| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Research Abstract |
We have previously proposed a model of hematopoiesis in which myeloid potential is retained along with development towards T, B and erythroid lineages. In this research project, we aimed to validate this model. Validation of myeloid-B progenitors in fetal hematopoiesis by a single cell analysis was completed(to be submitted). Validation of myeloid-T progenitor stage was achieved by fixing myeloid-T progenitors using a developmental arrest-inducing culture system(Ikawa et al, Science, 2010). Fate map approaches using genetic marking mouse have not yet completed, but a useful line(Flt3-ER-Cre mouse) has been successfully made, and preliminary experiment has provided some promising data. Collectively, the project has been very successful.
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