Microarray analysis to detect genes predicting organotropism of lung cancer metastasis.

• Project/Area Number: 21390395
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: YOSHIMASU Tatsuya 和歌山県立医科大学, 医学部, 講師 (60316099)
• Co-Investigator(Kenkyū-buntansha): OURA Shoji 和歌山県立医科大学, 医学部, 准教授 (10194060) ; HIRAI Yoshimitsu 和歌山県立医科大学, 医学部, 学内助教 (10508013)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 肺癌 / 臓器特異性 / マイクロアレイ / 遠隔転移 / seed and soil theory / 組織培養法 / 副腎転移 / sccd and soil thcory

Research Abstract

Lung cancer easily metastasizes to multiple organs, such as bone, lung, brain, liver, and adrenal gland. We applied microarray analysis to find the gene expression influencing organotropism of lung cancer metastasis. Human lung adenocarcinoma cell lines were used. Cell were cultured on the fresh tissue slice of athymic mouse. After that, proliferated cancer cells were collected from the surface of organ slice and cultured in the flask again. Then the conditioned cells from each organ were obtained. Microarray analysis was applied to these cells including original cells in order to detect specific gene alteration in each organ. Gene expressions of conditioned cells obtained from each organ were compared with original cells. Organ specific alterations were checked using unpaired t-test. We detected 76 genes as adrenal metastasis specific alterations, 22 genes with lung, 212 with liver, 25 with kidney, 141 with bone, and 27 with muscle.

Research Products

• [Presentation] Calbindin-D28k expression is correlated with the organotropism of lung cancer adrenal metastasis (2013)
  • Author(s): Yoshimasu T, Oura S, Kokawa Y, Kawago M, Hirai Y, Ohashi T, Nakamura R, Nishiguchi H, Matsutani M, Honda M, Okamura Y
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Sydney
• [Presentation] 肺癌の副腎転移におけるcalbindin-D28kの関与 (2013)
  • Author(s): 吉増達也, 尾浦正二, 粉川庸三, 川後光正, 平井慶充, 大橋拓矢, 中村理恵, 西口春香, 松谷雅子, 本田麻里子, 岡村吉隆
  • Organizer: 第66回日本胸部外科学会定期学術集会
  • Place of Presentation: 仙台
• [Presentation] Calbindin-D28k expression is correlated with the organotropism of lung cancer adrenal metastasis. (2013)
  • Author(s): Yoshimasu T, Oura S, Kokawa Y, Kawago M, Hirai Y, Ohawshi T, Nakamura R, Nishiguchi H, Matsutani M, Honda M, Okamura Y
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Sydney, Australia
• [Presentation] マイクロアレイ解析を用いた肺癌副腎転移の臓器特異性規定遺伝子の検索 (2012)
  • Author(s): 吉増達也, 尾浦正二, 太田文典, 平井慶充, 内藤古真, 中村理恵, 田中由美, 池田雅子, 川後光正, 大橋拓矢, 粉川庸三, 岡村吉隆
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] マイクロアレイを用いた肺腺癌の転移先臓器特異性決定遺伝子群の検索 (2011)
  • Author(s): 吉増達也, 尾浦正二, 太田文典, 平井慶充, 内藤古真, 中村理恵, 池田雅子, 田中由美, 川後光正, 岡村吉隆
  • Organizer: 第64回日本胸部外科学会定期学術集会
  • Place of Presentation: 名古屋
• [Presentation] Microarray analysis to detect genes predicting organotropism of lung cancer adrenal metastasis (2011)
  • Author(s): Yoshimasu T, Oura S, Ota F, Hirai Y, Naito K, Nakamura R, Nishiguchi H, Hashimoto S, Kawago M, Okamura Y
  • Organizer: 14th World Conference on Lung Cancer
  • Place of Presentation: Amsterdam
• [Presentation] 肺癌転移における臓器特異性決定機構の解析 (2010)
  • Author(s): 吉増達也, 尾浦正二, 中村理恵, 平井慶充, 内藤古真, 清井めぐみ, 宮坂美和子, 岡村吉隆
  • Organizer: 第22回Young Oncologist Conference
  • Place of Presentation: 歌山
  • Year and Date: 2010-01-29
• [Presentation] 肺癌転移における臓器特異性決定機構の解析 (2010)
  • Author(s): 吉増達也, 他
  • Organizer: 第22回 Young Oncologist Conference
  • Place of Presentation: 和歌山
  • Year and Date: 2010-01-29
• [Presentation] 組織培養法を用いた肺癌転移先臓器特異性決定遺伝子群の検索 (2010)
  • Author(s): 吉増達也, 尾浦正二, 太田文典, 中村理恵, 平井慶充, 内藤古真, 遠藤春香, 橋本沙代子, 川後光正, 岡村吉隆
  • Organizer: 第63回日本胸部外科学会定期学術集会
  • Place of Presentation: 大阪
• [Presentation] 非小細胞肺癌 in vitro 多臓器転移モデルにおける臓器親和性の検討 (2009)
  • Author(s): 吉増達也, 他
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡