Analysis pathologic processes of FAP development using double humanized mice

• Project/Area Number: 21590361
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human genetics
• Research Institution: Kumamoto University
• Principal Investigator: RI Chuhoa 熊本大学, 生命資源研究・支援センター, 特定事業研究員 (80398239)
• Co-Investigator(Renkei-kenkyūsha): YAMAMURA Kenichi 熊本大学, 生命資源研究・支援センター, 教授 (90115197)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 分子遺伝学 / レチノール結合タンパク(RBP) / トランスサイレチン(TTR) / ヒト化マウス / レチノール / レチノール結合タンパク / アミロイド / ES細胞 / 相同組換え / 置換アレル

Research Abstract

We successfully produced Rbp4 deficient mice, followed by the establishment of humanized RBP4 mice(Rbp4^<hRBP4>). These mice were mated with Ttr^<hTTR> mice to produce double humanized mice at both Ttr and Rbp4 loci. We obtained expected results from double humanized mice by molecular and biochemical analyses. These mice as optimum mouse model for familial amyloidotic polyneuropathy will be useful to analyze mechanisms for amyloid deposition and to devise a new way of treatment.

Research Products

• [Journal Article] Enhanced expression of human cDNA by phosphoglycerate kinase promoter-puromycin cassette in the mouse transthyretin locus (2011)
  • Author(s): Zhenghua Li, Gang Zhao, Jingling Shen, Kimi Araki, Kyoko Haruna, Seiya Inoue, Jun Wang, Ken-ichi Yamamura
  • Journal Title: Transgenic Research Volume: Vol.20 Pages: 191-200
  • URL: http://dx.doi.org/10.1007/s11248-010-9389-2
  • Peer Reviewed
• [Journal Article] Enhanced expression of human cDNA by phosphoglycerate kinase promoter-puromycin cassette in the mouse transthyretin locus (2011)
  • Author(s): Zhenghua Li, et al
  • Journal Title: Transgenic Research Volume: 20 Issue: 1 Pages: 191-200
  • DOI: 10.1007/s11248-010-9389-2
  • Peer Reviewed
• [Journal Article] Enhanced expression of human cDNA by phosphoglycerate kinase promoter-puromycin cassette in the mouse transthyretin locus. (2011)
  • Author(s): Li Z, et al.
  • Journal Title: Transgenic Research. Volume: 20 Pages: 191-200
  • Peer Reviewed
• [Journal Article] The transthyretin gene is expressed in Schwann cells of peripheral nerves (2010)
  • Author(s): Tatsufumi Murakami, Yutaka Ohsawa, Li Zhenghua, Ken-ichi Yamamura, Yoshihide Sunada
  • Journal Title: Brain research Volume: Vol.1248 Pages: 222-225
  • URL: http://dx.doi.org/10.1016/j.brainres.2010.06.017
  • Peer Reviewed
• [Journal Article] The transthyretin gene is expressed in Schwann cells of peripheral nerves (2010)
  • Author(s): Murakami T, et al.
  • Journal Title: Brain Research. Volume: 1348 Pages: 222-225
  • Peer Reviewed
• [Journal Article] Enhanced expression of human cDNA by phosphoglycerate kinase promoter-puromycin cassette in the mouse transthyretin locus (2010)
  • Author(s): Zhenghua Li, et al.
  • Journal Title: Transgenic Research (In press)
  • Peer Reviewed
• [Presentation] 可変型相同組換え法によるTtrおよびRbp遺伝子座のダブルヒト化マウスの作製 (2011)
  • Author(s): 李正花、荒木喜美、作村由美、山村研一
  • Organizer: 34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] 可変型相同組換え法によるTtrおよびRbp遺伝子座のダブルヒト化マウスの作製 (2011)
  • Author(s): 李正花, 等
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜,日本
  • Year and Date: 2011-12-13
• [Presentation] Production of optimum humanized mouse model for familial amyloidotic polyneuropathy (2011)
  • Author(s): Zhenghua Li, Kimi Araki, Ken-ichi Yamamura
  • Organizer: Mouse genetics 2011
  • Place of Presentation: ワシントンD. C.(米国)
  • Year and Date: 2011-06-23
• [Presentation] Production of Optimum Humanized Mouse Model for Familial Amyloidotic Polyneuropathy (2011)
  • Author(s): Zhenghua Li, et al
  • Organizer: Mouse genetics 2011
  • Place of Presentation: ワシントンD.C.,米国
  • Year and Date: 2011-06-23
• [Presentation] ヒト化マウスによる最適FAPモデルの作製 (2010)
  • Author(s): 李正花、荒木喜美、作村由美、山村研一
  • Organizer: 33回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] ヒト化マウスによる最適FAPモデルの作製 (2010)
  • Author(s): 李正花
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] Production of optimum humanized mouse model for familial amyloidotic polyneuropathy (2010)
  • Author(s): Zhenghua Li, Kimi Araki, Ken-ichi Yamamura
  • Organizer: The 4^<th> AFLAS Congress Meeting 5^<th> AMMRA Meeting & 11^<th> CSLAS Annual Meeting
  • Place of Presentation: 台北(台湾)
  • Year and Date: 2010-11-10
• [Presentation] Production of Optimum Humanized Mouse Model for Familial Amyloidotic Polyneuropathy (2010)
  • Author(s): Li Z, et al.
  • Organizer: The 4^<th> AFLAS Congress Meeting
  • Place of Presentation: Taipei Internationtl Convention Center TICC (Taipei, Taiwan)
  • Year and Date: 2010-11-10
• [Remarks]
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