Research of the role of BNP and NOTCH signals for cardiac atrioventricular valve formation

• Project/Area Number: 21590205
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: INAI Kei 東京女子医科大学, 医学部, 講師 (80318063)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 発生学・形態形成学 / 心臓発生 / 房室弁 / NOTCHシグナル / BMP-2 / Notch

Research Abstract

Formation of the endocardial cushion mesenchyme in the atrioventricular(AV) canal is the essential morphogenetic step in cardiac valvuloseptal morphogenesis. Numerous studies demonstrated that BMP-2 signaling promotes mesencymal cells differentiation into pre-valvular fibroblasts.
In this study, we showed that BMP-2 induces expression of Notch1 and Notch pathway intermediates in the endocardial cushion mesenchymal cells(ECMC) aggregate culture. We also demonstrated that BMP-2 induces the valvulogenic phenotype including hyarulonan expression and cellular migration and inhibits expression of alternative mesodermal lineage markers in chick ECMC cultures.

Research Products

• [Journal Article] (2009)
  • Author(s): Florian Lang editor
  • Journal Title: Encyclopedia of molecular mechanisms of disease(Springer)
• [Presentation] ヒアルロン酸はPI3Kシグナルを介して心房室弁形成における細胞遊走を促進する (2010)
  • Author(s): 稲井慶
  • Organizer: 第9回心臓血管発生研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-10
• [Presentation] ヒアルロン酸はPI3Kシグナルを介して心房室弁形成における細胞遊走を促進する (2010)
  • Author(s): 稲井慶
  • Organizer: 第9回心臓血管発生研究会
  • Place of Presentation: シェラトングランデトーキョーベイホテル
  • Year and Date: 2010-07-10
• [Book] Florian Lang editor : Encyclopedia of molecular mechanisms of disease (2009)
  • Author(s): Kei Inai
  • Publisher: Springer