Structural analysis of the transient state of an enzyme active site using NMR relaxation dispersion
| Project/Area Number |
21570170
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 核磁気共鳴 / NMR / 遷移状態 / induced-fit / 構造解析 / CPMG / 緩和分散 / 残余双極子相互作用 |
|---|
| Research Abstract |
An enzyme chitinase was aligned along the static magnetic field using Pf1-phage or acrylamide gel as an alignment medium. In this state residual dipolar coupling was observed, confirming that the system was effective for the analysis. To further apply the relaxation dispersion method to the alignment system, a complex system which was well known to be in a conformational exchange was used. Upon changing the stoichiometry in the complex, it was observed that the residual dipolar coupling also changed accordingly.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Binding Energetics of Ferredoxin-NADP^+ Reductase with Ferredoxin and Its Relation to Function2011
Author(s)
Lee, Y. H., Ikegami, T., Standley, D. M., Sakurai, K., Hase, T., and Goto, Y.
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Journal Title
Chembiochem
Volume: 12
Pages: 2062-2070
Related Report
Peer Reviewed
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[Journal Article] Immunogenicity of whole-parasite vaccines against Plasmodium falciparum involves malarial hemozoin and host TLR9.2010
Author(s)
Coban, C., Yagi, M., Ikegami, T., Ishii, K.J., Akira, S., et al.
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Journal Title
Cell Host Microbe
Volume: 7
Pages: 50-61
Related Report
Peer Reviewed
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[Journal Article] Arabidopsis Tic62 and ferredoxin-NADP(H) oxidoreductase form light-regulated complexes that are integrated into the chloroplast redox poise2009
Author(s)
Benz, J.P., Stengel, A., Lintala, M., Lee, Y.H., Weber, A., Philippar, K., Gugel, I.L., Kaieda, S., Ikegami, T., Mulo, P., Soll.J., Bolter.B
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Journal Title
Plant Cell 21
Pages: 3965-3983
Related Report
Peer Reviewed
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[Journal Article] Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii2009
Author(s)
Kuwahara, Y., Unzai, Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., Hiroaki, H.
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Journal Title
Biophysical Journal 97
Pages: 2034-2043
Related Report
Peer Reviewed
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