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Analysis of tubular elongation in the network of endoplasmic reticulum

Research Project

Project/Area Number 21570049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Hyogo

Principal Investigator

YOKOTA Etsuo  兵庫県立大学, 大学院・生命理学研究科, 助教 (80212299)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords小胞体 / レティキュロン / RHD3 / プロテインキナーゼ / タンパク質リン酸化 / ダイナソー / GTP / リン酸化 / シロイヌナズナ / キナーゼ / ミオシン / タバコ培養細胞BY-2 / GTPアーゼ / アトラスチン / 小胞体チューブ / ダイナミン
Research Abstract

In the cultured cell of Arabidopsis, MM2d, it was shown that reticulon-like proteins(AtRTN) and RHD3, a homologue of atlastin GTPase, are involved in the tubular formation of endoplasmic reticulum(ER), as is case of yeast and animal cells. The RHD3 was able to bind to GTP, and form the oligomer under the conditions in which ER tubules were organized. Furthermore, ER tubule formation was suggested to be regulated by the phosphorylation.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (11 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured cells, BY-22011

    • Author(s)
      E. Yokota, H. Ueda, K. Hashimoto, H. Orii, T. Shimada, I. Hara-Nishimura, T. Shimmen
    • Journal Title

      Plant Physiol

      Volume: 156 Issue: 1 Pages: 129-143

    • DOI

      10.1104/pp.111.175018

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea2011

    • Author(s)
      Hashimoto, K., Yokota, E., Shimmen, T., and Yoshida, M.
    • Journal Title

      Biotechnol. Lett.

      Volume: 33 Issue: 4 Pages: 769-775

    • DOI

      10.1007/s10529-010-0497-0

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of myosin ATPase inhibitor 2,3-butanedione 2-monoxime on protein secretion of basidiomycete Coprinopsis cinerea.2011

    • Author(s)
      K.Hashimoto
    • Journal Title

      Biotechnology Letters

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured cells, BY-2.2011

    • Author(s)
      E.Yokota
    • Journal Title

      Plant Physiol.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells2010

    • Author(s)
      Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawa, S., Dolja, V. V., and Hara-Nishimura, I.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107 Issue: 15 Pages: 6894-6899

    • DOI

      10.1073/pnas.0911482107

    • NAID

      120002511346

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells.2010

    • Author(s)
      H.Ueda
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 6894-6899

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells2010

    • Author(s)
      Ueda, Haruko
    • Journal Title

      Proceeding of the National Academy of Sciences, USA (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco2009

    • Author(s)
      Hamada, T., Igarashi, H., Taguchi, R., Fujiwara, M., Fukao, Y., Shimmen, T., Yokota, E., and Sonobe. S.
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Issue: 4 Pages: 801-811

    • DOI

      10.1093/pcp/pcp024

    • NAID

      10027339590

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells2009

    • Author(s)
      E. Yokota, S. Ueda, K. Tamura, H. Orii, S. Uchi, S. Sonobe, I. Hara-Nishimura, T. Shimmen
    • Journal Title

      J. Exp. Bot

      Volume: 60 Pages: 197-212

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis2009

    • Author(s)
      I. Karahara, J. Suda, H. Tahara, E. Yokota, T. Shimmen, K. Misaki, S. Yonemura, T. Murata, L. A. Staehelin, Y. Mineyuki
    • Journal Title

      Plant J

      Volume: 57 Pages: 819-831

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells2009

    • Author(s)
      Yokota, Etsuo
    • Journal Title

      Journal of Experimental Botany 60

      Pages: 197-212

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco2009

    • Author(s)
      Hamada, Takahiro
    • Journal Title

      Plant and Cell Physiology 50

      Pages: 801-811

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis2009

    • Author(s)
      Karahara, Ichirou
    • Journal Title

      The Plant Journal 57

      Pages: 819-831

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物細胞における小胞体ダイナミクス2012

    • Author(s)
      上田晴子
    • Organizer
      第14回植物オルガネラワークショップ
    • Place of Presentation
      京都大益川ホール(京都府)
    • Year and Date
      2012-03-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化2011

    • Author(s)
      上田晴子
    • Organizer
      日本生化学会第84回大会
    • Place of Presentation
      京都国際会館(京都府)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化2011

    • Author(s)
      上田晴子
    • Organizer
      日本生化学会第84回大会
    • Place of Presentation
      京都国際会館(京都府)(招待講演)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 接合菌類ヒゲカビの原形質流動を担うミオシンの同定2011

    • Author(s)
      横田悦雄
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス(東京都)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin organizations2011

    • Author(s)
      H. Ueda
    • Organizer
      International Botanical Congress
    • Place of Presentation
      メルボルンン(オーストラリア)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin organizations2011

    • Author(s)
      上田晴子
    • Organizer
      International Botanical Congress 2011
    • Place of Presentation
      メルボルン(オーストラリア)(招待講演)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物細胞における小胞体流動~定量解析と生理学的意義~2010

    • Author(s)
      上田晴子
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(愛知県)
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割2010

    • Author(s)
      上田晴子
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学(熊本県)
    • Year and Date
      2010-03-21
    • Related Report
      2011 Final Research Report
  • [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割2010

    • Author(s)
      上田晴子
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 小胞体チューブ状構造形成におけるアクチン系細胞骨格の役割2009

    • Author(s)
      横田悦雄
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学(山形県)
    • Year and Date
      2009-09-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 小胞体チューブ状構造形成におけるアクチン系細胞骨格の役割2009

    • Author(s)
      横田悦雄
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Book] Plant myosins. In The Plant Cytoskeletons.2011

    • Author(s)
      E.Yokota
    • Total Pages
      24
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Book] The Plant Cytoskeletons2010

    • Author(s)
      E. Yokota, T. Shimmen
    • Publisher
      Springer
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biomecha/index-jhtml

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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