding curcumin induced apoptosis in PC12 cells via mitochondrial pathways (2011)
  • Author(s): Nishimura R
  • Organizer: 3rd International Symposium on Trace Elements & Health
  • Place of Presentation: Cultura CajaMurcia (Spain)
  • Year and Date: 2011-05-26
• [Presentation] Elucidation of the role of zinc, zinc-enzyem and tetrahydrobiopterin in the alteration of chatecholamine metabolism in the cerebral cortex with aging in senescence-accelerated mouse (2011)
  • Author(s): Miyajima
  • Organizer: 3rd International Symposium on Trace Elements & Health
  • Place of Presentation: Cultura CajaMurcia (Spain)
  • Year and Date: 2011-05-26
• [Presentation] PC12細胞におけるクルクミンの細胞死誘導に対する銅の制御機構 (2011)
  • Author(s): 西村亮
  • Organizer: 第81回日本衛生学会
  • Place of Presentation: 昭和大学,東京
  • Year and Date: 2011-03-26
• [Presentation] 老化促進モデルマウスSAMP10の脳内BH4代謝とその合成酵素の変動 (2011)
  • Author(s): 宮島美貴
  • Organizer: 第81回日本衛生学会
  • Place of Presentation: 昭和大学,東京
  • Year and Date: 2011-03-26
• [Presentation] 母親ラットに対する低蛋白食が出生後のラットの成長に及ぼす影響について (2011)
  • Author(s): 宮島美貴
  • Organizer: 第81回日本衛生学会
  • Place of Presentation: 昭和大学,東京
  • Year and Date: 2011-03-26
• [Presentation] Curcumin and copper binding curcumin induced apoptosis in PC12 cells via mitochondrial pathways. (2011)
  • Author(s): Nishimura R et al.
  • Organizer: 3rd International Symposium on Trace Elements & Health
  • Place of Presentation: Murcia, Spain
• [Presentation] Elucidation of the role of zinc, zinc-enzyme and tetrahydrobiopterin in the alteration of chatecholamine metabolism in the cerebral cortex with aging in senescence-accelerated mouse (2011)
  • Author(s): Miyajima M et al.
  • Organizer: 3rd International Symposium on Trace Elements & Health
  • Place of Presentation: Murcia, Spain
• [Presentation] Mechanism of toxicity on human vascular endothelial cells by humic acid obtained from tropical peatland (2011)
  • Author(s): Kihara, Y et al.
  • Organizer: 3rd International Symposium on Trace Elements & Health
  • Place of Presentation: Murcia, Spain
• [Presentation] Effects of humic acid on eNOS activities in human umbilical vein endothelial cells (2011)
  • Author(s): Tanaka M. et al.
  • Organizer: 3rd International Workshop on Wild Fire and Carbon Management in Peat-Forest in Indonesia
  • Place of Presentation: Palangka Raya, Indonesia
• [Presentation] 老化促進モデルマウスSAMP10の脳内テトラハイドロバイオプテリン(BH4)代謝の変動 (2010)
  • Author(s): 齋藤健
  • Organizer: 第21回日本微量元素学会
  • Place of Presentation: 京都大学,京都
  • Year and Date: 2010-07-03
• [Presentation] 老化促進モデルマウスSAMP10の脳内テトラハイドロバイオプテリン(BH4)代謝の変動 (2010)
  • Author(s): 齋藤健
  • Organizer: 第21回日本微量元素学会
  • Place of Presentation: 京都 招待講演
  • Year and Date: 2010-07-03
• [Presentation] 各種ウコン中のクルクミノイドおよび微量金属含有量の分析と関連性の検討 (2010)
  • Author(s): 西村亮
  • Organizer: 第80回日本衛生学会
  • Place of Presentation: 仙台国際センター,仙台
  • Year and Date: 2010-05-10
• [Presentation] 各種ウコン中のクルクミノイドおよび微量金属含有量の分析と関連性の検討 (2010)
  • Author(s): 西村亮
  • Organizer: 第80回日本衛生学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-10
• [Presentation] 老化促進モデルマウス(SAMP10)の加齢に伴う脳内カテコールアミン代謝の変動機構の解明 (2009)
  • Author(s): 蓑島萠未
  • Organizer: 第20回日本微量元素学会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-03
• [Presentation] 亜鉛摂取による腸管での銅の吸収阻害機構 (2009)
  • Author(s): 齋藤健
  • Organizer: 第13回ウィルソン病研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-09
• [Presentation] 老化促進モデルマウス(SAMP10)の加齢に伴う脳内カテコールアミン代謝の変動機構の解明 (2009)
  • Author(s): 蓑島萠未
  • Organizer: 第20回日本微量元素学会
  • Place of Presentation: 京王プラザホテル,東京
• [Presentation] クルクミン・銅および同時添加により引き起こされる細胞死とその機構 (2009)
  • Author(s): 佐藤睦将
  • Organizer: 第20回日本微量元素学会
  • Place of Presentation: 京王プラザホテル,東京
• [Book] 公衆衛生学社会・環境と健康第3版 (2011)
  • Author(s): 齋藤健,他
  • Publisher: 講談社サイエンティフィック
• [Book] 公衆衛生学社会・環境と健康第3版 (2011)
  • Author(s): 斎藤健
  • Publisher: 講談社サイエンティフィツク