The interaction of cancer cell and stromal cell in urinary cancer-The approach of stromal therapy-

• Project/Area Number: 21390442
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: The University of Tokushima
• Principal Investigator: KANAYAMA Hiro-omi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10214446)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Masayuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 講師 (50325255) ; FUKUMORI Tomoharu 徳島大学, 病院, 講師 (10314874) ; NAKATSUJI Hiroyoshi 徳島大学, 病院, 助教 (50437638)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
• Keywords: 腫瘍学 / 癌 / 間質細胞相互作用 / HGF / Stromal Therapy / 癌・間質細胞 / Storomal Therapy / 癌・間質細胞相互作用

Research Abstract

In Micro array assay, we made a comparative study on bladder cancer cell line 5637 treated with or without hepatocyto growth factor(HGF), focus ing attention on toll like receptor 4(TLR4), Matrix metal loproteases-1(MMP-1) and MMP-10 of 26 molecule, more than 2-fold change(3down-regulation, 23up-regulation). We also assessed the TLR4, MMP-1 and MMP-10 involvement in the invasion and proliferation of bladder cancer.
TLR4, MMP-1 and MMP-10 mRNA levels i n invasive bladder cancer cells treated with HGF were dose-dependently elevated in real-time PCR. The escalation of MMP-1and MMP-10 activity was inhibited by knockdown of TLR4 in 5637 cells treated with HGF.
In conclusion, we speculate that the effect of HGF lead to enhance the ability of invasiveness by escalating the activity of MMP through the signal of TLR4-NFκB.

Research Products

• [Journal Article] Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts (2011)
  • Author(s): Shiirevnyamba A, Yano S, et al.
  • Journal Title: Br J Cancer Volume: 104 Issue: 3 Pages: 505-513
  • DOI: 10.1038/sj.bjc.6606070
  • Peer Reviewed
• [Journal Article] Enhancement of osteoclastgenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts (2011)
  • Author(s): A Shiirevnyamba
  • Journal Title: British Journal of Cancer Volume: 104 Pages: 505-513
  • Peer Reviewed
• [Journal Article] Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts (2011)
  • Author(s): Shiirevnyamba A
  • Journal Title: Br J Cancer Volume: 104 Pages: 505-513
  • Peer Reviewed
• [Journal Article] Clinical significance of Galectin-3 in clear cell renal cell carcinoma (2010)
  • Author(s): Sakaki S、Fukumori T, Fukawa T, Essam Elsamman, A Shiirevnyamba, Nakatsuji H, Kanayama H
  • Journal Title: The Journal of Medical Investigation Volume: 57・1, 2 Pages: 152-157
  • NAID: 130004465193
  • URL: http://medical.med.tokushima-u.ac.jp/jmi/vol57/pdf/v57_n1-2_p152.pdf
  • Peer Reviewed
• [Journal Article] The Role of Actinin-4 in Bladder Cancer lnvasion (2010)
  • Author(s): Koizumi T、Nakatsuji H, Fukawa T, Shiirevnyamba A, Fukumori T, Takahashi M, Kanayama H
  • Journal Title: The Role of Actinin-4 in Bladder Cancer lnvasion UROLOGY Volume: 75 Pages: 357-364
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19969329
  • Peer Reviewed
• [Journal Article] The Role of Actinin-4 in Bladder Cancer Invasion (2010)
  • Author(s): Takahiro Koizumi
  • Journal Title: UROLOGY Volume: 75 Pages: 357-364
  • Peer Reviewed
• [Journal Article] Clinical significance of Galectin-3 in clear cell renal cell carcinoma (2010)
  • Author(s): Manabu S
  • Journal Title: The Journal of Medical Investigation Volume: 57・1,2 Pages: 152-157
  • Peer Reviewed
• [Journal Article] Clinical significance of Galectin-3 in clear cell renal cell carcinoma (2010)
  • Author(s): Sakaki M
  • Journal Title: J Med Invest 57 Pages: 152-157
  • Peer Reviewed
• [Journal Article] The role of actinin-4 in bladder cancer invasion (2010)
  • Author(s): Koizumi T
  • Journal Title: Urology 75 Pages: 357-364
  • Peer Reviewed
• [Journal Article] Kanayama H Effect of Vascular Endothelial Growth Factor and Its Receptor Inhibitor on Proliferation and Invasion in Bladder Cancer (2009)
  • Author(s): Nakanishi R, Oka N, Nakatsuji H, Koizumi T, Sakaki M, Takahashi M, Fukumori T
  • Journal Title: Urologia Internationalis Volume: 83 Issue: 1 Pages: 98-106
  • DOI: 10.1159/000224877
  • Peer Reviewed
• [Journal Article] Effect of Vascular Endothelial Growth Factor and Its Receptor Inhibitor on Proliferation and Invasion in Bladder Cancer (2009)
  • Author(s): Nakanishi R
  • Journal Title: Urologia Internationalis 83 Pages: 98-106
  • Peer Reviewed
• [Journal Article] Transperitoneal versus retroperitoneal laparoscopic radical nephrectomy : A comparative study (2009)
  • Author(s): Taue R
  • Journal Title: International Journal of Urology 16 Pages: 263-267
  • Peer Reviewed
• [Presentation] The role of Actinin-4 in Bladder Cancer Metastasis (2011)
  • Author(s): Nakatsuji H
  • Organizer: SIU
  • Place of Presentation: ICC BERLIN(ドイツベルリン)
  • Year and Date: 2011-10-17
• [Presentation] The role of Actinin-4 in Bladder Cancer Metastasis (2011)
  • Author(s): Nakatsuji H
  • Organizer: SIU
  • Place of Presentation: ICC(BERLIN)
  • Year and Date: 2011-10-17
• [Presentation] The involvement of TLR4 on the increase of invasion and proliferation ability in bladder cancer treated (2011)
  • Author(s): 中逵弘能
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋コングレスセンター(名古屋市)
  • Year and Date: 2011-10-04
• [Presentation] The involvement of TLR4 on the increase of invasion and proliferation ability in bladder cancer treated with HGF (2011)
  • Author(s): 中逵弘能
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋コングレスセンクー(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] Indentification and characterization of RCCDH as a novel molecular target for clear renal cell carcinoma (2011)
  • Author(s): 布川朋也
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋コングレスセンター(名古屋市)
  • Year and Date: 2011-10-03
• [Presentation] Indentification and characterization of RCCDH as a novel molecular target for clear renal cell carcinoma (2011)
  • Author(s): 布川朋也
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋コングレスセンター(愛知県)
  • Year and Date: 2011-10-03
• [Presentation] Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts (2011)
  • Author(s): SAvilmed
  • Organizer: AUA
  • Place of Presentation: Washington Convention center(アメリカワシントン)
  • Year and Date: 2011-05-17
• [Presentation] Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts (2011)
  • Author(s): S Avilmed
  • Organizer: AUA
  • Place of Presentation: Washington Convention center (USA)
  • Year and Date: 2011-05-17
• [Presentation] Participation of N-caderin in bladder cancer (2011)
  • Author(s): アビルメド・シーレンヤンバ
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-04-24
• [Presentation] Participation-of N-caderin in bladder cancer (2011)
  • Author(s): アビルメド・シーレンヤンバ
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-04-24
• [Presentation] HGFによる膀胱がん浸潤亢進におけるToll like receptor 4(TLR4)の関与 (2011)
  • Author(s): 中逵弘能
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-04-23
• [Presentation] HGFによる膀胱がん浸潤亢進におけるToll like receptor 4 (TLR4)の関与 (2011)
  • Author(s): 中逵弘能
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-04-23
• [Presentation] ゲノム情報解析による腎細胞癌標的治療分子の同定およびその機能解析 (2011)
  • Author(s): 布川朋也
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-04-22
• [Presentation] ゲノム情報解析による腎細胞癌標的治療分子の同定およびその機能解析 (2011)
  • Author(s): 布川朋也
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-04-22
• [Presentation] MicroRNAs are reproducibly deregulated in prostate carcinoma and are potential regulators of signal transduction pathways like PI3-kinase or MAP-kinase pathways (2011)
  • Author(s): S Avirmed
  • Organizer: EAU
  • Place of Presentation: Austria Centre Vienna(ACV)
  • Year and Date: 2011-03-20
• [Presentation] MicroRNAs are reproducibly deregulated in prostate carcinoma and are potential regulators of signal transduction pathways like PI3-kinase or MAP-kinase pathways (2011)
  • Author(s): S Avirmed
  • Organizer: EAU
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-03-20
• [Presentation] Participation of N-cadherin in bladder Cancer (2011)
  • Author(s): アビルメド・シーレンヤンバ
  • Organizer: 泌尿器科分子・細胞研究会
  • Place of Presentation: ホテルグリーンパーク津(津市)
  • Year and Date: 2011-03-12
• [Presentation] HGFによる膀胱がん浸潤亢進におけるToll like receptor 4(TLR4)の関与 (2011)
  • Author(s): 中逵弘能
  • Organizer: 泌尿器科分子・細胞研究会
  • Place of Presentation: ホテルグリーンパーク津(津市)
  • Year and Date: 2011-03-12
• [Presentation] Identification and characterization of RCCMP as a novel molecular target for clear cell-renal cell (2010)
  • Author(s): 布川朋也
  • Organizer: 日本泌尿器科学会西日本総会
  • Place of Presentation: 鹿児島県民交流センター(鹿児島市)
  • Year and Date: 2010-11-06
• [Presentation] Identification and characterization of RCCMP as a novel molecular target for clear cell-renal cell carcinoma (2010)
  • Author(s): 布川朋也
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪(大阪市)
  • Year and Date: 2010-09-24
• [Presentation] The involvement of Plakophilin in bladder cancer invasion (2010)
  • Author(s): 中逵弘能
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪(大阪市)
  • Year and Date: 2010-09-22
• [Presentation] 予後因子 (2010)
  • Author(s): 金山博臣
  • Organizer: 腎癌研究会記念大会
  • Place of Presentation: フォーシーズンズホテル椿山荘(東京都)
  • Year and Date: 2010-07-18
• [Presentation] 膀胱癌浸潤におけるプラコフィリン2の関与 (2010)
  • Author(s): 中逵弘能
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: いわて県民情報交流センター(盛岡市)
  • Year and Date: 2010-04-30
• [Presentation] The Involvement of Actinin-4 in Bladder cancer Metastasi (2009)
  • Author(s): 中逵弘能
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-03
• [Presentation] Change of gene expressions of prostate cancer cells by direct contact with osteoblasts (2009)
  • Author(s): アビルメド・シーレンヤンバ
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-10-03
• [Presentation] Amelioration of Acute Tubular Necrosis in Ischemic Acute Renal Failure was Impaired in Mice Lucking Hypoxia Inducible Factor-1αGene (2009)
  • Author(s): Yamagchi K
  • Organizer: WCN
  • Place of Presentation: Mirano
  • Year and Date: 2009-05-23
• [Presentation] Evaluation of different subtypes of renal tumors on double-phase helical CT (2009)
  • Author(s): Takahashi M
  • Organizer: AUA
  • Place of Presentation: Hilton Chicago, Hyatt Regency, McCormick Place(Chicago)
  • Year and Date: 2009-04-27
• [Presentation] 膀胱癌の浸潤・増殖に対するActinin-4の生物学的機能の検討 (2009)
  • Author(s): 小泉貴裕
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2009-04-18
• [Presentation] 腎虚血-再灌流障害に対するHif-1α(hypoxia inducible factor-1α)が与える影響に関する検討 (2009)
  • Author(s): 山口邦久
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2009-04-16