The molecular mechanisms of migration of GnRH neurons and GnRH axon projection to the median eminence.

• Project/Area Number: 21590203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Juntendo University
• Principal Investigator: MURAKAMI Shizuko 順天堂大学, 医学部, 助教 (20255649)
• Co-Investigator(Kenkyū-buntansha): KOIKE Masato 順天堂大学, 医学部, 准教授 (80347210) ; SASAKI Mitsuho 順天堂大学, 医学部, 助教 (20432536)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: GnRHニューロン / 軸索ガイダンス分子 / セマフォリン3A / ニューロピリン1 / スリット / ニワトリ胚 / ニューロン移動 / 軸索伸長 / コラーゲンゲル3次元培養

Research Abstract

Migration assays in coculture explants of GnRH neurons in the embryonic chick dorsal septum region demonstrated that semaphorin 3A-neuropilin 1 signaling act as a chemorepulsive cue for the migration of GnRH neurons in the forebrain. In Slit1 ; Slit2 double mutant mice, GnRH neuron migration toward the caudal region was disturbed and GnRH axon projections to the median eminence were severely reduced. It is possible that the aberrant crossing fibers in the preoptic area led to a defect of GnRH neuron migration and a disruption of GnRH axonal pathfinding as a physical barrier.

Research Products

• [Journal Article] Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain (2010)
  • Author(s): Murakami S, Ohki-Hamazaki H, Watanabe K, Ikenaka K, Ono K
  • Journal Title: J Comp Neurol Volume: 518 Pages: 2019-2034
  • Peer Reviewed
• [Journal Article] Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain. (2010)
  • Author(s): Murakami, et al.
  • Journal Title: The Journal of Comparative Neurology Volume: 87 Pages: 2019-2034
  • Peer Reviewed
• [Journal Article] Neurotrophic effect of GnRH on neurite extension and neuronal migration of embryonic GnRH neurons in chick olfactory nerve bundle culture (2009)
  • Author(s): Kanaho Y-I, Enomoto M, Endo D, Maehiro S, Park MK, Murakami S
  • Journal Title: J Neurosci Res Volume: 87 Pages: 2237-2244
  • Peer Reviewed
• [Journal Article] Neurotrophic effect of GnRH on neurite extension and neuronal migration of embryonic GnRH neurons in chick olfactory nerve bundle culture (2009)
  • Author(s): Kanaho Y-I, et al.
  • Journal Title: J Neurosci Res 87 Pages: 2237-2244
  • Peer Reviewed
• [Journal Article] Cues guiding GnRH neuronal migration in the forebrain
  • Author(s): Murakami S
  • Journal Title: J Physiol Sci Volume: 61
• [Presentation] The fate of cells expressing somatostatin mRNA and co-migrating with GnRH neurons from the olfactory epithelium to the forebrain (2011)
  • Author(s): 村上志津子, 他2名
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-14
• [Presentation] Semaphorin 3A provides a chemorepulsive guidance cue to migrating GnRH neurons along the olfactory nerve (2010)
  • Author(s): 村上志津子、小野勝彦、内山安男
  • Organizer: Neuro 2010第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2010-09-04
• [Presentation] Semaphorin 3A provides a chemorepulsive guidance cue to migrating GnRH neurons along the olfactory nerve. (2010)
  • Author(s): 村上志津子, 他2名
  • Organizer: Neuro 2010 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-04
• [Presentation] GnRHニューロンの移動におけるセマフォリン3Aの反発活性 (2010)
  • Author(s): 村上志津子、小野勝彦、内山安男
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館(盛岡)
  • Year and Date: 2010-03-28
• [Presentation] GnRHニューロンの移動におけるセマフォリン3Aの反発活性 (2010)
  • Author(s): 村上志津子, 他2名
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 2010-03-28
• [Presentation] GnRHニューロンと共に鼻から脳へ移動するソマトスタチン細胞 (2009)
  • Author(s): 村上志津子、浜崎浩子、内山安男
  • Organizer: 第114回日本解剖学会総会,第34回日本比較内分泌学会・第31回日本比較生理生化学大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2009-10-23
• [Presentation] GnRHニューロンと共に鼻から脳へ移動するソマトスタチン細胞 (2009)
  • Author(s): 村上志津子
  • Organizer: 第34回日本比較内分泌学会・第31回日本比較生理生化学大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-10-23
• [Presentation] Co-migration of somatostatin mRNA-expressing cells with GnRH neurons from the olfactory placode to the forebrain (2009)
  • Author(s): 村上志津子、浜崎浩子、内山安男
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2009-09-16
• [Presentation] Co-migration of somatostatin mRNA-expressing cells with GnRH neurons from the olfactory placode to the forebrain (2009)
  • Author(s): 村上志津子
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-16
• [Presentation] GnRHニューロンと共に鼻から脳へ移動するソマトスタチン細胞 (2009)
  • Author(s): 村上志津子、浜崎浩子、内山安男
  • Organizer: 第114回日本解剖学会総会
  • Place of Presentation: 岡山理科大学(岡山)
  • Year and Date: 2009-03-30