Transcriptional Dysregulation in Abnormal Hematopoiesis and Leukemia
| Project/Area Number |
21591214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
OKUDA Tsukasa 京都府立医科大学, 医学研究科, 教授 (30291587)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKURA Chohei 京都府立医科大学, 医学研究科, 教授 (10285257)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血液腫瘍学 / 白血病 / 転写因子 / AML1 / RUNX / 造血制御 / 遺伝子改変マウス / 蛋白間相互作用 / 翻訳後修飾 / リン酸化 |
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| Research Abstract |
Transcription factor, AML1/Runx1, plays a pivotal role in hematopoietic development, and its dysfunction is closely related to leukemogenesis. In this study, the influence of leukemia-associated genomic alterations and post-translational modifications of this molecule were evaluated by use of biochemical analysis and experiments using mouse embryonic stem cells. In contrast to most cases of the leukemia-associated mutations that resulted in functional disruption, some mutants for modification residues of this molecule retained its biological activity. Mutant mouse lines for those mutations were currently being generated, and will be analyzed. A search for interacting molecules of AML1 was also performed as a supporting approach.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] A 5′untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model2010
Author(s)
Nagamachi A, Htun PW, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H
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Journal Title
Developmental Biology
Volume: 345
Pages: 226-236
Related Report
Peer Reviewed
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[Journal Article] RUNX3 promoter methylation in colorectal cancer : its relationship with microsatellite instability and its suitability as a novel serum tumor marker2010
Author(s)
Nishio M, Sakakura C, Nagata T, Komiyama S, Miyashita A, Hamada T, Kuryu Y, Ikoma H, Kubota T, Kimura A, Nakanishi M, Ichikawa D, Fujiwara H, Okamoto K, Ochiai T, Kokuba Y, Sonoyama T, Ida H, Ito K, Chiba T, Ito Y, Otsuji E
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Journal Title
Anticancer Research
Volume: 30
Pages: 2673-2682
Related Report
Peer Reviewed
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[Journal Article] Quantitative analysis of tumor-derived methylated RUNX3 sequences in the serum of gastric cancer patients2009
Author(s)
Sakakura C, Hamada T, Miyagawa K, Nishio M, Miyashita A, Nagata H, Ida H, Yazumi S, Otsuji E, Chiba T, Ito K, Ito Y
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Journal Title
Anticancer Research
Volume: 29
Pages: 2619-2625
Related Report
Peer Reviewed
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