Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions

• Project/Area Number: 21570034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: NISHIDA Ikuo 埼玉大学, 大学院・理工学研究科, 教授 (40189288)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ホスファチジルコリン / ホスファチジルエタノールアミン / 低温応答 / リン脂質合成 / 根の伸長 / 表皮細胞の内膜構造 / CDP-コリン合成 / ホスファチジルセリン / リン脂質合成のしくみ / 根の表皮細胞 / 内膜構造

Research Abstract

WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.

Research Products

• [Journal Article] PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana (2011)
  • Author(s): Yamaoka Y., Yu Y., Mizoi J., Fujiki Y., Saito K., Nishijima M., Lee Y., Nishida I.
  • Journal Title: Plant J Volume: 67 Issue: 4 Pages: 648-661
  • DOI: 10.1111/j.1365-313x.2011.04624.x
  • Peer Reviewed
• [Journal Article] PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana (2011)
  • Author(s): Yamaoka, Y., Yu, Y., Mizoi, J., Fujiki, Y., Saito, K., Nishijima, M., Lee, Y., Nishida, I.
  • Journal Title: The Plant Journal Volume: In press
  • Peer Reviewed
• [Journal Article] Isozyme-specifc modes of activation of CTP : phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature (2009)
  • Author(s): Inatsugi R., Kawai H., Yamaoka Y., Yu Y., Sekiguchi A., Nakamura M., Nishida I.
  • Journal Title: Plant Cell Physiol Volume: 50 Issue: 10 Pages: 1727-1735
  • DOI: 10.1093/pcp/pcp115
  • Peer Reviewed
• [Journal Article] Isozyme-specifc modes of activation of CTP : phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature (2009)
  • Author(s): Inatsugi, R., Kawai, H., Yamaoka, Y., Yu, Y., Sekiguchi, A., Nakamura, M., Nishida, I.
  • Journal Title: Plant Cell Physiol 50 Pages: 1727-1735
  • Peer Reviewed
• [Presentation] pect1-4 mutation induces an alteration in PE/PC ratio and affects the cytochrome pathway capacity of mitochondrial respiration (2012)
  • Author(s): Otsuru, M., Yu, Y., Mizoi, J., Fujiki, Y., Nishida, I.
  • Organizer: 53th JSPP Annual Meeting
  • Place of Presentation: Kyoto Sangyo University
  • Year and Date: 2012-03-16
• [Presentation] The effects of pect1-4 mutation on mitochondrial lipid composition and respiration capacity in Arabidopsis at low temperature (2011)
  • Author(s): Yu Y., Otsuru M., Duan Z., Fujiki Y., Nishida I.
  • Organizer: The Fourth Asian Symposium on Plant Lipids
  • Place of Presentation: Hong Kong
  • Year and Date: 2011-12-02
• [Presentation] Tpect1-4 mutation decreases leaf respiration byaffecting the mitochondrial cytochrome pathway capacity (2011)
  • Author(s): Otsuru M., Yu Y., Mizoi J., Fujiki Y., Nishida I.
  • Organizer: The Fourth Asian Symposium on Plant Lipids
  • Place of Presentation: Hong Kong
  • Year and Date: 2011-12-02
• [Presentation] The importance of PHOSPHATIDYLSERINE SYNTASE1 in root development of Arabidopsis thaliana (2011)
  • Author(s): Yamaoka Y., Duan Z., Fujiki Y., Nishida I.
  • Organizer: The Fourth Asian Symposium on Plant Lipids
  • Place of Presentation: Hong Kong
  • Year and Date: 2011-12-02
• [Presentation] The effects of pect1-4 mutation on mitochondrial lipid composition and respiration capacity in Arabidopsis at low temperature (2011)
  • Author(s): Yu, Y, Otsuru, M., Duan, Z., Fujiki, Y., Nishida, I.
  • Organizer: The Fourth Asian Symposium on Plant Lipids
  • Place of Presentation: The University of Hong Kong
  • Year and Date: 2011-12-02
• [Presentation] シロイヌナズナの根におけるホスファチジルセリンの役割の解明 (2011)
  • Author(s): 山岡靖代, 溝井順哉, 藤木友紀, 西田生郎
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
• [Presentation] シロイヌナズナにおけるホスファチジルセリン生合成の分子機構と花粉成熟における役割の解明 (2011)
  • Author(s): 山岡靖代, 溝井順哉, 藤木友紀, 西田生郎
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] Physiological Significance of CTP : Phosphorylcholine Cytidylytransferase Genes in Arabidopsis Roots under Stress Conditions (2010)
  • Author(s): Nishida, I., Mori, Y., Tsukano, Y., Yu, Y., Kawai, H., Yamaoka, Y, Lee, Y., Fujiki, Y.
  • Organizer: 19th International Symposium on Plant Lipids
  • Place of Presentation: Cairns Australia
  • Year and Date: 2010-07-11
• [Presentation] Physiological Significance of CTP : phosphorylcholine cytidylytransferase genes in Arabidopsis Roots under Stress Conditions (2010)
  • Author(s): Nishida I., Mori Y., Tsukano Y., Yu Y., Kawai H., Yamaoka Y., Lee Y., Fujiki Y.
  • Organizer: 19th International Symposium on Plant Lipids
  • Place of Presentation: Cairns
  • Year and Date: 2010-07-10
• [Presentation] Unusual phenotypes of Arabidopsis mutants defective in phospholipid biosynthesis (2010)
  • Author(s): Nishida, I
  • Organizer: 2010 KSBMB Annual Meeting
  • Place of Presentation: Seoul
• [Presentation] シロイヌナズナのCDP-コリン合成酵素欠損株における花のホメオティック変異とBクラス陰電子のメチル化との関係 (2010)
  • Author(s): 早川慶紀, Jun-Young Jin, 関口陽, 溝井順哉, 藤木友紀, Youngsook Lee, 西田生郎
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] The effects of pect1-4mutation on the lipid composition and the respiration activity of isolated mitochondria (2010)
  • Author(s): Yu, Y., Kawai, H., Kawamoto, M., Mizoi, J., Fujiki, Y., Nishida, I.
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
• [Presentation] CTP : phosphorylcholine cytidylyltransferase genes are important for endomembrane development in Arabidopsis thaliana root cells (2009)
  • Author(s): Nishida I., Tsukano J., Mori Y., Yamaoka Y., Yu Y., Kawai H., Fujiki Y.
  • Organizer: The Third Asian Symposium on Plant Lipids
  • Place of Presentation: Yokohama
  • Year and Date: 2009-11-28
• [Presentation] CTP : phosphorylcholine cytidylyltransferase genes are important for endomembrane development in Arabidopsis thaliana root cells (2009)
  • Author(s): Nishida, I., Tsukano, J., Mori, Y., Yamaoka, Y., Yu, Y., Kawai, H., Fujiki, Y.
  • Organizer: The Third Asian Symposium on Platn Lipids
  • Place of Presentation: Yokohama Media and Communications Center
  • Year and Date: 2009-11-28
• [Remarks] (1)低温でリン脂質ホスファチジルコリンが生合成されるしくみ
  • URL: http://www.molbiol.saitama-u.ac.jp/~nishida/Nishida_Lab/Pub53.html
• [Remarks] (2)花粉成熟におけるホスファチジルセリン生合成の意義に関する研究
  • URL: http://www.molbiol.saitama-u.ac.jp/~nishida/Nishida_Lab/Pub54.html