Characterization of φRSL1-encoded genes controlling the host virulence
| Project/Area Number |
21580095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMADA Takashi Hiroshima University, 大学院・先端物質科学研究科, 教授 (40230461)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIE Makoto 広島大学, 大学院・先端物質科学研究科, 准教授 (20274110)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 微生物機能 / ゲノム / ファージ / 植物病理 / マイクロアレイ / 応用微生物 / バクテリオファージ / DNAマイクロアレイ |
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| Research Abstract |
Based on DNA microarray analysis, 343 genes encoded by R.solanacearum jumbo phage φRSL1 were characterized to be expressed in the early, early-intermediate, intermediadte-late, and late stages. The φRSL1 genome consists of four gene-cluster regions where region I mainly contains early-intermediate genes, while most intermediate-late genes are located within regions II and IV. Bacterial cells infected with φRSL1 were steadily maintained at a low level of cell density over a long period. Under these conditions, 12 φRSL1 genes were shown to be highly expressed and regulate the host growth.
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Report
(3 results)
Research Products
(42 results)
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[Journal Article] Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages.2011
Author(s)
Fujiwara, A., Fujisawa, M., Hamasaki, R., Kawasaki, T., Fujie, M., Yamada T.
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Journal Title
Appl.Environ.Microbiol. In press.
Related Report
Peer Reviewed
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[Presentation] Comprehensive analysis of phage-tolerance mechanisms in Ralstonia sol anacearum2010
Author(s)
Fujiwara, A, Kotera S, Kawasaki, T, Fujie, M, Yamada, T
Organizer
Virus of Microbes
Place of Presentation
Institut Pasteur, Paris, France
Year and Date
2010-06-24
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