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Characterization and formation of coiled-coil structure in the silk produced by hornet larvae

Research Project

Project/Area Number 21580072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

KAMEDA Tsunenori  独立行政法人農業生物資源研究所, 新機能素材研究開発ユニット, 主任研究員 (70334042)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシルク / スズメバチ / タンパク質 / 高次構造 / コイルドコイル / ゲル / フィルム / 昆虫素材
Research Abstract

Hornet silks, fibrous proteins present in the cocoon produced by the larvae of vespa species, are composed of four major proteins. Complete amino acid sequences of the four major proteins(Vssilk 1-4) in the hornet silk produced by the yellow hornet(Vespa simillima, Vespinae, Vespidae) have been determined. The amino acid sequences in Vssilk 1-4 are highly divergent, but the four proteins have some common properties. The most attractive feature of these proteins is that they have anα-helix region, which includes coiled-coilα-helices, and aβ-sheet region. The aforementioned coiled-coil andβ-sheet structures are responsible for the intermolecular binding between the Vssilk 1-4 proteins that make up the composite structure in hornet silk. This coiled-coil structure is restored when hornet silk gel films are formed by pressing and drying hornet silk hydrogel.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (43 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 5 results) Presentation (29 results) Book (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Identification of Hornet Silk Genewith a Characteristic Repetitive Sequence in Vespa simillima xanthoptera2012

    • Author(s)
      Kameda, T., Kojima, K., Zhang, Q. and Sezutsu, H.
    • Journal Title

      Comparative Biochemistry and Physiology

      Volume: B, 161 Pages: 17-24

    • Related Report
      2011 Final Research Report
  • [Journal Article] Identification of Hornet Silk Genewith a Characteristic Repetitive Sequence in Vespa simillima xanthoptera2012

    • Author(s)
      Kameda, T., Kojima, K., Zhang, Q., Sezutsu, H.
    • Journal Title

      Comparative Biochemistry and Physiology, B

      Volume: 161 Pages: 17-24

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of supercooling and organic s olvent on the formation of a silk sponge with porous 3-D structure, and its dyna mical and structural characterization using solid-state NMR2011

    • Author(s)
      Kameda, T., Hashimoto, T. and Tamada, Y.
    • Journal Title

      Journal of Materials S cience

      Volume: 46 Pages: 7923-7930

    • Related Report
      2011 Final Research Report
  • [Journal Article] コイルドコイル形成タンパク質からなるスズメバチのシルク2011

    • Author(s)
      亀田恒徳、小島桂、瀬筒秀樹、張薔、寺本英敏、玉田靖
    • Journal Title

      高分子論文集

      Volume: 67 Pages: 641-653

    • NAID

      10027624133

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] Effects of supercooling and organic solvent on the formation of a silk sponge with porous 3-D structure, and its dynamical and structural characterization using solid-state NMR2011

    • Author(s)
      Kameda, T., Hashimoto, T., Tamada, Y.
    • Journal Title

      Journal of Materials Science

      Volume: 46 Pages: 7923-7930

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drawing-Induced Changes in Morphology and Mechanical Properties of Hornet Silk Gel Films2010

    • Author(s)
      Tsunenori Kameda, Katsura Kojima, Eiji Togawa, Hideki Sezutsu, Qiang Zhang, Hidetoshi Teramoto and Yasushi Tamada
    • Journal Title

      Biomacromolecules

      Volume: 15 Pages: 1002-1008

    • Related Report
      2011 Final Research Report
  • [Journal Article] Drawing-Induced Changes in Morphology and Mechanical Properties of Hornet Silk Gel Films2010

    • Author(s)
      Tsunenori Kameda, Katsura Kojima, Eiji Togawa, Hideki Sezutsu, Qiang Zhang, Hidetoshi Teramoto, Yasushi Tamada
    • Journal Title

      Biomacromolecules 15

      Pages: 1002-1008

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production a nd Characterizations of Tubes from Hornet(Vespa) Silk

    • Author(s)
      Kameda, T. and Aratani, E
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: (印刷中)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Quantifying the fraction of alanine residues in anτ-helical conformation in hornet silk using solid-state NMR

    • Author(s)
      Kameda, T.
    • Journal Title

      Polymer Journal

      Volume: (印刷中)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Production and Characterizations of Tubes from Hornet (Vespa) Silk

    • Author(s)
      Kameda, T., Aratani, E.
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: (印刷中)

    • NAID

      10030798704

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantifying the fraction of alanine residues in an α-helical conformation in hornet silk using solid-state NMR

    • Author(s)
      Kameda, T.
    • Journal Title

      Polymer Journal

      Volume: (印刷中)

    • NAID

      10030931753

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] シルクで作るタンパク質素材の新展開2011

    • Author(s)
      亀田恒徳
    • Organizer
      アグリビジネス創出フェア
    • Place of Presentation
      幕張
    • Year and Date
      2011-12-02
    • Related Report
      2011 Final Research Report
  • [Presentation] シルクで作るタンパク質素材の新展開2011

    • Author(s)
      亀田恒徳
    • Organizer
      アグリビジネス創出フェア
    • Place of Presentation
      幕張(招待講演)
    • Year and Date
      2011-12-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] シルク素材の最前線2011

    • Author(s)
      亀田恒徳
    • Organizer
      バイオジャパン2011
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-10-05
    • Related Report
      2011 Final Research Report
  • [Presentation] シルク素材の最前線2011

    • Author(s)
      亀田恒徳
    • Organizer
      バイオジャパン2011
    • Place of Presentation
      パシフィコ横浜(招待講演)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] シルク蛋白質ゲルで作る成形体の構造と物性2011

    • Author(s)
      亀田恒徳
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2011-09-29
    • Related Report
      2011 Final Research Report
  • [Presentation] シルク蛋白質ゲルで作る成形体の構造と物性2011

    • Author(s)
      亀田恒徳
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学津島キャンパス(招待講演)
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新しいバイオマテリアルとしてのホーネットシルク2011

    • Author(s)
      亀田恒徳
    • Organizer
      第5回「フィプロインセリシンの利用」研究会
    • Place of Presentation
      秋葉原コンベンションホール
    • Year and Date
      2011-02-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hydrogen-bonding network and rotational motion of Ser side-chain studied by solid-state N MR2011

    • Author(s)
      Tsunenori Kameda, Hidetoshi Teramoto, Daisuke Hashizume, Hiroyuki Koshino, Katsuyuki Nishimur
    • Organizer
      第50回NMR討論会
    • Place of Presentation
      横浜大さんばしホール
    • Related Report
      2011 Final Research Report
  • [Presentation] ハチが作るシルクの構造的特徴2011

    • Author(s)
      亀田恒徳・小島桂・張薔・瀬筒秀樹・玉田靖
    • Organizer
      平成23年度蚕糸・昆虫機能利用学術講演会
    • Related Report
      2011 Final Research Report
  • [Presentation] Hydrogen-bonding network and rotational motion of Ser side-chain studied by solid-state NMR2011

    • Author(s)
      Tsunenori Kameda, Hidetoshi Teramoto, Daisuke Hashizume, Hiroyuki Koshino, Katsuyuki Nishimur
    • Organizer
      第50回NMR討論会
    • Place of Presentation
      横浜大さんばしホール
    • Related Report
      2011 Annual Research Report
  • [Presentation] HORNET(VESPA) SILK2010

    • Author(s)
      Kameda, T.
    • Organizer
      6 th INTERNATIONAL CONFERENCE O N WILD SILKMOTHS 2010
    • Place of Presentation
      Tokyo University of Agriculture
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] ホーネットシルクの構造と素材化2010

    • Author(s)
      亀田恒徳
    • Organizer
      「群馬大学ファイブロバイオプロセス研究会」第5回研究会
    • Place of Presentation
      桐生地域地場産業振興センター
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] HORNET (VESPA) SILK2010

    • Author(s)
      Kameda, T.
    • Organizer
      6th INTERNATIONAL CONFERENCE ON WILD SILKMOTHS 2010
    • Place of Presentation
      Tokyo University of Agriculture
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蜂シルクの特徴を活かした素材加工2010

    • Author(s)
      亀田恒徳・張薔・小島桂・寺本英敏・玉田靖
    • Organizer
      第57回日本シルク学会研究発表会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-06-18
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 新たな医療用フィルム素材としてのホーネットシルクの利用2010

    • Author(s)
      亀田恒徳,張薔,玉田靖
    • Organizer
      繊維学会年次大会
    • Place of Presentation
      東京(船堀)
    • Year and Date
      2010-06-16
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] シルクタンパク質によるチューブ状成形体の作製とキャラクタリゼーション2010

    • Author(s)
      亀田恒徳、寺本英敏、玉田靖
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Hornet(Vespa) silk : Structural studies by solid-state NMR2010

    • Author(s)
      Kameda, T.
    • Organizer
      Pacificchem2010
    • Place of Presentation
      米国(ハワイ)
    • Related Report
      2011 Final Research Report
  • [Presentation] シルクタンパク質によるチューブ状成形体の作製とキャラクタリゼーション2010

    • Author(s)
      亀田恒徳、寺本英敏、玉田靖
    • Organizer
      第59回 高分子討論会
    • Place of Presentation
      北海道大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hornet (Vespa) silk : Structural studies by solid-state NMR2010

    • Author(s)
      Kameda, T.
    • Organizer
      Pacificchem2010
    • Place of Presentation
      米国(ハワイ)
    • Related Report
      2010 Annual Research Report
  • [Presentation] スズメバチの繭の固体NMR構造解析からタンパク質材料の高強度化技術を考える2009

    • Author(s)
      亀田恒徳
    • Organizer
      理研シンポジウム「第10回分析・解析技術と化学の最先端」
    • Place of Presentation
      理化学研究所
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] スズメバチのシルクの延伸配向挙動に関する固体NMR解析2009

    • Author(s)
      亀田恒徳
    • Organizer
      第48回NMR討論会
    • Place of Presentation
      九州大学
    • Year and Date
      2009-11-11
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 固体NMRでタンパク質材料の高強度化の鍵を解く2009

    • Author(s)
      亀田恒徳
    • Organizer
      第46回固体NMR・材料フォーラム
    • Place of Presentation
      東京理科大学
    • Year and Date
      2009-10-13
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] スズメバチのシルクの階層構造制御とキャラクタリゼーション2009

    • Author(s)
      亀田恒徳,張薔,戸川英二,玉田靖
    • Organizer
      第58回高分子討論会
    • Place of Presentation
      熊本大学
    • Year and Date
      2009-09-17
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] ホーネットシルクフィルムの延伸過程における構造と物性の変化2009

    • Author(s)
      亀田恒徳、張薔、秦珠子、玉田靖
    • Organizer
      平成21年度繊維学会年次大会
    • Place of Presentation
      タワーホール船堀
    • Year and Date
      2009-06-10
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] スズメバチのシルクから作製したフィルムの延伸2009

    • Author(s)
      亀田恒徳,小島桂,張薔,寺本英敏,桑名芳彦,玉田靖
    • Organizer
      第58回高分子学会年次大会
    • Year and Date
      2009-05-27
    • Related Report
      2011 Final Research Report
  • [Presentation] スズメバチのシルクから作製したフィルムの延伸2009

    • Author(s)
      亀田恒徳, 小島桂, 張薔, 寺本英敏, 桑名芳彦, 玉田靖
    • Organizer
      第58回高分子学会年次大会
    • Place of Presentation
      新型インフルエンザ流行のため中止(予稿集公表)
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ハチが作る有用物質の固体NM R構造解析2009

    • Author(s)
      亀田恒徳
    • Organizer
      09-1 NMR研究会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2009-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] ハチが作る有用物質の固体NMR構造解析2009

    • Author(s)
      亀田恒徳
    • Organizer
      09-1 NMR研究会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2009-05-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] ハチが作るシルクの構造的特徴

    • Author(s)
      亀田恒徳・小島桂・張薔・瀬筒秀樹・玉田靖
    • Organizer
      平成23年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      (東日本大震災のため大会は中止)(要旨のみ公表)
    • Related Report
      2011 Annual Research Report
  • [Book] Nuclear Magnetic Resonance, Vol.41, "Applications of nuclear shielding"2012

    • Author(s)
      Kuroki, S., Kameda, T., Yasunag, H.
    • Total Pages
      62
    • Publisher
      Royal Society of Chemistry
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 筒状構造物及びその製造方法2011

    • Inventor(s)
      亀田恒徳,寺本英敏,玉田靖
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Filing Date
      2011-08-31
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 筒状構造物及びその製造方法2011

    • Inventor(s)
      亀田恒徳, 寺本英敏, 玉田靖
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Filing Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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