Basic study on the effect of endocrine disruptors on reptiles reproduction using a model of American alligators, Alligator Mississippiensis

• Project/Area Number: 21510068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Tottori University
• Principal Investigator: OHTA Yasuhiko 鳥取大学, 農学部・獣医学科, 教授 (60069078)
• Co-Investigator(Kenkyū-buntansha): KATSU Yoshinao 北海道大学, 大学院・理学研究院, 准教授 (00332180)
• Co-Investigator(Renkei-kenkyūsha): TAKEUCHI Takashi 鳥取大学, 農学部・獣医学科, 准教授 (10325061) ; IGUCHI Taisen 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 環境ホルモン / ハ虫類 / アメリカンアリゲーター / 性分化 / 生殖輸管 / 生殖輸管分化 / 生殖輸管系分化

Research Abstract

In American alligator showing temperature-dependent sex determination(TSD), development of reproductive organs, ontogeny of expression of estrogen and progesteronereceptor and candidates involved in the TSD were identified during the thermo-sensitiveperiod. These results may contribute to the understanding of endocrine disrupters onreptiles.

Research Products

• [Journal Article] Altered gonadal expression of TGFαsuperfamily signaling factors in environmental contaminant-exposed juvenile alligators (2011)
  • Author(s): Moore, B. C., Milnes, M. R., Kohno, S., Katsu, Y., Iguchi, T., Woodruff, T. K., Guillette, L, Jr.
  • Journal Title: J. Steroid Biochem. Mol. Biol. Volume: 127 Issue: 1-2 Pages: 58-63
  • DOI: 10.1016/j.jsbmb.2011.01.004
  • Peer Reviewed
• [Journal Article] Sex steroid and thyroid hormone receptor expressions in the thyroid of the American alligator(Alligator mississippiensis) during different life stages (2011)
  • Author(s): Bermudez, D., Skotko, J. P., Ohta, Y. Boggs, S. S. P., Iguchi, T., Guillette, L. J, Jr.
  • Journal Title: J. Morphology Volume: 271 Issue: 6 Pages: 698-703
  • DOI: 10.1002/jmor.10936
  • Peer Reviewed
• [Journal Article] Molecularcloning pf anti-Mullerian hormone from the American alligator, Alligator mississippiensi (2011)
  • Author(s): Urushitani, H., katsu, Y., Miyagawa, S., Kohno, S., Ohta, Y., Guillette, L. J. Jr., Iguchi, T.
  • Journal Title: Mol. Cel. Endcrinol. Volume: 333 Issue: 2 Pages: 190-199
  • DOI: 10.1016/j.mce.2010.12.025
  • Peer Reviewed
• [Journal Article] Molecular cloning characterization, and chromosome mapping of reptilian estrogen receptors (2010)
  • Author(s): Katsu, Y., Matsubara, K., Kohno, S., Toriba, M., Oka, K., Guillette, L. J, Jr., Ohta, Y., Iguchi, T.
  • Journal Title: Endocrinology Volume: 151 Issue: 12 Pages: 5710-5720
  • DOI: 10.1210/en.2010-0356
  • Peer Reviewed
• [Journal Article] Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator (2010)
  • Author(s): Kohno, S., Katsu, Y., Urushitani, U., Ohta, Y., Iguchi, T., Guillette, L. Jr.
  • Journal Title: Sexual Devel. Volume: 4 Issue: 1-2 Pages: 73-87
  • DOI: 10.1159/000260374
  • Peer Reviewed
• [Journal Article] Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator (2010)
  • Author(s): S.Kohno, Y.Katsu, H.Urushitani, Y.Ohta, T.Iguchi, L.J.Guillette, Jr.
  • Journal Title: Sexual Development 4 Pages: 73-87
  • Peer Reviewed
• [Journal Article] Molecular cloning pf anti-Mullerian hormone from the American alligator, Alligator mississippiensis
  • Author(s): Urushitani, H., katsu, Y., Miyagawa, S., Kohno, S., Ohta, Y., Guillette, L.J, Jr., Iguchi, T.
  • Journal Title: Mol.Col.Endcrinol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Sex steroid and thyroid hormone receptor expressions in the thyroid of the American alligator (Alligator mississippiensis) during different life stages
  • Author(s): Bermudez, D., Skotko, J.P., Ohta, Y., Boggs, S.S.P., Iguchi, T., Guillette, L.J, Jr.
  • Journal Title: J.Morphology Volume: (in press)
  • Peer Reviewed
• [Presentation] アメリカンアリゲーターのコルチコイド受容体の単離と機能解析 (2011)
  • Author(s): 岡香織・太田康彦・井口泰泉・勝義直
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-11
• [Presentation] アメリカアリゲーターのコルチコイド受容体の単離と機能解析 (2011)
  • Author(s): 岡香織・太田康彦・井口泰泉・勝義直
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(旭川市)
  • Year and Date: 2011-09-11
• [Presentation] Which estrogen receptor(ESR1 ; ERαor EXR2 ; ERβ) is involved in sex determination and gonadal differentiation? Michigan University (2011)
  • Author(s): Kohno, S. Katus, Y. Ohta, Y. Igutchi, T., Guillette, L. J.
  • Organizer: NASCE2011
  • Place of Presentation: Michigan league(Ann Arbor)
  • Year and Date: 2011-07-14
• [Presentation] Which estrogen receptor (ESR1 ; ERα or EXR2 ; ERβ) is involved in sex determination and gonadal differentiation? (2011)
  • Author(s): Kohno, S.Katus, Y.Ohta, Y.Igutchi, t., Guillette, L.J.
  • Organizer: NASCE 2011
  • Place of Presentation: Michigan University Michigan league (Ann Arbor)
  • Year and Date: 2011-07-14
• [Presentation] A Preliminary Study on Immunohisitochemical Detection of Estrogen Receptor in American Alligator Oviducts-Specificity of Antibodies (2009)
  • Author(s): Ohta, Y., Urushidani, H., Takeuchi, T., Iguchi, T., Katsu, Y., Kohno, S., Brandon, M. and Guillette, L. J.
  • Place of Presentation: Tulene University(New Orleans)
  • Year and Date: 2009-10-21
• [Presentation] A Preliminary Study on Immunohisitochemical Detection of Estrogen Receptor in American Alligator Oviducts-Specificity of Antibodies (2009)
  • Author(s): Ohta, Y., Urushidani, H., Takeuchi, T., Iguchi, T., Katsu, Y., Kohno, S., Brandon, M., Guillette, L.J.
  • Organizer: e.hormone
  • Place of Presentation: New Orleans
  • Year and Date: 2009-10-21
• [Remarks]
  • URL: http://staff.muses.tottori-u.ac.jp/ohta