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Analysis of the plastid-derived signal transduction that regulate nuclear gene expression

Research Project

Project/Area Number 21570039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

MOCHIZUKI Nobuyoshi  京都大学, 大学院・理学研究科, 助教 (60280939)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords葉緑体 / オルガネラ / レトログレードシグナル / テトラピロール / ABA / 葉緑体シグナル / 遺伝子発現 / シグナル伝達
Research Abstract

In higher plants, it has been hypothesised that there are unknown signals (“plastid signals”) derived from plastids to control nuclear gene expression. Analysis of gun mutants that have defects in this signal transduction pathway suggests the involvement of the tetrapyrrole intermediates (e.g. Mg-Protoporphyrin IX, MgProto) as signaling factors. Although the involvement of tetrapyrrole biosynthesis has been strongly suggested, the entity of the signal is still obscure. For better understanding of plastid signaling, we have been screening the gain-of-function type gun mutants using FOX-hunting (Riken Arabidopsis Full Length cDNA overexpressor) lines. We identified genes that encode a member of bHLH gene family and a kelch-repeat containing factor.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Disrupting the bimolecular binding of the haem-binding protein 5 (AtHBP5) to haem oxygenase 1 (HY1) leads to oxidative stress in Arabidopsis2012

    • Author(s)
      Lee HJ, Mochizuki N, Masuda T, Buckhout TJ
    • Journal Title

      J Exp Bot

      Volume: 63巻 Issue: 16 Pages: 5967-5978

    • DOI

      10.1093/jxb/ers242

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation ofunbound free heme in plant cells by differential acetone extraction2012

    • Author(s)
      Espinas NA, Kobayashi K, Takahashi S, Mochizuki N, Masuda T
    • Journal Title

      Plant Cell Physiol

      Volume: 53巻 Issue: 7 Pages: 1344-1354

    • DOI

      10.1093/pcp/pcs067

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcellular sites of the signal transduction and degradation of phytochrome A.2010

    • Author(s)
      Toledo-Ortiz G, Kiryu Y, Kobayashi J, Oka Y, Kim Y, Nam HG, Mochizuki N, Nagatani A.
    • Journal Title

      Plant Cell Physiol.

      Volume: 51 Pages: 1648-1660

    • NAID

      10027353109

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The cell biology of tetrapyrroles : a life and death struggle.2010

    • Author(s)
      Mochizuki N, Tanaka R, Grimm B, Masuda T, Moulin M, Smith AG, Tanaka A, Terry MJ.
    • Journal Title

      Trends Plant Sci.

      Volume: 15 Pages: 488-498

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] アラビドプシスにおける簡便な気孔観察を可能とする新規表皮剥離法2012

    • Author(s)
      衣幡春映、長谷あきら、望月伸悦
    • Organizer
      第76回植物学会大会
    • Place of Presentation
      兵庫県立大学(兵庫県)
    • Related Report
      2011 Final Research Report
  • [Presentation] アラビドプシスにおける簡便な気孔観察を可能とする新規表皮剥離法2012

    • Author(s)
      衣幡春映
    • Organizer
      植物学会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 避陰応答による胚軸伸長促進におけるオーキシンの役割の時空間的解析2011

    • Author(s)
      小林淳子、綿引和巳、細川陽一郎、小塚俊明、望月伸悦、長谷あきら
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] フィトクロムAのN末端側領域の構造とphyAの特殊機能の関係2011

    • Author(s)
      吉川由希子、岡義人、望月伸悦、鈴木友美、長谷あきら
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナのフェロキラターゼイソフォームFC1およびFC2の比較解析2011

    • Author(s)
      Nino Espinasp、望月伸悦、増田建
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] プラスチドシグナル伝達におけるテトラピロール合成の関わり2010

    • Author(s)
      望月伸悦、吉積毅、近藤陽一、松井南、河内孝之、長谷あき, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Plastid-to-nucleus signaling.2009

    • Author(s)
      Nobuyoshi Mochizuki, Akira Nagatani
    • Organizer
      国際生物学賞記念シンポジウム
    • Place of Presentation
      京都大学 芝蘭会館(京都府)
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.bot.kyoto-u.ac.jp/j/1_seiri.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 植物学教室ー植物生理学

    • URL

      http://www.bot.kyoto-u.ac.jp/j/1_seiri.html

    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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