A study of the effect of a recombinant immunosuppressive factor from parasites and immune-inhibitory mechanisms
| Project/Area Number |
21590464
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IRIKO Hideyuki 鳥取大学, 医学部, 助教 (60346674)
OTSUKI Hitoshi 鳥取大学, 医学部, 講師 (80403806)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 寄生虫 / マンソン裂頭条虫 / 免疫抑制因子 / マクロファージ / 旋毛虫 / 遺伝子発 / peroxiredoxin / 腹腔マクロファージ / alternative activation / 遺伝子組換えタンパク質 / cDNA / RACE法 / 遺伝子組み換えタンパク / 腹腔マクロフージ / プレロセルコイド / 遺伝子発現抑制 / クローニング |
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| Research Abstract |
An immunosuppressive factor(ES90) from Spirometra erinaceieuropaei plerocercoids was cloned and the recombinant ES90 was synthesized using wheat germ cell free expression system. However, the recombinant ES90 did not inhibit nitric oxide production in macrophages. Messenger RNA of Ym1, FIZZ1, and arginase 1 were expressed in peritoneal macrophages from Trichinella spiralis-infected mice. In connection with this, 2 types of peroxiredoxin(Prx) from T. spiralis were cloned, Prx-1 mRNA expression was stage-specific
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Report
(4 results)
Research Products
(2 results)
