| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
One of Type IV secretion systems (T4SS), which is derived from an IncP type plasmid, was applied as a novel gene introduction method into eukaryotic cells. We found that mitochondrial respiratory mutants in S. cerevisiae showed enhanced transformation efficiency. By using inhibitors of mitochondrial function, we successfully established a simple and efficient gene introduction method into S. cerevisiae from E. coli. In addition, we firstly observed direct gene transfer from E.coli to higher plant cells by applying this T4SS.
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