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Development of bone regeneration methods for large bone defect using bone marrow derived mesenchymal stem cells.

Research Project

Project/Area Number 21390418
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SHINOMIYA Kenichi  東京医科歯科大学, 医学部, 非常勤講師 (20111594)

Co-Investigator(Kenkyū-buntansha) SOTOME Shinichi  東京医科歯科大学, 医歯学総合研究科, 寄付講座教員 (20401391)
ENOMOTO Mitsuhiro  東京医科歯科大学, 医歯学総合研究科, 寄付講座教員 (90451971)
Co-Investigator(Renkei-kenkyūsha) AE Keisuke  東京医科歯科大学, 医学部, 非常勤講師 (20376726)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords骨再生 / 骨髄間葉系幹細胞 / 分化能 / 骨形成能 / 大型骨欠損 / 分化抑制因子 / ephrin-EPHA / 骨癒合能 / 骨髄間葉系細胞 / 継代培養 / 細胞増殖 / マイクロアレイ
Research Abstract

Bone marrow mesenchymal stem cells(MSCs) have pluripotent differentiation capability and are expected to be used for regenerative medicine. However, the differentiation capability decreases with their prolonged proliferation. Moreover, our previous studies showed that the such cells had not only less bone formation capability but also suppressed bone formation. In this study, we focused on EPHA5 as the suppressor candidate from microarray and co-culture study.
We achieved 5 cm long bone regeneration using MSCs in a monkey femur bone defect model. The regenerated bone proved its bone union capability that is one of the impossible characteristics of natural bone.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (30 results)

All 2012 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 12 results) Presentation (13 results)

  • [Journal Article] Hybrid Grafting using Bone Marrow Aspirate combined with Porous beta-Tricalcium Phosphate and Trephine Bone for Lumbar Posterolateral Spinal Fusion : A Prospective, Comparative Study-Versus Local Bone Grafting-2012

    • Author(s)
      Yamada T, Yoshii T, Sotome S, Yuasa M, Kato T, Arai Y, Kawabata S, Tomizawa S, Sakaki K, Hirai T, Shinomiya K, Okawa A
    • Journal Title

      Spine

      Volume: (In press) Issue: 3 Pages: 174-179

    • DOI

      10.1097/brs.0b013e3182269d64

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the systemic administration of alendronate on bone formation in a porous hydroxyapatite/collagen composite and resorption by osteoclasts in a bone defect model in rabbits2011

    • Author(s)
      Sugata Y, Sotome S, Yuasa M, Hirano M, Shinomiya K, Okawa A
    • Journal Title

      The Journal of bone and joint surgery. British volume

      Volume: 93(4) Pages: 510-516

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 最新用語解説基礎(第35回)ハイドロキシアパタイト2010

    • Author(s)
      早乙女進一、生駒俊之
    • Journal Title

      骨粗鬆症治療

      Volume: 10(2) Pages: 53-55

    • NAID

      40018914515

    • Related Report
      2011 Final Research Report
  • [Journal Article] Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen(HAp/Col) and fibroblast growth factor-2(FGF-2)2010

    • Author(s)
      Maehara H, Sotome S, Yoshii T, Torigoe I, Kawasaki Y, Sugata Y, Yuasa M, Hirano M, Mochizuki N, Kikuchi M, Shinomiya K, Okawa A
    • Journal Title

      Journal of Orthopaedic Research

      Volume: 28(5) Pages: 677-686

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] すべての医師のための骨粗鬆症診療ガイド20102010

    • Author(s)
      早乙女進一, 四宮謙一, 大川淳
    • Journal Title

      「骨のバイオマテリアル人工骨」(綜合臨床)

      Volume: 59(4) Pages: 544-547

    • Related Report
      2011 Final Research Report
  • [Journal Article] 臨床研究の活性化と再生医療産業化の促進骨・関節領域(整形外科、歯科・口腔外科)の実施者からの提言2010

    • Author(s)
      大串始、服部耕治、牛田多加志、星和人、関矢一郎、早乙女進一、脇谷滋之、名井陽、安達伸生、加藤幸夫、河口浩之、黒田良祐、木下靱彦、梅澤明弘、田中康仁、山田陽一、袴塚康治、山我美佳、菅原桂、中島武彦
    • Journal Title

      再生医療

      Volume: 9(3) Pages: 393-400

    • Related Report
      2011 Final Research Report
  • [Journal Article] Isolation of osteogenic progenitor cells from trabecular bone for bone tissue engineering2010

    • Author(s)
      Yoshii T, Sotome S, Torigoe I, Maehara H, Sugata Y, Yamada T, Shinomiya K, Okawa A
    • Journal Title

      Tissue Engineering Part A

      Volume: 16(3) Pages: 933-942

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of gamma-ray irradiation on mechanical properties, osteoconductivity, and absorption of porous hydroxyapatite/collagen2010

    • Author(s)
      Kawasaki Y, Sotome S, Yoshii T, Torigoe I, Maehara H, Sugata Y, Hirano M, Mochizuki N, Shinomiya K, Okawa A
    • Journal Title

      Journal of Biomedical and Materials Research B Applied Biomaterials

      Volume: 92(1) Pages: 161-167

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen (HAp/Col) and fibroblast growth factor-2 (FGF-2)2010

    • Author(s)
      Maehara H-Sotome S, Shinomiya K, Okawa A, et al
    • Journal Title

      Journal of Orthopaedic Research

      Volume: 28(5) Pages: 677-686

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 【すべての医師のための骨粗霧症診療ガイド2010】「骨のバイオマテリアル 人工骨」2010

    • Author(s)
      早乙女進一, 四宮謙一, 大川淳
    • Journal Title

      綜合臨床

      Volume: 59(4) Pages: 544-547

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Isolation of osteogenic progenitor cells from trabecular bone for bone tissue engineering.2010

    • Author(s)
      Yoshii T, Sotome S, Shinomiya K, Okawa A., et al
    • Journal Title

      Tissue Engineering Part A 16(3)

      Pages: 933-942

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of gamma-ray irradiation on mechanical properties, osteoconductivity, and absorption of porous hydroxyapatite/collagen.2010

    • Author(s)
      Kawasaki Y, Sotome S, Shinomiya K, Okawa A., et al
    • Journal Title

      Journal of Biomedical and Materials Research B Applied Biomaterials 92(1)

      Pages: 161-167

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates2009

    • Author(s)
      Torigoe I, Sotome S, Tsuchiya A, Yoshii T, Maehara H, Sugata Y, Ichinose S, Shinomiya K, Okawa A
    • Journal Title

      Tissue Engineering Part A

      Volume: 15(7) Pages: 1489-1499

    • Related Report
      2011 Final Research Report
  • [Journal Article] Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates.2009

    • Author(s)
      Torigoe I, Sotome S, Shinomiya K, Okawa A., et al
    • Journal Title

      Tissue Engineering Part A 15(7)

      Pages: 1489-1499

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid Grafting using Bone Marrow Aspirate combined with Porous beta-Tricalcium Phosphate and Trephine Bone for Lumbar Posterolateral Spinal Fusion : A Prospective, Comparative Study-Versus Local Bone Grafting

    • Author(s)
      Yamada T, Yoshii T, Sotome S, Yuasa M, Kato T, Arai Y, Kawabata S, Tomizawa S, Sakaki K, Hirai T, Shinomiya K, Okawa A
    • Journal Title

      Spine

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the systemic administration of alendronate on bone formation in a porous hydroxyapatite/collagen composite and resorption by osteoclasts in a rabbit bone defect model

    • Author(s)
      Sugata Y, Sotome S, Shinomiya K, Okawa A, et al.
    • Journal Title

      Journal of Bone and Joint Surgery British Edition

      Volume: In press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen(HAp/Col)and fibroblast growth factor-2(FGF-2).

    • Author(s)
      Maehara H, Sotome S, Shinomiya K, Okawa A., et al
    • Journal Title

      Journal of Orthopaedic Research (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨移植治療の歴史と進歩多孔質ハイドロキシアパタイト・コラーゲン複合体(HAp/Col)の開発と臨床応用2011

    • Author(s)
      早乙女進一、阿江啓介、若林良明、四宮謙一、平野昌弘、石突正文、森岡秀夫;松本誠一、中村孝志、阿部哲士、別府保男
    • Organizer
      第84回日本整形外科学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 骨髄間葉系細胞を用いた骨再生法の大型骨欠損再生に対する有効性-サル大腿骨大欠損モデルを用いた検討2011

    • Author(s)
      早乙女進一、湯浅将人、山田剛史、正岡智和、吉井俊貴、四宮謙一、大川淳
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      群馬
    • Related Report
      2011 Final Research Report
  • [Presentation] β-TCP/bonemarrow hybridの骨形成能に対するdexamethasoneの効果2011

    • Author(s)
      正岡智和、湯浅将人、山田剛史、大川淳、森田定雄、早乙女進一
    • Organizer
      第26回日本整形外科学会基礎学術集会
    • Place of Presentation
      群馬
    • Related Report
      2011 Final Research Report
  • [Presentation] 骨髄間葉系細胞を用いた骨再生法の大型骨欠損再生に対する有効性-サル大腿骨大欠損モデルを用いた検討-2011

    • Author(s)
      早乙女進一、湯浅将人、山田剛史、正岡智和、吉井俊貴、四宮謙一、大川淳
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      群馬
    • Related Report
      2011 Annual Research Report
  • [Presentation] β-TCP/bone marrow hybridの骨形成能に対するdexamethasoneの効果2011

    • Author(s)
      正岡智和、湯浅将人、山田剛史、大川淳、森田定雄、早乙女進一
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      群馬
    • Related Report
      2011 Annual Research Report
  • [Presentation] BMPとデキサメサゾン併用による骨形成能に関する検討2010

    • Author(s)
      湯浅将人、早乙女進一、吉井俊貴、富沢将司、加藤剛、川端茂〓、四宮謙一、大川淳
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 温熱処理骨と骨髄由来間質細胞導入β-TCPを用いた大型犬の骨欠損モデルにおける再生能2010

    • Author(s)
      小柳広高、阿江啓介、早乙女進一、前原秀二、菅田祐美、湯浅将人、山田剛史、正岡智和、船内雄生、四宮謙一、大川淳
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 多孔質ハイドロキシアパタイト・コラーゲン複合体(HAp/Col)の開発と臨床応用2009

    • Author(s)
      早乙女進一、土谷明男、前原秀二、菅田祐美、阿江啓介、若林良明、四宮謙一、上坂優子、高山知士、望月直美、塩谷慎吾、平野昌弘、石突雅文、森岡秀雄、松本誠一、中村孝志、阿部哲、別府保男
    • Organizer
      第27回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Final Research Report
  • [Presentation] ビスフォスフォネートが生体吸収性骨補填剤に吸収と骨形成に与える影響(第2報)2009

    • Author(s)
      菅田祐美、早乙女進一、前原秀二、川崎雄一、湯浅将人、望月直美、平野昌弘、四宮謙一、大川淳
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 温熱処理骨と骨髄由来間質細胞導入β-TCPを用いた大型犬の骨欠損モデルにおける骨再生2009

    • Author(s)
      小柳広高、阿江啓介、早乙女進一、前原秀二、菅田祐美、湯浅将人、正岡智和、山田剛史、船内雄生、四宮謙一、大川淳
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 多孔質ハイドロキシアパタイト/コラーゲン複合体(HAp/Col)のosteogenic protein-1(OP-1)担体としての有用性に関する検討2009

    • Author(s)
      前原秀二、早乙女進一、菅田祐美、湯浅将人、正岡智和、山田剛史、平野昌弘、望月直美、四宮謙一、大川淳
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] γ線滅菌による多孔質ハイドロキシアパタイト・コラーゲン複合体(HAp/Col)の機械的強度、吸収性、および骨形成能への影響2009

    • Author(s)
      早乙女進一、川崎雄一、吉井俊貴、鳥越一郎、前原秀二、菅田祐美、望月直美、平野昌弘、四宮謙一、大川淳
    • Organizer
      第24回日本整形外科学会基礎学術集会
    • Place of Presentation
      横浜
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 多孔質ハイドロキシアパタイト・コラーゲン複合体(HAp/Col)の開発と臨床応用2009

    • Author(s)
      早乙女進一、四宮謙一, 他
    • Organizer
      第27回日本骨代謝学会(シンポジウム)
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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