| Project/Area Number |
21500400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KONDO Eiji 北海道大学, 大学院・医学研究科, 講師 (60374724)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Kazunori 北海道大学, 大学院・医学研究科, 教授 (20166507)
ONODERA Shin 北海道大学, 大学院・医学研究科, 客員准教授 (00359481)
KITAMURA Nobuto 北海道大学, 大学院・医学研究科, 講師 (80447044)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生体制御 / 治療 / 靱帯 / 腱組織 / 液性因子 |
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| Research Abstract |
Background : It is well known that the structural properties of a tendon autograft deteriorate during the remodeling phase after anterior cruciate ligament (ACL) reconstruction.
Hypothesis : A local application of autologous synovium-derived cells cultured in TGF-betal-supplemented medium may inhibit the deterioration of structural properties of the tendon graft after ACL reconstruction.
Study Design : Controlled laboratory study
Methods : Fifty-two mature sheep were used in this study. In each animal, the right knee underwent ACL reconstruction using the semitendinosus tendon autograft. Then, the animals were divided into 5 groups of 10 sheep each. No additional treatments were applied in Group 1, while fibrin sealant was applied around the graft in Group 2. In Group 3, autologous synovium-derived cells cultured in standard medium were applied around the graft with fibrin sealant, while autologous synovium-derived cells cultured in TGF-betal-supplemented medium were applied with fibrin s
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ealant in Group 4. In Group 5, fibrin sealant containing 20-ng TGF-betal was applied around the graft. Each animal was sacrificed at 12 weeks after the surgery. In each group, 7 and 3 sheep were used for biomechanical and histological evaluations, respectively. The remaining two sheep were used to confirm whether the applied cells infiltrated into the graft at 1 week after surgery.
Results : Confocal microscope observations showed that the applied cells that were labeled before implantation infiltrated into the superficial portion of the graft at 1 week. Biomechanically, the maximum load and the stiffness of Groups 4 and 5 were significantly greater than those of Groups 1, 2, and 3. Histologically, necrotic lesions were observed in the core portion of the midsubstance in Groups 1 and 2. In Groups 3, 4, and 5, we could not find any necrotic lesions in the midsubstance. Conclusions : A local application of autologous synovium-derived cells cultured in TGF-beta1-supplemented medium, or fibrin sealant containing TGF-betal, significantly inhibit the natural deterioration of the structural properties of the tendon graft after ACL reconstruction. Less
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