Characterization of unique properties in expression of ligninolytic enzymes by white-rot fungi

• Project/Area Number: 21580402
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Kyoto University
• Principal Investigator: HONDA Yoichi 京都大学, 大学院・農学研究科, 教授 (70252517)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 木材腐朽菌 / バイオマス / リグニン分解 / 遺伝子組換え / 担子菌 / プロモーター / 菌類 / 遺伝子 / 応用微生物 / 遺伝子発現 / タンパク質 / 蛋白質

Research Abstract

Lignocellulosic resources are expected as sustainable resources which can be converted to useful materials such as bio-ethanol or biodegradable plastics and not competing with utilization as foods. However, effective elimination of lignin is a bottle neck in the conversion process of lignocellulosics and white-rot fungi can solve this problem providing specific enzymes which are very difficult to produce by heterologous organisms. In this study, basic properties specific for expression of proteins have been analyzed in white-rot fungi, such as Pleurotus ostreatus and Ceriporiopsis subvermispora

Research Products

• [Journal Article] Enhanced green fluorescent protein expressioninPleurotusostreatusforin vivoanalysisoffungallaccasepromoters (2012)
  • Author(s): AntonellaAmore,YoichiHondaandVincenza Faraco
  • Journal Title: Appl.Biochem.Biotechnol Volume: 15, Aug. Issue: 4 Pages: 761-769
  • DOI: 10.1007/s12010-012-9816-3
  • Peer Reviewed
• [Journal Article] Copper induction of enhanced green fluorescent protein expression in Pleurotus ostreatus driven by laccase poxa1bpromoterAntonellaAmore (2012)
  • Author(s): Yoichi HondaandVincenzaFaraco
  • Journal Title: FEMSMicrobiol Lett Volume: 337 Pages: 155-163
  • Peer Reviewed
• [Journal Article] Enhanced green fluorescent protein expression in Pleurotus ostreatus for in vivo analysis of fungal laccase promoters. (2012)
  • Author(s): Amore A., Y. Honda and V. Faraco
  • Journal Title: FEMS Microbiol. Lett. Volume: 377 Issue: 2 Pages: 155-163
  • DOI: 10.1111/1574-6968.12023
  • Peer Reviewed
• [Journal Article] Epoxy ceriporic acid produced by selective lignin-degrading fungus Ceriporiopsis subvermispora (2011)
  • Author(s): Nishimura, H., Y.Setogawa, T.Watanabe, Y.Honda, T.Watanabe
  • Journal Title: Chem.Phys.Lipids Volume: (in press) Issue: 8 Pages: 707-712
  • DOI: 10.1016/j.chemphyslip.2011.07.005
  • Peer Reviewed
• [Journal Article] Diverse rare lipid-related metabolites including <omega>-7 and <omega>-9 alkenylitaconic acids (ceriporic acids) secreted by a selective white rot fungus, Ceriporiopsis subvermispora (2011)
  • Author(s): Nishimura, H., K.Murayama, T.Watanabe, Y.Honda, T.Watanabe
  • Journal Title: Chem.Phys.Lipids Volume: (in press) Issue: 1 Pages: 97-104
  • DOI: 10.1016/j.chemphyslip.2011.10.007
  • Peer Reviewed
• [Journal Article] Characterization of a Δ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus (2010)
  • Author(s): Watanabe, T., S.Tsuda, H.Nishimura, Y.Honda, T.Watanabe
  • Journal Title: Appl.Microbiol.Biotechnol. Volume: 87 Pages: 215-224
  • Peer Reviewed
• [Journal Article] Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium (2010)
  • Author(s): Sakamoto, T., H.Kitaura, M.Minami, Y.Honda, T, Watanabe, A, Ueda, K, Suzuki, T.Irie
  • Journal Title: Curr Genet Volume: 56 Pages: 401-410
  • Peer Reviewed
• [Journal Article] Absolute configuration of ceriporic acids, the iron redox-silencing metabolites produced by a selective (2009)
  • Author(s): Nishimura, H., K.Murayama, T.Watanabe, Y.Honda, T.
  • Journal Title: Chem. Phys. Lipids 159 Pages: 77-80
  • Peer Reviewed
• [Journal Article] Degradation of sulfide linkages between isoprenes by lipid peroxidation catalyzed by manganese peroxidase (2009)
  • Author(s): Sato, S., Y.Ohashi, M.Kojima, T.Watanabe, Y.Honda, T.
  • Journal Title: Chemosphere 77 Pages: 798-804
  • Peer Reviewed
• [Presentation] A new system for in vivo analyses of Pleurotus ostreatus promoters of laccases for food and beverages industries (2012)
  • Author(s): Antonella Amore, Yoichi Honda and Vincenza Faraco
  • Organizer: International Conference on Industria Biotechnology (ICIB 2012)
  • Place of Presentation: Patiala (India)
• [Presentation] A new system for in vivo analyses of Pleurotus ostreatus promoters of laccases for food and beverages industries (2012)
  • Author(s): Amore A., Y. Honda and V. Faraco
  • Organizer: International Conference on Industrial Biotechnology
  • Place of Presentation: Patiala, India
• [Presentation] Mutational analysis of GPD promoter sequence using a transient gene expression-A new promoter assay system for basidomycetous fungi (2011)
  • Author(s): HondaY., Tanigawa E. Watanabe T. and T. Watanabe
  • Organizer: The 7 International Conference of the WorldSociet fo Mushroo Biolog and Mushroom Products
  • Place of Presentation: Arcachon, France
• [Presentation] Effects of cAMP in ligninolytic enzymes production (2011)
  • Author(s): Yao Y., T.Sakamoto, Y.Honda, T.Watanabe, W.Hashigaya, K.Suzuki, T.Irie
  • Organizer: International Union of Microbiological Societies 2011 Congress, XIII International Congress of Mycology
  • Place of Presentation: Sapporo Convention Center
• [Presentation] Screening of calmodulin-binding proteins expressed during ligninolytic enzyme production (2011)
  • Author(s): Sakamoto, T, Y.Honda, I.Kameshita, T.Watanabe, K.Suzuki, T.Irie
  • Organizer: International Union of Microbiological Societies 2011 Congress, XIII International Congress of Mycology
  • Place of Presentation: Sapporo Convention Center
• [Presentation] Mutational analysis of GPD promoter sequence using a transient gene expression -A new promoter assay system for basidomycetous fungi- (2011)
  • Author(s): Honda Y., Tanigawa E., Watanabe T., T.Watanabe
  • Organizer: The 7 International Conference of the World Society for Mushroom Biology and Mushroom Products
  • Place of Presentation: Arcachon, France
• [Presentation] Transient gene expression and promoter analysis in basidiomycete (2010)
  • Author(s): Y.Honda, E.Tanigawa, T.Watanabe, T.Watanabe
  • Organizer: The 6th Meeting of East Asia for Mushroom Science 2010
  • Place of Presentation: 慶州(韓国)
  • Year and Date: 2010-11-14
• [Presentation] Genetic transformation and promter assay systems in selective lignin degrader, Ceriporiopsis subvermispora (2010)
  • Author(s): Y.Honda, E.Tanigawa, H.Kawabe, J.Watari, T.Watanabe T.
  • Organizer: Lignobiotech One
  • Place of Presentation: Reims (France)
  • Year and Date: 2010-03-30
• [Presentation] 選択的リグニン分解菌Ceriporiopsis subvermisporaの安定形質転換系とプロモーターアッセイ系の開発 (2010)
  • Author(s): 谷川瑛士、川辺陽文、渡利純子、渡邊崇人、本田与一、渡辺隆司
  • Organizer: 日本木材学会第60回大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-03-18
• [Presentation] Honda Y., E. Tanigawa, Takahito Watanabe and Takashi Watanabe (2010)
  • Author(s): Honda Y., E. Tanigawa, Takahito Watanabe and Takashi Watanabe
  • Organizer: The 6th meeting of East Asia for Mushroom Science 2010
  • Place of Presentation: Gyeongju,Korea
• [Presentation] gpd遺伝子プロモーターの解析:GCN4結合配列の機能について (2010)
  • Author(s): 本田与一, 谷川瑛二, 渡邊崇人, 渡辺隆司
  • Organizer: 日本きのこ学会第14回大会
  • Place of Presentation: 東京
• [Presentation] 担子菌における新規プロモーターアッセイ系の開発 (2010)
  • Author(s): 本田与一,谷川瑛二, 渡邊崇人, 渡辺隆司
  • Organizer: 日本菌学会第54回大会
  • Place of Presentation: 東京
• [Presentation] Genetic transformation and promoter assay systems in selective lignin degrader, Ceriporiopsis subvermispora, Lignobiotech one (2010)
  • Author(s): Honda, Y., E Tanigawa, H.Kawabe, J. Watari, Takahito Watanabe, Takashi Watanabe
  • Organizer: 1st Symposium on Biotechnolgy Applied to Lignocellulses
  • Place of Presentation: Reims, France
• [Presentation] 選択的リグニン分解菌Ceriporiopsis subvermisporaの安定形質転換系とプロモーターアッセイ系の開発 (2010)
  • Author(s): 谷川瑛二、川邊陽文、渡利純子、渡邊崇人、本田与一、渡辺隆司
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎
• [Presentation] 担子菌きのこにおける研究の現状と課題 (2009)
  • Author(s): 本田与一
  • Organizer: 日本遺伝学会第81回大会ワークショップ
  • Place of Presentation: 松本市
  • Year and Date: 2009-09-18
• [Presentation] Genetic transformation and its application in basidiomycete fungus, Pleurotus ostreatus (2009)
  • Author(s): Y.Honda
  • Organizer: 韓国微生物学連合50周年記念大会
  • Place of Presentation: 済州(韓国)
  • Year and Date: 2009-05-28