Evaluation on clarification of transport system of transition metallic ions inside body-defense cells and its association with bio-safety

• Project/Area Number: 21390526
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: TAIRA Masayuki 岩手医科大学, 歯学部, 准教授 (60179398)
• Co-Investigator(Kenkyū-buntansha): KAGIYA Tadayoshi 岩手医科大学, 歯学部, 助教 (30405774)
• Co-Investigator(Renkei-kenkyūsha): NEZU Akashi 岩手医科大学, 歯学部, 講師 (40264056) ; SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 歯学 / 生体材料 / 細胞・組織 / 遷移金属イオン / 遺伝子 / 銅イオンシャペロン / 銅イオントランスポーター / 損傷遺伝子修復酵素

Research Abstract

We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.

Research Products

• [Journal Article] Macrophage reaction against sub-micron titanium particles (2012)
  • Author(s): Taira, M., Sasaki, M. and Kimura, S.
  • Journal Title: Interface Oral Health Science 2011 Volume: Part6 Pages: 283-284
  • DOI: 10.1007/978-4-431-54070-0_84
  • ISBN: 9784431540694, 9784431540700
  • Peer Reviewed
• [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage. recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions (2011)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M. and Kimura, S.
  • Journal Title: Nano-Biomedicine Volume: 3 Pages: 294-299
  • NAID: 130004555811
  • URL: https://www.jstage.jst.go.jp/article/nano/3/2/3_2_294/_article
  • Peer Reviewed
• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper(2+) ions (2011)
  • Author(s): Taira M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S.
  • Journal Title: Dental Mater. J. Volume: 30 Pages: 293-299
  • NAID: 10029136627
  • URL: http://dx.doi.org/10.4012/dmj.2010-088
  • Peer Reviewed
• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper (2+) ions (2011)
  • Author(s): Taira, M., 他6名
  • Journal Title: Dental Materials Journal Volume: 30 Issue: 3 Pages: 293-299
  • DOI: 10.4012/dmj.2010-088
  • NAID: 10029136627
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions (2011)
  • Author(s): Taira, M., 他3名
  • Journal Title: Nano-Biomed Volume: 3 Pages: 294-299
  • NAID: 130004555811
  • Peer Reviewed
• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 Cu(2+) ions (2011)
  • Author(s): Taira, M., Shimoyama Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H., Kimura, S.
  • Journal Title: Dental Materials Journal Volume: 30(受理済み)
  • Peer Reviewed
• [Journal Article] 歯科技工における粉塵について知っておきたいこと (2010)
  • Author(s): 平雅之、齋藤設雄
  • Journal Title: 日本歯技 Volume: 494 Pages: 33-40
  • URL: http://www.nichigi.or.jp/about_nichigi/nihonshigi.html
• [Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium (2010)
  • Author(s): Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S. and Ishizeki, K.
  • Journal Title: J. Oral Tissue Eng. Volume: 8 Pages: 102-106
  • NAID: 130004941170
  • URL: https://www.jstage.jst.go.jp/article/jarde/8/2/8_2_102/_article
  • Peer Reviewed
• [Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles (2010)
  • Author(s): Taira, M., Kagiya, T., Harada, H., Sasaki, M., 他4名
  • Journal Title: J. Mater. Sci. Mater. Med. Volume: 21 Issue: 1 Pages: 267-275
  • DOI: 10.1007/s10856-009-3834-x
  • Peer Reviewed
• [Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium (2010)
  • Author(s): Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S., Ishizeki, K
  • Journal Title: Journal of Oral Tissue Engineering Volume: 8 Pages: 102-106
  • NAID: 130004941170
  • Peer Reviewed
• [Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles (2010)
  • Author(s): Taira, M., Kagiya, T., Harada, H., Sasaki, M., 他4名
  • Journal Title: J.Mater.Sci.Mater.Med. 21 Pages: 267-275
  • Peer Reviewed
• [Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW264 (2009)
  • Author(s): Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S. and Araki, Y.
  • Journal Title: Nano-Biomedicine Volume: 1 Pages: 59-69
  • NAID: 130004555790
  • URL: https://www.jstage.jst.go.jp/article/nano/1/1/1_1_59/_article
  • Peer Reviewed
• [Journal Article] Characterization of aerosols and fine particles produced in dentistry and their health risk assessments (2009)
  • Author(s): Taira, M., Sasaki, M., Kimura, S. and Araki, Y.
  • Journal Title: Nano-Biomedicine Volume: 1 Pages: 9-15
  • NAID: 130004555793
  • URL: https://www.jstage.jst.go.jp/article/nano/1/1/1_1_9/_article
  • Peer Reviewed
• [Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions (2009)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., 他4名
  • Journal Title: J. Oral Tissue Eng Volume: 7 Pages: 107-120
  • NAID: 130004456605
  • URL: https://www.jstage.jst.go.jp/article/jarde/7/2/7_2_107/_article
  • Peer Reviewed
• [Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions (2009)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., 他4名
  • Journal Title: J.Oral Tissue Eng. 7 Pages: 107-120
  • NAID: 130004456605
  • Peer Reviewed
• [Journal Article] Characterization of aerosols and fine particles produced in dentistry and their health risk assessments (2009)
  • Author(s): Taira, M., Sasaki, M., Kimura, S., Araki, Y.
  • Journal Title: Nano-Bioscience 1 Pages: 9-15
  • NAID: 130004555793
  • Peer Reviewed
• [Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW264 (2009)
  • Author(s): Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S., Araki, Y.
  • Journal Title: Nano-Bioscience 1 Pages: 59-69
  • NAID: 130004555790
  • Peer Reviewed
• [Presentation] THP-1細胞を用いたチタン微粒子、銅イオンとTEGDMAモノマーの細胞障害性の評価 (2011)
  • Author(s): 平雅之
  • Organizer: 日本動物実験代替法学会第24回大会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-11-12
• [Presentation] THP-1細胞を用いたチタン微粒子、銅イオンとTEGDMAモノマーの細胞障害性の評価 (2011)
  • Author(s): 平雅之
  • Organizer: 日本動物実験代替法学会第24回大会
  • Place of Presentation: 仙台市(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] EGDMAに曝された時のTHP-1単球の異物代謝並びに炎症性サイトカイン関連遺伝子の発現評価 (2011)
  • Author(s): 平雅之
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山市
  • Year and Date: 2011-10-22
• [Presentation] TEGDMAに曝された時のTHP-1単球の異物代謝並びに炎症性サイトカイン関連遺伝子の発現評価 (2011)
  • Author(s): 平雅之
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 郡山市
  • Year and Date: 2011-10-22
• [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について (2011)
  • Author(s): 鍵谷忠慶、平雅之, 他2名
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-02
• [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について (2011)
  • Author(s): 鍵谷忠慶, 他3名
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-02
• [Presentation] Histological and TEM observation of subcutaneous tissues exposed to particulate pure metals (2011)
  • Author(s): Saitoh, S., Taira, M., 他2名
  • Organizer: International Dental Materials Congress 2011(IDMC2011)
  • Place of Presentation: Soul, Korea
  • Year and Date: 2011-05-28
• [Presentation] Histological and TEM observation of subcutaneous tissues exposed to particulate pure metals (2011)
  • Author(s): Saitoh, S., 他3名
  • Organizer: International Dental Materials Congress 2011 (IDMC2011)
  • Place of Presentation: Soul, Korea
  • Year and Date: 2011-05-28
• [Presentation] 3種類の純金属に対するマウス背部皮下における生体組織反応の評価 (2010)
  • Author(s): 齋藤設雄, 佐々木かおり, 根津尚史, 平雅之, 下山佑, 佐々木実, 木村重信, 石関清人
  • Organizer: 岩手医科大学歯学会第36回総会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-12-04
• [Presentation] 低毒性IC90濃度の銅イオンがマクロファージの蛋白発現に及ぼす影響評価 (2010)
  • Author(s): 平雅之
  • Organizer: 第32回バイオマテリアル学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-11-30
• [Presentation] 皮下埋入した金属材料に対する生体組織反応の基礎的評価 (2010)
  • Author(s): 齋藤設雄, 佐々木かおり, 根津尚史, 平雅之
  • Organizer: 第56回日本歯科理工学会学術講演会
  • Place of Presentation: 岐阜市
  • Year and Date: 2010-10-10
• [Presentation] マクロファージ内における銅イオン輸送蛋白質の遺伝子発現解析 (2010)
  • Author(s): 平雅之, 佐々木かおり, 齋藤設雄, 根津尚史
  • Organizer: 第56回日本歯科理工学会学術講演会
  • Place of Presentation: 岐阜市
  • Year and Date: 2010-10-09
• [Presentation] 皮下埋入した金属材料が生体組織に及ぼす影響 (2010)
  • Author(s): 齋藤設雄, 佐々木かおり, 根津尚史, 平雅之
  • Organizer: ナノ・バイオメディカル学会第3回大会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-09-17
• [Presentation] 高濃度の銅イオンがマクロファージに及ぼす傷害作用に関する研究 (2009)
  • Author(s): 平雅之、荒木吉馬
  • Organizer: 第31回バイオマテリアルズ学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2009-11-17
• [Presentation] IC10%濃度の銅イオンで培養したマクロファージのTEM形態観察と細胞内銅イオン量のEDX測定 (2009)
  • Author(s): 平雅之、佐々木かおり、齋藤設雄、根津尚史、荒木吉馬
  • Organizer: 第54回日本歯科理工学会学術講演会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2009-10-01
• [Presentation] IC50ニッケルイオンがヒト間葉系幹細胞の遺伝子発現に及ぼす影響評価 (2009)
  • Author(s): 平雅之, 佐々木かおり, 他3名
  • Organizer: 第7回日本再生歯科医学会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-09-12
• [Presentation] IC50濃度ニッケルイオンがLPS活性化マクロファージのDNA傷害修復関連遺伝子の発現に及ぼす影響 (2009)
  • Author(s): 平雅之、佐々木実、木村重信
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-10
• [Presentation] IC50%ニッケルイオンとLPSがマクロファージの全遺伝子発現に及ぼす影響評価 (2009)
  • Author(s): 平雅之、荒木吉馬
  • Organizer: ナノ・バイオメディカル学会第1回大会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-07-18
• [Presentation] 銅イオンがマウス多形核白血球(好中球)の活性酸素産生に及ぼす濃度依存的影響 (2009)
  • Author(s): 平雅之、佐々木かおり、齋藤設雄、根津尚史、荒木吉馬
  • Organizer: 第53回日本歯科理工学会学術講演会
  • Place of Presentation: 東京都
  • Year and Date: 2009-04-11
• [Book] 9章ナノ粒子の細胞毒性評価 (2011)
  • Author(s): 平雅之, 他57名
  • Publisher: フロンティア出版
• [Book] ナノ粒子のリスク評価と安全性対策 初版, 亘理文夫 監修 9 ナノ粒子の細胞毒性評価 (2010)
  • Author(s): 平雅之
  • Total Pages: 6
  • Publisher: フロンティア出版
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