Synthesis of Cationic Glycopolymer and Development of Sugar-Coated Polyplex for Gene Delivery Application

• Project/Area Number: 21550156
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: University of Yamanashi
• Principal Investigator: OBATA Makoto 山梨大学, 大学院・医学工学総合研究部, 准教授 (70343267)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ラジカル重合 / RAFT重合 / 糖質高分子 / 核酸キャリア

Research Abstract

We have performed the synthesis of novel cationic glycopolymer, which is synthetic polymer bearing carbohydrate moieties, and the evaluation of their functions as a gene carrier. Block copolymers of cationic monomer with vinyl monomer bearing D-mannose have been successfully synthesized by means of precisely controlled polymerization. Preliminary in vitro experiment afforded a key for the molecular architecture of the efficient cationic glycopolymer as gene carrier.

Research Products

• [Journal Article] Current states and future views in photodynamic therapy (2011)
  • Author(s): Shigenobu Yano, Shiho Hirohara, Makoto Obata, Yuichiro Hagiya, Shun-ichiro Ogura, Atsushi Ikeda, Hiromi Kataoka, Mamoru Tanaka, Takashi Joh
  • Journal Title: J. Photochem. Photobiol. C, Photochem. Rev. Volume: 12 Pages: 46-67
  • NAID: 10029471944
  • Peer Reviewed
• [Journal Article] Characterization and Cellular Internalization of Poly(2-hydroxyethyl methacrylate) bearing.-D-Mannopyranose (2011)
  • Author(s): Makoto Obata, Mayumi Shimizu, Tomoko Ohta, Aya Matsushige, Kaoru Iwai, Shiho Hirohara, Masao Tanihara, Synthesis
  • Journal Title: Polym. Chem. Volume: 2 Pages: 651-658
  • Peer Reviewed
• [Journal Article] Syntheses of 3-Arm and 4-Arm Star-Branched Polystyrene Ru(II) Complexes by the Click-to-Chelate Approach (2011)
  • Author(s): Chunhong Zhang, Xiande Shen, Ryosuke Sakai, Michael Gottschaldt, Ulrich S. Schubert, Shiho Hirohara, Masao Tanihara, Shigenobu Yano, Makoto Obata, Nao Xiao, Toshifumi Satoh, Toyoji Kakuchi
  • Journal Title: J. Polym. Sci. Part A : Polym. Chem. Volume: 49 Pages: 746-753
  • Peer Reviewed
• [Journal Article] Current states and future views in photodynamic therapy (2011)
  • Author(s): S.Yano, S.Hirohara, M.Obata, Y.Hagiya, S.Ogura, A.Ikeda, H. Kataoka, M.Tanaka, T.Joh
  • Journal Title: Journal of Photochemistry and Photobiology C : Photochemistry Reviews Volume: 12 Issue: 1 Pages: 46-67
  • DOI: 10.1016/j.jphotochemrev.2011.06.001
  • NAID: 10029471944
  • Peer Reviewed
• [Journal Article] Synthesis, Characterization and Cellular Internalization of Poly(2-hydroxyethyl methacrylate bearing α-D-Mannopyranose (2011)
  • Author(s): Makoto Obata
  • Journal Title: Polymer Chemistry Volume: 2 Pages: 651-658
  • Peer Reviewed
• [Journal Article] Syntheses of 3-arm and 4-Arm Star-Branched Polystyrene Ru(II) Complexes by the Click-to-Chelate Approach (2011)
  • Author(s): Chunhong Zhang
  • Journal Title: Journal of Polymer Science Part A : Polymer Chemistry Volume: 49 Pages: 746-753
  • Peer Reviewed
• [Journal Article] Michael Gottschaldt, Aram Prokop, Mitochondrial mode of action of a thymidine-based cisplatin analog breaks resistance in cancer cells (2010)
  • Author(s): Liliane A. Onambele, Daniel Koth, Justyna A. Czaplewska, Ulrich S. Schubert, Helmar Gorls, Shigenobu Yano, Makoto Obata
  • Journal Title: Chem. Eur. J. Volume: 16 Pages: 14498-14505
  • Peer Reviewed
• [Journal Article] Plasmon-Enhanced Luminescence from Ultrathin Hybrid Polymer Nanoassemblies for Microscopic Oxygen Sensor Application (2010)
  • Author(s): Masaya Mitsuishi, Hiroyuki Tanaka, Makoto Obata, Tokuji Miyashita
  • Journal Title: Langmuir Volume: 26 Pages: 15117-15120
  • Peer Reviewed
• [Journal Article] Synthesis, Photophysical Properties and Photocytotoxicity of Mono-, Di-, Tri-and Tetra-Glucosylated Fluorophyenylporphyrins (2010)
  • Author(s): Shiho Hirohara, Masataka Nishida, Kohei Sharyo, Makoto Obata, Tsuyoshi Ando, Masao Tanihara
  • Journal Title: Bioorg. Med. Chem. Volume: 18 Pages: 1526-1535
  • Peer Reviewed
• [Journal Article] Oxygen-Sensing Properties of 5, 10, 15, 20-Tetraphenylporphinato Platinum(II) and Palladium(II) Covalently Bound on Poly(isobutyl-co-2, 2, 2-trifluoroethyl methacrylate (2010)
  • Author(s): Makoto Obata, Noriko Matsuura, Kazunori Mitsuo, Hiroki Nagai, Keisuke Asai, Masafumi Harada, Shiho Hirohara, Masao Tanihara, Shigenobu Yano
  • Journal Title: J. Polym. Sci. Part A : Polym. Chem. Volume: 48 Pages: 663-670
  • Peer Reviewed
• [Journal Article] Mitochondrial mode of action of a thymidine-based cisplatin analog breaks resistance in cancer cells (2010)
  • Author(s): Liliane A.Onambele
  • Journal Title: Chemistry A European Journal Volume: 16 Pages: 14498-14505
  • Peer Reviewed
• [Journal Article] Synthesis, Photophysical Properties and Photocytotoxicity of Mono-, Di-, Tri-, and Tetra-Glucosylated Fluorophyenylporphyrins (2010)
  • Author(s): Shiho Hirohara
  • Journal Title: Bioorganic & Medicinal Chemistry 18 Pages: 1526-1535
  • Peer Reviewed
• [Journal Article] Oxygen-Sensing Properties of 5, 10, 15, 20-Tetraphenylporphinato Platinum(II) and Palladium(II) Covalently Bound on Poly(isobutyl-co-2, 2, 2-trifluoroethyl methacrylate) (2010)
  • Author(s): Makoto Obata
  • Journal Title: Journal of Polymer Science Part A : Polymer Chemistry 48 Pages: 663-670
  • Peer Reviewed
• [Journal Article] Antioxidant action of sugar-pendant C60 fullerenes (2009)
  • Author(s): Masanori Horie, Akiko Fukuhara, Yoshiro Saito, Yasukazu Yoshida, Hiroe Sato, Hiromi Ohi, Makoto Obata, Yuji Mikata, Shigenobu Yano, Etsuo Niki
  • Journal Title: Bioorg Med. Chem. Lett. Volume: 19 Pages: 5902-5904
  • Peer Reviewed
• [Journal Article] Synthesis, photophysical properties and sugar-dependent in vitro photocytotoxicity of pyrrolidine-fused chlorins bearing S-glycosides (2009)
  • Author(s): Shiho Hirohara, Makoto Obata, Hiroki Alitomo, Kohei Sharyo, Tsuyoshi Ando, Masao Tanihara, Shigenobu Yano
  • Journal Title: J. Photochem. Photobiol. B, Biol. Volume: 97 Pages: 22-33
  • Peer Reviewed
• [Journal Article] EXAFS Study on Reaction of Anti-tumor Platinum Complexes with Reduced Glutathione (2009)
  • Author(s): Makoto Obata, Masafumi Harada, Hiromi Ohi, Shiho Hirohara, Michael Gottchaldt, Shigenobu Yano
  • Journal Title: Chem. Pharm. Bull. Volume: 57 Pages: 1107-1109
  • Peer Reviewed
• [Presentation] カチオン性セグメントを有する糖質高分子の合成とポリプレックス形成 (2012)
  • Author(s): 小幡誠,小堀智也
  • Organizer: 第92回日本化学会春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス・矢上キャンパス
  • Year and Date: 2012-03-27
• [Presentation] カチオン性セグメントを有する糖質高分子の合成とポリプレックス形成 (2012)
  • Author(s): 小幡誠、小堀智也
  • Organizer: 第92回日本化学会春季年会
  • Place of Presentation: 慶應義塾大学(横浜市)
  • Year and Date: 2012-03-27
• [Presentation] マンノースを側鎖に有するカチオン性糖質高分子の合成とその性質 (2011)
  • Author(s): 小幡誠,小堀智也
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2011-09-30
• [Presentation] カチオン性糖質高分子の合成とポリプレックス形成能 (2011)
  • Author(s): 小幡誠、小堀智也
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2011-09-28
• [Presentation] マンノピラノシル基を有するメタクリル酸エステルの合成と重合およびその細胞内取り込み (2011)
  • Author(s): 小幡誠,太田智子,廣原志保,谷原正夫
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2011-09-12
• [Presentation] マンノピラノシル基を有するメタクリル酸エステルの合成と重合およびその細胞内取り込み (2011)
  • Author(s): 小幡誠, 他3名
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場(つくば市)
  • Year and Date: 2011-09-12
• [Presentation] マンノースを側鎖に有する糖質高分子の合成と性質 (2010)
  • Author(s): 小幡誠,清水真弓,岩井薫,廣原志保,谷原正夫
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2010-09-26
• [Presentation] マンノースを側鎖に有する糖質高分子の合成と性質 (2010)
  • Author(s): 小幡誠, 他4名
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2010-09-24
• [Presentation] メタクリル酸2-(-D-マンノピラノシルオキシ)エチルとメタクリル酸ジメチルアミノエチルのラジカル共重合 (2010)
  • Author(s): 小堀智也,小幡誠
  • Organizer: 第59回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-05-26
• [Presentation] メタクリル酸2-(-D-マンノピラノシルオキシ)エチルとメタクリル酸2-ヒドロキシエチルのラジカル共重合 (2010)
  • Author(s): 清水真弓,岩井薫,小幡誠
  • Organizer: 第59回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-05-26
• [Presentation] メタクリル酸マンノピラノシルオキシエチルとメタクリル酸ジメチルアミノエチルのラジカル共重合 (2010)
  • Author(s): 小幡誠
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学,大阪
  • Year and Date: 2010-03-28
• [Presentation] メタクリル酸マンノピラノシルオキシエチルとメタクリル酸ジメチルアミノエチルのラジカル共重合 (2010)
  • Author(s): 小幡誠
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学(大阪)
  • Year and Date: 2010-03-28
• [Remarks]
  • URL: http://www.ccn.yamanashi.ac.jp/~mobata/index.html
• [Remarks]
  • URL: http://www.ccn.yamanashi.ac.jp/~mobata/index.html