| Project/Area Number |
21580151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Okayama Prefectural University |
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Principal Investigator |
TSUJI Hideaki 岡山県立大学, 保健福祉学部, 教授 (20093875)
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| Co-Investigator(Kenkyū-buntansha) |
KIMOTO Masumi 岡山県立大学, 保健福祉学部, 教授 (40108866)
HIEMORI Miki 岡山県立大学, 保健福祉学部, 准教授 (80326412)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 大豆 / 小麦 / アレルゲン / 糖鎖 / 共通抗原 / 発現タンパク質 / Gly m Bd 28K / Tri a Bd 27K / 大豆アレルゲン / アレルゲン性 / 糖タンパク質 / 共通抗原性 / 糖ペプチド / アスパラギン結合型糖鎖 |
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| Research Abstract |
We investigated the allergenicity of soybean allergen, Gly m Bd 28K, and wheat allergen, Tri a Bd 27K. These allergens were shown to contain fucose and xylose in addition to mannose, N-acetyl glucosamine, indicating that the sugar moieties of these allergens may be asparagine-linmked sugar chain. Furthermore, these sugar moieties were shown to be bind IgE antibodies in sera of patients sensitive to soybean and wheat.
The cDNA encoding Gly m Bd 28K has been cloned. However, the cDNA has the part encoding the signal peptide of the precursor for Gly m Bd 28K, but the cDNA has not the perfect cDNA encoding full length of mRNA for the allergen. In the present study, three amino acid residues on the signal peputide and 20-bp on the 5'-untranlated region were elucidated by 5'-RACE kit. This finding provide useful information on the expression of the allergen in cultivated tabacco cells.
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