Development of alkyl curdlan sulfate-coated nano fiber with specific influenza A virus-adsorptive functionality
| Project/Area Number |
21550199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kitami Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Hideki 聖マリアンナ医科大学, 医学部, 教授 (20192669)
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| Co-Investigator(Renkei-kenkyūsha) |
URYU Toshiyuki (80011005)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | カードラン硫酸 / アルキル鎖導入カードラン硫酸 / インフルエンザウイルス / メンブランフィルター / 静電的相互作用 / HA価 / インフルエンザ / アルキル鎖 / 疎水的相互作用 / 抗ウイルス性 / 吸着 |
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| Research Abstract |
To develop a biomaterial with an influenza virus-adsorptive functionality, an alkyl curdlan sulfate was prepared by ionic interaction between a positively charged didodecyldimethyl ammonium bromide and a negatively charged sulfate group of curdlan sulfate, which has potent anti-HIV activity, and then coated on a membrane filter with a 1-μm pore size by hydrophobic interaction with the long alkyl groups in the curdlan sulfate. The alkyl curdlan sulfate with the degree of alkylation (DOA) of 0.03 (1 didodecyldimethyl group/12 sugar residues with 36 hydroxyl or sulfate groups) showed potent anti-HIV activity in a 50% effective concentration (EC_<50>) as low as 0.87μg/ml (standard curdlan sulfate EC_<50>=0.3μg/ml). When 1.6 mg of the alkyl curdlan sulfate with the DOA of 0.03 was coated on a membrane filter (13 mmΦ, pore size : 1μm), three stacked alkyl curdlan sulfate-coated membrane filters dramatically decreased hemagglutination to 1/4-1/32. These results can therefore be presumed to demonstrate that the alkyl curdlan sulfate-coated membrane filter removed influenza A viruses by adsorption between the negatively charged sulfate groups and the positively charged envelope protein of the virus.
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Report
(4 results)
Research Products
(110 results)
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[Journal Article] Effects of lacquer polysaccharides, glycoproteins and isoenzymes on the activity of free and immobilised laccase from Rhus vernicifera2010
Author(s)
Y. Wan, R. Lu, K. Akiyama, K. Okamoto, T. Honda, Y. Du, T. Yoshida, T. Miyakoshi, C. J. Knill, J. F. Kennedy
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Journal Title
Internal. J. Biol. l Macromol
Volume: 47
Pages: 76-81
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[Presentation] 漆糖鎖の分離と構造2012
Author(s)
白玉廷、石村敬久、吉田孝
Organizer
第46回高分子学会北海道支部研究発表会
Place of Presentation
札幌
Year and Date
2012-01-31
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