| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
In this study, we extracted and purified the 14-kDa species of ChM-I from rat rib cartilage. Amino acid sequencing revealed a 14-kDa ChM-I lacks the N-terminal 37 residues of mature 25-kDa ChM-I. As demonstrated in vitro, 14-kDa ChM-I exhibited little anti-angiogenic activity. There is no 25-kDa ChM-I species present in the hypertrophic/calcified cartilage zone of cartilaginous bone precursors. Instead only the 14-kDa ChM-I species was detected. Taken together, our data suggest that ChM-I is inactivated by its N-terminal deletion of 37 residues during endochondral bone formation.
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