Studies on the mechanism of epithelial ion transport mediated by Chemical receptors expressed in the colonic mucosa

• Project/Area Number: 21590235
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Shizuoka
• Principal Investigator: KUWAHARA Atsukazu 静岡県立大学, 大学院・環境科学研究所, 教授 (60142890)
• Co-Investigator(Kenkyū-buntansha): KARAKI Shinichiro 静岡県立大学, 環境科学研究所, 助教 (00363903)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 消化管 / 化学受容細胞 / L型腸内分泌細胞 / プロスタグランジン / 短鎖脂肪酸受容体 / 苦味受容体 / 匂い受容体 / TRPA1チャネル / 食物繊維

Research Abstract

Gut lumen is continually exposed to many agents, including noxious compounds. Chemical receptors that detect the luminal environment are thought to play an important role as sensors and as modulators of epithelial cell functions. In the present research, function of chemical sensors in the rat and human large intestine were investigated.

Research Products

• [Journal Article] Activation of TRPA1 by luminal stimuli induces EP4-mediated anion secretion in human and rat colon (2012)
  • Author(s): Kaji I, Yasuoka Y, Karaki SI, Kuwahara A
  • Journal Title: Am.J.Physiol. Volume: 302(7)
  • Peer Reviewed
• [Journal Article] Chemosense for luminal environment in the large intestine (2011)
  • Author(s): Kaji I, Karaki SI and Kuwahara A
  • Journal Title: Yakugaku Zasshi Volume: 131 Pages: 1691-1698
  • NAID: 130001871765
  • Peer Reviewed
• [Journal Article] The G-protein on cholesterol-rich membrane microdomains mediates mucosal sensing of short-chain fatty acid and secretory response in rat colon (2011)
  • Author(s): Yajima T, Inoue R, Yajima M, Tsuruta T, Karaki SI, Hiroa T and Kuwahara A
  • Journal Title: Acta Physiol.(Oxf.) Volume: 203 Pages: 381-389
  • Peer Reviewed
• [Journal Article] Density distribution of free fatty acid receptors(FFA2)-expressing and GLP-1-producing enteroendocrine L cells in human and rat lower intestine, and increased cell numbers after ingestion of fructo-oligosaccharide (2011)
  • Author(s): Kaji I, Karaki SI, Tanaka R and Kuwahara A
  • Journal Title: J. Mol. Histol Volume: 42 Pages: 27-38
  • Peer Reviewed
• [Journal Article] Effects of luminal thymol on epithelial transport in human and rat colon (2011)
  • Author(s): Kaji I, Karaki SI and Kuwahara A
  • Journal Title: Am. J. Physiol Volume: 300
  • Peer Reviewed
• [Journal Article] Propionate-induced epithelial K+and Cl-/HCO3-secretion and free fatty acid receptor 2(FFA2, GPR43) expression in the guinea pig distal colon (2011)
  • Author(s): Karaki SI and Kuwahara A
  • Journal Title: Pflugers Arch Volume: 461 Pages: 461-152
  • Peer Reviewed
• [Journal Article] Chemosense for luminal environment in the large intestine (2011)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Journal Title: Yakugaku Zasshi Volume: 131(12) Pages: 1691-1698
  • NAID: 130001871765
  • Peer Reviewed
• [Journal Article] Effects of luminal thymol on epithelial transport in human and rat colon (2011)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Journal Title: Am.J.Physiol. Volume: 300(6)
  • Peer Reviewed
• [Journal Article] The G-protein on cholesterol-rich membrane microdomains mediates mucosal sensing of short-chain fatty acid and secretory response in rat colon (2011)
  • Author(s): Yajima T, Inoue R, Yajima M, Tsuruta T, Karaki, SI, Hira T, Kuwahara A
  • Journal Title: Acta Physiol. Volume: 203 Issue: 3 Pages: 381-389
  • DOI: 10.1111/j.1748-1716.2011.02331.x
  • Peer Reviewed
• [Journal Article] Density distribution of free fatty acid receptor 2 (FFA2)-expressing and GLP-1-producing enteroendocrine L cells in human and rat lower intestine, and increased cell numbers after ingestion of fructo-oligosaccharide (2011)
  • Author(s): Kaji I, Karaki SI, Tanaka R, Kuwahara A.
  • Journal Title: J.Molecular Histology Volume: 42 Pages: 27-38
  • Peer Reviewed
• [Journal Article] Propionate-induced epithelial K^+ and Cl^-/HCO_3^- secretion and free fatty acid receptor 2 (FFA2, GPR43)expression in the guinea pig distal colon (2011)
  • Author(s): Karaki SI, Kuwahara A
  • Journal Title: Pflugers Arch (European J.Physiol.) Volume: 461 Pages: 142-152
  • Peer Reviewed
• [Journal Article] Role of short-chain fatty acids and their receptors in colonic motility (2010)
  • Author(s): Karaki SI and Kuwahara A
  • Journal Title: Bioscience Microflora Volume: 29 Pages: 31-40
  • NAID: 10026975176
  • Peer Reviewed
• [Journal Article] Free fatty acid receptors and their physiological roles in the colon (2010)
  • Author(s): Karaki SI and Kuwahara A
  • Journal Title: Medycyna Wet Volume: 66 Pages: 147-155
  • Peer Reviewed
• [Journal Article] 大腸内の短鎖脂肪酸とその受容体による大腸運動の制御-腸内細菌と生体との橋渡しによる生体ホメオスタシスの制御 (2010)
  • Author(s): 唐木晋一郎、桑原厚和
  • Journal Title: 化学と生物 Volume: 48 Pages: 230-232
  • NAID: 10026438009
• [Journal Article] Role of short-chain fatty acids and their receptors in colonic motility (Review) (2010)
  • Author(s): Karaki SI, Kuwahara A
  • Journal Title: Bioscience Microflora Volume: 29 Pages: 31-40
  • Peer Reviewed
• [Journal Article] Free fatty acid receptors and their physiological roles in the colon (2010)
  • Author(s): Karaki SI, Kuwahara A.
  • Journal Title: Medycyna Wet 66 Pages: 147-155
  • Peer Reviewed
• [Journal Article] Secretory effects of a luminal bitter tastant and expressions of bitter taste receptors, T2Rs, in the human and rat large intestine (2009)
  • Author(s): Kaji I, Karaki SI, Fukami Y, Terasaki M and Kuwahara A
  • Journal Title: Am. J. Physiol Volume: 296
  • Peer Reviewed
• [Journal Article] Expression of short-chain fatty acid receptor GPR41 in the human colon (2009)
  • Author(s): Tazoe H, Otomo Y, Karaki SI, Kato I, Fukami Y, Terasaki M and Kuwahara A
  • Journal Title: Biomedical Res Volume: 30 Pages: 149-156
  • NAID: 130005449544
  • Peer Reviewed
• [Journal Article] Secretory effects of a luminal bitter tastant and expressions of bitter taste receptors, T2Rs, in the human and rat large intestine (2009)
  • Author(s): Kaji I, Karaki S, Fukami Y, Terasaki M, Kuwahara A.
  • Journal Title: Am.J.Physiol. 296
  • Peer Reviewed
• [Journal Article] Expression of short-chain fatty acid receptor GPR41 in the human colon (2009)
  • Author(s): Tazoe H, Otomo Y, Karaki SI, Kato I, Fukami Y, Terasaki M, Kuwahara A.
  • Journal Title: Biomed.Res. 30 Pages: 149-156
  • NAID: 130005449544
  • Peer Reviewed
• [Journal Article] Activation of TRPA1 by luminal stimuli induces EP4-mediated anion secretion in human and rat colon
  • Author(s): Kaji I, Yasuoka Y, Karaki SI and Kuwahara A
  • Journal Title: Am. J. Physiol Volume: (in press)
  • Peer Reviewed
• [Presentation] ラット結腸・縦走筋運動におけるEP受容体分布の詳細について (2011)
  • Author(s): 飯塚祐美子, 唐木晋一郎, 加治いずみ, 桑原厚和
  • Organizer: 第17回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学・神田キャンパス
  • Year and Date: 2011-12-17
• [Presentation] Mode of action of PGE_2 on the motility of longitudinal muscle in rat colon (2011)
  • Author(s): Iizuka Y, Karaki SI, Kaji I, Kuwahara A
  • Organizer: 7th Congress of the Federations of Asian and Oceanian Physiological Societies(FAOPS)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-11-14
• [Presentation] Secretory response to TRPA1 activation in rat and human colon (2011)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Organizer: 7th Congress of the Federations of Asian and Oceanian Physiological Societies(FAOPS)
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-11-14
• [Presentation] ヒト及びラット大腸粘膜の陰イオン分泌におけるTRPA1チャネルの関与 (2011)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 2011年度生理学研究所研究会『上皮細胞の恒常性維持機構におけるイオン・物質輸送の新しい分子生理』(自然科学研究機構自然科学研究機構基礎生物学研究所)
• [Presentation] ヒト及びラット大腸のイオン輸送作用における匂い物質受容の役割 (2010)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 第16回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学,東京都
  • Year and Date: 2010-12-04
• [Presentation] ラット中位結腸縦走筋におけるPGE2の運動促進作用に関与するプロスタグランジン受容体の同定 (2010)
  • Author(s): 飯塚祐美子, 唐木晋一郎, 田中亮, 加治いずみ, 桑原厚和
  • Organizer: 第16回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学,東京都
  • Year and Date: 2010-12-04
• [Presentation] 下部消化管における化学受容について (2010)
  • Author(s): 唐木晋一郎
  • Organizer: 第9回Hindgut Club Japanサテライトミーティング
  • Place of Presentation: 徳島市
  • Year and Date: 2010-05-22
• [Presentation] 消化管でのカリウムイオン輸送について (2010)
  • Author(s): 桑原厚和, 加治いずみ, 唐木晋一郎
  • Organizer: 第2回消化管セミナー
  • Place of Presentation: 北海道大学,札幌市
  • Year and Date: 2010-03-19
• [Presentation] 大腸機能に対する化学センサー受容体FFA2およびFFA3の役割 (2010)
  • Author(s): 桑原厚和, 唐木晋一郎, 加治いずみ
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市
• [Presentation] ラット結腸輪走筋の自発運動に対するプロスタグランジンE_2の作用 (2010)
  • Author(s): 唐木晋一郎, 田中亮, 加治いずみ, 桑原厚和
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市
• [Presentation] ヒト及びラット大腸における匂い物質の受容と分泌作用 (2010)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 生理研研究会『極性細胞の病態生理解明に向けた多角的アプローチ』
  • Place of Presentation: 自然科学研究機構生理学研究所,岡崎市
• [Presentation] ヒト及びラット大腸における匂い物質の感受と分泌作用 (2010)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市
• [Presentation] Fructo-origosaccharide(FOS) supplementation increases enteroendocrine L cells containing GLP-1 and SCFA receptor GPR43(FFA2) in the large intestine (2010)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Organizer: 2010 Digestive Disease Week
  • Place of Presentation: New Orleans, USA
• [Presentation] 下部消化管における化学受容について (2009)
  • Author(s): 唐木晋一郎
  • Organizer: 第15回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学
  • Year and Date: 2009-12-05
• [Presentation] プロスタグランジンE2受容体と大腸の自発運動との関係について (2009)
  • Author(s): 田中亮, 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 第15回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学
  • Year and Date: 2009-12-05
• [Presentation] ヒト及びラット下部消化管における苦味物質の受容と分泌作用 (2009)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 第15回Hindgut Club Japanシンポジウム
  • Place of Presentation: 専修大学
  • Year and Date: 2009-12-05
• [Presentation] ヒトおよびラット下部消化管における苦味受容体の生理機能に関する研究 (2009)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 第1回消化管セミナー「栄養素の認識と消化管機能:受容体とトランスポーターの役割」
  • Place of Presentation: 北海道大学創成科学共同研究棟
  • Year and Date: 2009-03-18
• [Presentation] 大腸運動と短鎖脂肪酸 (2009)
  • Author(s): 田副秀章, 唐木晋一郎, 桑原厚和
  • Organizer: 第1回消化管セミナー「栄養素の認識と消化管機能:受容体とトランスポーターの役割」
  • Place of Presentation: 北海道大学創成科学共同研究棟
  • Year and Date: 2009-03-18
• [Presentation] 消化管短鎖脂肪酸受容体の発現様式とその生理作用 (2009)
  • Author(s): 桑原厚和
  • Organizer: 第13回腸内細菌学会
  • Place of Presentation: 北里大学薬学部
• [Presentation] Distributions of short-chain fatty acid receptors GPR41 and GPR43 in the human colon (2009)
  • Author(s): Tazoe H, Otomo Y, Kaji I, Tanaka R, Karaki SI, Kuwahara A
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] ヒト及びラット下部消化管における苦味物質の受容と分泌作用 (2009)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Organizer: 生理研研究会「上皮組織における細胞外環境感受機構」
  • Place of Presentation: 自然科学研究機構生理学研究所
• [Presentation] tastant, 6-n-propyl-2-thiouracil(6-PTU) evokes anion secretion through bitter taste receptors in the human and rat large intestine (2009)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
• [Presentation] Bitter taste receptor(T2R) ligand, 6-n-propyl-2-thiouracil(6-PTU)-evoked anion secretion in human and rat colon : interaction with COX-2 activity (2009)
  • Author(s): Kaji I, Karaki SI, Kuwahara A
  • Organizer: Neurogastroenterology and Motility 2009 Joint International Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] Mode of effects of propionate on the motility of circular smooth muscle in the terminal ileum (2009)
  • Author(s): Tazoe H, Karaki SI, Kuwahara A
  • Organizer: Neurogastroenterology and Motility 2009 Joint International Meeting
  • Place of Presentation: Chicago, USA
• [Book] 腸管上皮における管腔内化学物質受容体が腸管機能に与える影響 (2011)
  • Author(s): 加治いずみ、唐木晋一郎、桑原厚和
  • Publisher: シーエムシー出版
• [Book] 消化管の管腔内化学受容と消化管機能制御 (2011)
  • Author(s): 唐木晋一郎, 桑原厚和
  • Publisher: アニマル・メディア
• [Book] 消化管の管腔内化学受容と消化管機能制御.消化管の栄養・生理と腸内細菌(Hindgut Club Japan編) (2011)
  • Author(s): 唐木晋一郎, 桑原厚和
  • Total Pages: 328
  • Publisher: アニマル・メディア社
• [Book] 腸管上皮における管腔内化学物質受容体が腸管機能に与える影響Luminal chemosensing and colonic ion transport (2011)
  • Author(s): 加治いずみ, 唐木晋一郎, 桑原厚和
  • Total Pages: 60
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://physiology.u-shizuoka-ken.ac.jp/index-ja.htm
• [Remarks]
  • URL: http://physiology.u-shizuoka-ken.ac.jp/index-ja.htm