| Project/Area Number |
21590209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Gunma University |
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Principal Investigator |
AOKI Takeo 群馬大学, 大学院・医学系研究科, 講師 (70150919)
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Haruo 帝京大学, 医学部, 教授 (80189464)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUURA Tsutomu 群馬大学, 大学院・工学系研究科, 准教授 (80181692)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 膜微小ドメイン / アクアポリン-2 / リサイクリングシステム / MDCK細胞 / カベオラ / シンタキシン-6 / 初期エンドソーム / 糖脂質合成阻害剤 / アクアポリン2 / シンタキシン6 / カベオリン1 / コレステロール / RNAi / エンドサイトーシス |
|---|
| Research Abstract |
In MDCK cells stably expressing human AQP2 (MDCK-hAQP2), AQP2 is trafficked to the apical special membrane by forskolin and then internalized to the intracellular compartment by some mechanisms. The results of experiments in caveolin-1 knockdown cells by RNA interference or ceramide glucosyltransferase inhibitor treated cells, sialidase treated cells suggest that AQP2 expression and its retention in the apical membrane, its destination is closely related with the completeness of membrane microdomain components.
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