| Project/Area Number |
21510063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
FUJIMOTO Hirofumi 国立感染症研究所, 放射能管理室, 主任研究官 (60373396)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Manabu 独立行政法人放射線医学総合研究所, 放射線障害研究グループ, 主任研究員 (70280740)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKUDOH Takashi 国立感染症研究所, 放射能管理室, 主任研究官 (70455393)
TSUCHIDA Kozo 国立感染症研究所, 放射能管理室, 室長 (40231435)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | DNA二本鎖切断 / Ku / DNA損傷認識 / 分子動力学シミュレーション / Kuタンパク質 |
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| Research Abstract |
The binding mechanism of the repair enzyme, Ku, to the end of double strand DNA was examined by both computational and experimental techniques. Molecular models of Ku-DNA complex were constructed to estimate the binding energy between Ku and DNA, and amino acids that would interact with both DNA and Ku itself were identified. The new Ku-deficient cell line was established to investigate a detailed function of the predicted region.
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