Identification and characterization of factors involved in the resistance to Marek' s disease in chickens and wild waterfowls

• Project/Area Number: 21405034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Kazuhiko 北海道大学, 大学院・獣医学研究科, 教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): KONNAI Satoru 北海道大学, 大学院・獣医学研究科, 准教授 (40396304)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: マレック病 / マレック病ウイルス / インターフェロンγ / 野生水禽 / マガン / インターフエロンγ

Research Abstract

To study the molecular mechanism(s) for the natural resistance to Marek' s disease (MD) in wild waterfowls and genetically resistant lines of chickens, the regulatory mechanisms of the expression of interferon y (IFNy), which plays an important role in the cell-mediated immunity against MD virus (MDV), were analyzed by using the conventional luciferase reporter assay system. The promoter activity was more strongly repressed in white-fronted geese than chickens when Meq, an MDV oncoprotein, was co-expressed. This result suggests that, in white-fronted geese, reduced expression of IFNy by Meq at early after MDV infection could result in the lower degree of T cell activation (activated T cells are targets for MDV) to reduce the chances for the birds to develop MD.

Research Products

• [Journal Article] Analysis of transcriptional activities of the Meg proteins present in highly virulent Marek's disease virus strains (2011)
  • Author(s): Murata, S., Konnai, S., & Ohashi, K.
  • Journal Title: RB1B and Md5. Virus genes Volume: 43 Pages: 66-71
  • Peer Reviewed
• [Journal Article] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布 (2011)
  • Author(s): 大橋和彦, 松原綾子, 村田史郎, 今内覚
  • Journal Title: J. Vet. Med Volume: 64 Pages: 21-26
• [Journal Article] Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-Ll blockade (2011)
  • Author(s): Ikebuchi, R., Konnai, S., Shirai, T., Sunden, Y., Murata, S., Onuma, M., & Ohashi, K.
  • Journal Title: Vet. Res Volume: 42 Pages: 103-103
  • Peer Reviewed
• [Journal Article] Analysis of transcriptional activities of the Meq proteins present in highly virulent Marek's disease virus strains, RB1B and Md5 (2011)
  • Author(s): Murata S., et al.
  • Journal Title: Virus Genes Volume: 43 Issue: 1 Pages: 66-71
  • DOI: 10.1007/s11262-011-0612-x
  • Peer Reviewed
• [Journal Article] Surveillance or Marek's disease virus in migratory and sedentary birds in Hokkaido, Japan (2011)
  • Author(s): Murata S., et al.
  • Journal Title: Veterinary Journal Volume: 192(印刷中) Issue: 3 Pages: 538-540
  • DOI: 10.1016/j.tvjl.2011.07.006
  • Peer Reviewed
• [Journal Article] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布 (2011)
  • Author(s): 大橋和彦, ら
  • Journal Title: Journal of Veterinary Medicine Volume: 64 Pages: 21-26
• [Journal Article] Two novel Salp15-like immunosuppressant genes from salivary glands of Ixodes persulcatus Schulze tick (2010)
  • Author(s): Shirai, T., Konnai, S., Ikebuchi, R., Okagawa, T., Suzuki, S., Sunden, Y., Onuma, M., Murata, S., & Ohashi, K.
  • Journal Title: Insect Mal. Biol Volume: 19 Pages: 359-365
  • Peer Reviewed
• [Journal Article] Cytokine profiles in chickens infected with virulent and avirulent Marek' s disease viruses : interferon-gamma is a key factor in the protection of Marek' s disease by vaccination (2009)
  • Author(s): Kano, R., Konnai, S., Onuma, M., & Ohashi, K.
  • Journal Title: Microbial. Immunol Volume: 53 Pages: 224-232
  • Peer Reviewed
• [Journal Article] Microarray analysis of host immune responses to Marek' s disease virus infection in vaccinated chickens (2009)
  • Author(s): Kano, R., Konnai, S., Onuma, M., & Ohashi, K.
  • Journal Title: J. Vet. fled. Sci Volume: 71 Pages: 603-610
  • Peer Reviewed
• [Journal Article] Cloning and characterization of Rhipicephalus appendiculatus voraxinalpha and its effect as anti-tick vaccine (2009)
  • Author(s): Yamada, S., Konnai, S., Imamura, S., Ito, T., Onuma, M., & Ohashi, K.
  • Journal Title: Vaccine Volume: 27 Pages: 5989-5997
  • Peer Reviewed
• [Journal Article] Comparative moleculo-immunological analysis of swamp and riverine type water buffaloes responses (2009)
  • Author(s): Mingala, C. N., Konnai, S., Cruz, L. C., Onuma, M., & Ohashi, K.
  • Journal Title: Cytokine Volume: 46 Pages: 273-282
  • Peer Reviewed
• [Journal Article] Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis (2009)
  • Author(s): Imamura, S., da Silva Vaz, I. Jr., Konnai, S., Yamada, S., Najajima, C., Onuma, M., Ohashi, K.
  • Journal Title: Exp. Appl. Acarol Volume: 48 Pages: 345-358
  • Peer Reviewed
• [Journal Article] Quantification of water buffalo (Bubalus bubalis) cytokine expression in response to inactivated foot-and-mouth disease (FMD) vaccine (2009)
  • Author(s): Mingala, C. N., Konnai, S., Venturina, A., Onuma, M., Ohashi, K.
  • Journal Title: Res. Vet. Sci Volume: 87 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Classification of new BVDV isolates from Philippine water buffalo using the viral E2 region J (2009)
  • Author(s): Mingala, C. N., Konnai, S., Onuma, M., Ohashi, K.
  • Journal Title: Basic illicrobiol Volume: 49 Pages: 495-500
  • Peer Reviewed
• [Journal Article] Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6 (2009)
  • Author(s): Mekata, H., Konnai, S., Witola, W. H., Inoue, N., Onuma, M., and Ohashi, K.
  • Journal Title: Infect. Genet. Evol Volume: 9 Pages: 1301-1305
  • Peer Reviewed
• [Journal Article] Microarray analysis of host immune responses to Marek's disease virus infection in vaccinated chickens. (2009)
  • Author(s): Kano, R., et al.
  • Journal Title: Journal of Veterinary Medical Science 71 Pages: 603-610
  • Peer Reviewed
• [Presentation] マレック病ウイルス強毒株由来L-Meqによるbcl-2遺伝子発現調節の比較 (2011)
  • Author(s): 村田史郎、伊勢崎政美、松山あゆ美、高崎紗蘭、今内覚、大橋和彦
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(堺)
  • Year and Date: 2011-09-20
• [Presentation] マレック病ウイルス強毒株由来Meqおよび弱毒株由来L-Meqによるbcl-2遺伝子発現調節の比較 (2011)
  • Author(s): 村田史郎
  • Organizer: 日本獣医学会
  • Place of Presentation: 大阪府立大学(大阪府)
  • Year and Date: 2011-09-20
• [Presentation] 野鳥由来感染症の分子診断法の開発および疫学調査 (2011)
  • Author(s): 大橋和彦、村田史郎、伊勢崎政美、今内覚
  • Organizer: 第53回日本生態学会学術集会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-03-08
• [Presentation] マガンおよび鶏におけるinterferon-γ遺伝子プロモーター活性の比較解析 (2010)
  • Author(s): 松原綾子、村田史郎、加納里佳、橋口知幸、伊勢崎政美、河東あゆ美、小沼操、今内覚、大橋和彦
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広)
  • Year and Date: 2010-09-18
• [Presentation] マガンおよび鶏におけるinterferon-γ遺伝子プロモーター活性の比較解析 (2010)
  • Author(s): 村田史郎, ら
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広市・帯広畜産大学
  • Year and Date: 2010-09-18
• [Presentation] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布 (2010)
  • Author(s): 大橋和彦、松原綾子、村田史郎、今内覚
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学(武蔵野)
  • Year and Date: 2010-03-28
• [Presentation] Regulation of the interferon gamma expression by an onocoprotein of Marek' s disease virus in wild waterfowls and chickens (2010)
  • Author(s): Matsubara, A., Murata, S., Kano, R., Hashiguchi, T., Isezaki, M., Kato, A., Onuma, M., Konnai, S., & Ohashi, K
  • Organizer: 9th International Veterinary Immunology symposium
  • Place of Presentation: Tokyo, Japan
• [Presentation] 弱毒および強毒マレック病ウイルス感染鶏における免疫応答に関する研究 (2009)
  • Author(s): 加納里佳、今内覚、小沼操、大橋和彦
  • Organizer: 第7回サイトカイン研究会学術集会
  • Place of Presentation: とりぎん文化会館(鳥取)
  • Year and Date: 2009-09-24
• [Presentation] 弱毒および強毒マレック病ウイルス感染鶏における免疫応答に関する研究 (2009)
  • Author(s): 大橋和彦, ら
  • Organizer: 第148回日本獣医学会学術集会(動物サイトカイン研究会学術集会)
  • Place of Presentation: 鳥取市・とりぎん文化会館
  • Year and Date: 2009-09-24