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Analysis of signaling factors related to stability of plant cortical microtubules

Research Project

Project/Area Number 21570043
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

KATO Takehide  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (70379535)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsオルガネラ / 細胞壁 / シロイヌナズナ / 微小管 / リン酸化 / フォスファターゼ / MAPキナーゼ / 細胞骨格 / チューブリン / 植物
Research Abstract

It was elucidated that the N-terminus conserved region of PHS1 showed the activities of dissipation of cortical microtubules in Arabidopsis. The region phosphorylated PHS1 oneself andα-tubulins, directly. Moreover the activities ware repressed by the C-terminus region of PHS1. Thus, these results indicated the possibility that the existence or dissipation of cortical microtubules may be controlled by PHS1 protein, which possesses both kinase domain and phosphatase domain. It was also shown that the PHS1-mediated phospholylation ofα-tubulins was induced by salt stress.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (14 results)

  • [Journal Article] Arabidopsis GCP3-INTERACTING PROTEIN 1/MOZART1 is an integral component of theγ-tubulin-containing microtubule nucleating complex. Plant J2012

    • Author(s)
      Nakamura M, Yagi N, Kato T, Fujita A, Kawashima N, Ehrhardt D. W. and Hashimoto T.
    • Journal Title

      Article first published online

      Volume: 14

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis GCP3-INTERACTING PROTEIN1/MOZAET1 is an integral component of the Y-tubulin-containing microtubule nucleating complex2012

    • Author(s)
      Nakamura M, Yagi N, Kato T, Fujita S, Kawashima N, Ehrhardt DW, Hashimoto T
    • Journal Title

      The Plant Journal

      Volume: (In Press) Issue: 2 Pages: 216-225

    • DOI

      10.1111/j.1365-313x.2012.04988.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms2010

    • Author(s)
      Pytela J, Kato T and Hashimoto T.
    • Journal Title

      Planta

      Volume: 231(6) Pages: 1311-22

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Defects in Dynamics and Functions of Actin Filament in Arabidopsis Caused by the Dominant-Negative Actin fiz1-Induced Fragmentation of Actin Filament2010

    • Author(s)
      Kato T, Morita MT and Tasaka M.
    • Journal Title

      Plant Cell Physiol

      Volume: 51(2) Pages: 333-8

    • NAID

      10027347163

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Defects in Dynamics and Functions of Actin Filament in Arabidopsis Caused by the Dominant-Negative Actin fizl-Induced Fragmentation of Actin Filament2010

    • Author(s)
      Takehide Kato, Miyo Terao Morita, Masao Tasaka
    • Journal Title

      Plant Cell Physiol. 51(2)

      Pages: 333-338

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Functional Analysis of Novelγ-Tubulin Ring Complex Proteins2012

    • Author(s)
      Noriyoshi Yagi, Masayoshi Nakamura, Noriyuki Kawashima, Takehide Kato, Takashi Hashimoto
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional Analysis of Novel γ-Tubulin Ring Complex Proteins2012

    • Author(s)
      Noriyoshi Yagi, Masayoshi Nakamura, Noriyuki Kawashima, Takehide Kato, Takashi Hashimoto
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2012-03-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Atypical kinase PHS1は微小管の脱重合を促進する2012

    • Author(s)
      藤田智史、Pytela Jaromir、野村有子、加藤壮英、高林周平、中神弘史、橋本隆
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Atypical kinase PHS1は微小管の脱重合を促進する2012

    • Author(s)
      藤田智史、Pytela Jaromir、野村有子、加藤壮英、高林周平、中神弘史、橋本隆
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] PROPYZAMIDE HYPERSENSITIVE2 is a novel microtubule associate protein related with plant cell elongation2011

    • Author(s)
      Takehide Kato, Kuniko Naoi, Takashi Hashimoto
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] An atypical kinase PHS1 induces microtubule destabilization in Arabidopsis2011

    • Author(s)
      Satoshi Fujita, Jaromir Pytela, Takehide Kato, Shuhei Takabayashi, Takashi Hashimoto
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] PROPYZAMIDE HYPERSENSITIVE2 is a novel microtubule associate protein related with plant cell elongation2011

    • Author(s)
      Takehide Kato, Kuniko Naoi, Takashi Hashimoto
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] An atypical kinase PHS1 induces microtubule destabilization in Arabidopsis2011

    • Author(s)
      Satoshi Fujita, Jaromir Pytela, Takehide Kato, Shuhei Takabayashi, Takashi Hashimoto
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞伸長に関与する新規微小管関連遺伝子PHS2の解析2011

    • Author(s)
      加藤壮英、橋本隆
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-21
    • Related Report
      2011 Final Research Report
  • [Presentation] PHS1の新奇キナーゼ様ドメインは微小管脱重合を促進する2011

    • Author(s)
      藤田智史、Pytela Jaromir、加藤壮英、乾良充、神戸雅人、橋本隆
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞伸長に関与する新規微小管関連遺伝子PHS2の解析2011

    • Author(s)
      加藤壮英、橋本隆
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      (仙台の予定が中止となり、3月11日付け年会講演要旨集のWeb公開をもって発表成立)
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] PHS1の新奇キナーゼ様ドメインは微小管脱重合を促進する2011

    • Author(s)
      藤田智史、Pytela Jaromir、加藤壮英、乾良充、神戸雅人、橋本隆
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      (仙台の予定が中止となり、3月11日付け年会講演要旨集のWeb公開をもって発表成立)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] PHS1はシロイヌナズナの間期表層微小管を制御する2010

    • Author(s)
      藤田智史、Pytela Jaromir、加藤壮英、乾良充、神戸雅人、橋本隆
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] シロイヌナズナ新規微小管調節因子PROPYZAMIDE HYPERSENSITIVE2(PHS2)の解析2010

    • Author(s)
      加藤壮英、橋本隆
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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