| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Functions of Peptide-N-Glycanase (PNGase) in endoplasmic reticulum-associated degradation (ERAD) are attracting recent attention. In order to analyze in this process, we developed a potent inhibitor having clickable tag that can allow for a variety of functional groups to be introduced such as detection and purification tags easily using a Cu (I)-catalyzed [3,2] cycloaddition reaction. Our probe inhibited PNGase selectively by micro M order and can easily lead to a variety of compounds. These compounds should help in the understanding of the biological functions of cytoplasmic PNGase.
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