| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Our research group previously published data demonstrate that of siRNA targeting conserved regions of the untranscribed HIV-1 promoter region, a single dose of either Prom A siRNA induced prolonged and profound suppression of HIV re-production. Definitive evidence of transcriptional silencing (TGS) was provided to this repression of HIV reproduction by several assays. There is accumulating evidence the Argonaute family of siRNA binding proteins, play a pivotal role in siRNA induced TGS. In TGS Ago1 protein accumulates at the site of initiation of silencing. To explore the role of Ago1 in siRNA induced TGS of HIV-1, in vitro binding assay was established for detecting AGO1-siRNA complex can be bind to target DNA. This assay system clearly demonstrated that these complex canb ebind directly to taget DNA sequence. AGO1 protein was co-localized with siRNA when the cells infected HIV-1 by immune-staining assay. We also demonstrated that these TGS effects are dependent upon close matching between the siRNA and the target sequence.
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