Development of the effective novel identification method of the proteins which accumulate uranium
| Project/Area Number |
21560878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nuclear engineering
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
SAKAMOTO Fuminori 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 研究副主幹 (60391273)
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| Co-Investigator(Kenkyū-buntansha) |
KOZAI Naofumi 独立行政法人日本原子力研究開発機構, 端基礎研究センター, 研究主幹 (80354877)
鈴木 義規 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 博士研究員 (20455281)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 核燃料サイクル / 酵母 / ウラン濃集 / タンパク質 / ウラン濃集タンパク質 / 非含硫アミノ酸 |
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| Research Abstract |
We developed new technique to identify the protein which specifically accumulated uranium effectively. This method uses the detection system that combined mass spectrograph with liquid chromatography using a size exclusion column. We tried to identify the proteins which accumulate uranium using this method. As a result, we made clear that about 46 kilo Daltons of protein accumulated uranium. It was also suggested that the protein did not conclude the amino acid including sulfur(methionine and cysteine).
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Report
(4 results)
Research Products
(28 results)
