The analysis of Ni allergy development and the therapeutic strategy in prosthodontics

• Project/Area Number: 21390518
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: ICHIKAWA Tetsuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90193432)
• Co-Investigator(Kenkyū-buntansha): ISHIMARU Naozumi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (60314879) ; WATANABE Megumi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (40380050) ; KASHIWABARA Toshiya 徳島大学, 病院, 助教 (90274232)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 金属アレルギー / 樹状細胞 / MAPキナーゼ / ニッケル / T細胞

Research Abstract

We analyzed Ni allergy symptoms in the mouse model which we produced. The major populations of infiltrated immune cells around the allergic region in skin were CD3 positive cells(T cells) and CD11c/ MHC classII positive cells(dendritic cells). p38/ MKK6 pathway in dendritic cells was activated by Ni stimulation both in vivo and in vitro.

Research Products

• [Journal Article] A novel DC therapy with manipulation of MKK6 gene on nickel allergy in mice (2011)
  • Author(s): Megumi Watanabe, Naozumi Ishimaru, Meinar Nur Ashrin, Rieko Arakaki, Akiko Yamada, Tetsuo Ichikawa, and Yoshio Hayashi
  • Journal Title: PLoS ONE Volume: 6(4) Issue: 4 Pages: e19017-e19017
  • DOI: 10.1371/journal.pone.0019017
  • Peer Reviewed
• [Journal Article] A novel therapy with manipulation of MKK6 gene on nickel allergy in mice
  • Author(s): Megumi Watanabe, et al.
  • Journal Title: PLoS ONE Volume: (In press)
  • Peer Reviewed
• [Presentation] The Role of MKK6/ p38 Signal Cascade in Dendritic Cells in Mickel Allergy Mouse Model (2011)
  • Author(s): Megumi Watanabe
  • Organizer: Kalimantan Symposia
  • Place of Presentation: バリクパパン(インドネシア)
  • Year and Date: 2011-12-11
• [Presentation] The Role of MKK6/p38 Signal Cascade in Dendritic Cells in Nickel Allergy Mouse Model (2011)
  • Author(s): Watanabe, M
  • Organizer: Kalimantan Symposia
  • Place of Presentation: バリクパパン(インドネシア)
  • Year and Date: 2011-12-11
• [Presentation] インプラント材料とアレルギー (2011)
  • Author(s): 渡邉恵
  • Organizer: 第41回公益社団法人日本口腔インプラント学会・学術大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-09-15
• [Presentation] インプラント材料とアレルギー (2011)
  • Author(s): 渡邉恵
  • Organizer: 第41回公益社団法人日本口腔インプラント学会・学術大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)(招待講演)
  • Year and Date: 2011-09-15
• [Presentation] I Extremely LowFrequency Pulsed Magnetic Fields Accelerate Osteoblast Differentiation (2011)
  • Author(s): Megumi Watanabe, Tetsuo Ichikawa
  • Organizer: ICP 14th Biennial Meeting
  • Place of Presentation: ハワイ(米国)
  • Year and Date: 2011-09-10
• [Presentation] Extremely Low Frequency Pulsed Magnetic Fields Accelerate Osteoblast Differentiation (2011)
  • Author(s): Watanabe, M, Ichikawa, T
  • Organizer: ICP 14th Biennial Meeting
  • Place of Presentation: ハワイ(米国)
  • Year and Date: 2011-09-10
• [Presentation] Analysis of Molecular Mechanism for Pathogenesis of Nickel Allergy (2011)
  • Author(s): Meinar Nur Asrin, Megumi Watanabe, Tetsuo Ichikawa et al.
  • Organizer: 日本補綴歯科学会第120回記念学術大会
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2011-05-20
• [Presentation] Analysis of Molecular Mechanism for Pathogenesis of Nickel Allergy (2011)
  • Author(s): Meinar Nur Ashrin, Watanabe Megumi, Ichikawa Tetsuo
  • Organizer: 社団法人日本補綴歯科学会第120回記念学術大会
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 2011-05-20
• [Presentation] Analysis of Molecular Mechanism for Pathogenesis of Metal Allergy (2010)
  • Author(s): Meinar Nur Asrin, Megumi Watanabe, Tetsuo Ichikawa et al.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: クタ(インドネシア)
  • Year and Date: 2010-12-17
• [Presentation] Analysis of Molecular Mechanism for Pathogenesis of Metal Allergy (2010)
  • Author(s): Meinar Nur Ashrin, et al.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Kuta, Bali
  • Year and Date: 2010-12-17
• [Presentation] Extremely Low Frequency Pulsed Magnetic Fields Accelerate Osteoblast Differentiation (2010)
  • Author(s): Megumi Watanabe, Tetsuo Ichikawa
  • Organizer: European Academy of Osseointegration
  • Place of Presentation: グラスゴー(英国)
  • Year and Date: 2010-10-07
• [Presentation] ニッケルアレルギーモデルマウス樹状細胞におけるシグナル伝達の解析 (2010)
  • Author(s): 渡邉恵
  • Organizer: 第2回「口腔環境制御研究」カテゴリー集会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2010-02-10
• [Presentation] ニッケルアレルギーモデルマウス樹状細胞におけるシグナル伝達の解析 (2009)
  • Author(s): 渡邉恵
  • Organizer: 口腔からQOL向上を目指す連携研究ミニシンポジウム
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2009-10-16
• [Presentation] ニッケルアレルギーモデルマウス樹状細胞におけるシグナル伝達の解析 (2009)
  • Author(s): 渡邉恵
  • Organizer: 口腔からQOLI向上を目指す連携研究ミニシンポジウム
  • Place of Presentation: 徳島大学(徳島市)
  • Year and Date: 2009-10-16
• [Presentation] MKK6/ p38 Signal Transduction Cascades Regulate Nickel Allergy in the Model Mouse (2009)
  • Author(s): Watanabe, M, Ichikawa, T.
  • Organizer: International College of Prosthodontics
  • Place of Presentation: ケープタウン(南アフリカ)
  • Year and Date: 2009-09-10
• [Presentation] MKK6/p38 Signal Transduction Cascades Regulate Nickel Allergy in the Model Mouse (2009)
  • Author(s): Watanabe, M.
  • Organizer: International College of Prosthodontics
  • Place of Presentation: Cape Town
  • Year and Date: 2009-09-10
• [Presentation] ニッケルアレルギーモデルマウス樹状細胞におけるシグナル伝達の解析 (2009)
  • Author(s): 渡邉恵, 市川哲雄
  • Organizer: 日本補綴歯科学会第118回学術大会
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2009-06-06
• [Presentation] ニッケルアレルギーモデルマウス樹状細胞におけるシグナル伝達の解析 (2009)
  • Author(s): 渡邉恵
  • Organizer: (社)日本補綴歯科学会第118回学術大会
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2009-06-06