Molecular and morphological analysis of RNA helicase that shows an unique expression in the nervous system by utilizing genetically modified animals

• Project/Area Number: 21590198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Wakayama Medical University
• Principal Investigator: TSURUO Yoshihiro 和歌山県立医科大学, 医学部, 教授 (90207449)
• Co-Investigator(Kenkyū-buntansha): UEYAMA Takashi 和歌山県立医科大学, 医学部, 准教授 (50264875) ; ITO Takao 和歌山県立医科大学, 医学部, 助教 (30315931)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: RNAヘリカーゼ / 神経系 / オリゴデンドロサイト / 遺伝子改変動物 / 分子形態学

Research Abstract

The monoclonal antibody(4F2) that specifically identifies oligodendrocytes was generated, and the molecule recognized by this antibody proved to be Ddx54, a member of RNA helicase. In the transgenic animals with forced expression of Ddx54, myelination appeared to be accelerated. The 4F2-positive cells were observed in the rat central nervous system from early embryonal to adult stages, and they were specifically expressed in oligodendrocytes using primary cultures of neural tissues. Ddx54 bound to 4 types of MBP isoforms, and especially the 21. 5 kDa isoform of MBP was transported into the nucleus, suggesting its involvement in the signal transduction during the process of myelination in oligodendrocytes.

Research Products

• [Journal Article] A new monoclonal antibody, 4F2, specific for the oligodendroglial cell lineage, recognizes ATP-dependent RNA helicase Ddx54 : possible association with myelin basic protein (2011)
  • Author(s): Ueki T, Tsuruo Y, Yamamoto Y, Yoshimura K, Takanaga H, Seiwa C, Motojima K, Asou H, Yamamoto M
  • Journal Title: J Neurosci Res 90 Volume: 90 Issue: 1 Pages: 48-59
  • DOI: 10.1002/jnr.22736
• [Journal Article] Cardiac and vascular gene profiles in an animal model of Takotsubo cardiomyopathy. (2011)
  • Author(s): Ueyama T, et al.
  • Journal Title: Heart and Vessels Volume: 26 Pages: 321-337
  • NAID: 10029835153
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral change. (2010)
  • Author(s): Yamamoto Y, et al.
  • Journal Title: J Immunol Volume: 229 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Up-regulation of hemeoxygenase 1 in an animal model of takotsubo cardiomyopathy (2009)
  • Author(s): Ueyama T, et al.
  • Journal Title: Circulation Journal 73 Pages: 1141-1146
  • NAID: 10026904800
  • Peer Reviewed
• [Journal Article] Polaprezinc(zinc L-carnosine)is a potent inducer of anti-oxidative stress enzyme, heme oxygenase(HO)1-a new mechanism of gastric mucosal protection (2009)
  • Author(s): Ueda K, et al.
  • Journal Title: J Pharma Sci 110 Pages: 285-294
  • Peer Reviewed
• [Presentation] オリゴデンドロサイトに特異的モノクローナル抗体(4F2)による新規分子マーカーの同定および機能解析 (2012)
  • Author(s): 山本悠太、鶴尾吉宏、志波歩美、伊藤隆雄、上山敬司、植木俊之、山本雅浩、阿相皓晃
  • Organizer: 日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス
  • Year and Date: 2012-03-26