Effect of rare sugars on ischemia-reperfusion injury of hepatectomized rats

• Project/Area Number: 21591751
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kagawa University
• Principal Investigator: HOSSAIN MOHAMMAD Akram 香川大学, 希少糖研究センター, 研究員 (20294754)
• Co-Investigator(Kenkyū-buntansha): TOKUDA Masaaki 香川大学, 医学部, 教授 (10163974)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Rare sugar D-psicose / Type 2 diabetes mellitus(T2DM) / OLETF rats / Insulin resistance / Adiposity / Pancreatic b-cells / D-allose / Rat liver / Ischemia reperfusion & resection / Mircocirculation / D-psicose / Type 2 diabetes / 双方〓〓 / oxidative stress / D-Allose / Rat / Liver / 90% resection / Regeneration

Research Abstract

Studies were undertaken to examine the effect of rare sugars D-allose and D-psicose.
Initially we performed the study on the effect of D-allose against ischemia-reperfusion injury of hepatectomized rats. 70% of rat livers were resected under 30 min total ischemia. Intravenous 100mg/ kg body weight D-allose was infused 30 min before inducing ischemia. Blood flow in the remnant liver was improved by D-allose till 60 min reperfusion. D-allose improved the remnant liver function and survival through the suppression of neutrophil enzyme MPO and stimulation of DNA labeling index and proliferating cell nuclear antigens(PCNA).
Next, we examined the effect of D-in lowering blood glucose and abdominal fat by using a T2DM model OLETF rats. Treated rats were fed with or without 5% D-psicose in drinking water for 13 weeks. D-psicose significantly reduced body weight and abdominal fat mass. Oral glucose tolerance test(OGTT) showed reduced blood glucose and insulin levels suggesting improvement of insulin resistance. Oil-red-O staining revealed reduced lipid accumulation in the liver. D-psicose also protected the pathological changes in pancreatic islets. These data demonstrate that D-psicose controls blood glucose levels by reducing lipotoxicity in liver and by preserving pancreatic b-cell function.

Research Products

• [Journal Article] Masaaki Tokuda Rare sugar D-psicose improves insulin sensitivity and glucose tolerance in type 2 diabetes Otsuka Long-Evans Tokushima Fatty(OLETF) rats (2011)
  • Author(s): Mohammad AHossain, Shigeru Kitagaki, Daisuke Nakano, Akira Nishiyama, Yasunobu Funamoto, Toru Matsunaga, Ikuko Tsukamoto, Fuminori Yamaguchi, Kazuyo Kamitori, Youyi Dong, Yuko Hirata, Koji Murao, Yukiyasu Toyoda
  • Journal Title: Biophysical and Biochemical research Communications Volume: 405 Pages: 7-12
• [Journal Article] Rare sugar D-psicose improves insulin sensitivity and glucose tolerance in type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats (2011)
  • Author(s): Mohammad A Hossain
  • Journal Title: Biophysical and Biochemical research Communications Volume: 405 Issue: 1 Pages: 7-12
  • DOI: 10.1016/j.bbrc.2010.12.091
  • Peer Reviewed
• [Presentation] Rare sugar D-psicose protects pancreas b-islets and thus improves insulin resistance in OLETF rats (2011)
  • Author(s): Mohammad A Hossain, Yuko Hirata, Kazuo Kamitori, Youyi Dong, Fuminori Yamaguchi, Masaaki Tokuda
  • Organizer: Japanese Society of Physiology ; Chugoku-Shikoku Regional Meeting
  • Place of Presentation: Hirashima University, Hiroshima, Japan(Oral)
• [Presentation] D-psicose protects pancreas□-islets and thus improves insulin resistance in OLETF Rats (2011)
  • Author(s): Mohammad A Hossain, Yuko Hirata, Kazuo Kamitori, Youyi Dong, Fuminori Yamaguchi, Masaaki Tokuda
  • Organizer: The 32nd Annual Meeting of Japan Society for the Study of Obesity
  • Place of Presentation: Awajishima, Japan(Poster)
• [Presentation] Rare Sugar D-Psicose Ameliorates Glucose Intolerance in Type-2 Diabetes OLETF Rat Model (2011)
  • Author(s): Mohammad A Hossain, Yuko Hirata, Kazuo Kamitori, Youyi Dong, Fuminori Yamaguchi, Masaaki Tokuda
  • Organizer: Federations of Asian and Oceanian Physiological Society Congress 2011
  • Place of Presentation: Taipei, Taiwan(Poster)
• [Presentation] Rare sugar D-psicose ameliorates glucose intolerance in type 2 diabetes rat model (2011)
  • Author(s): Mohammad Akram Hossain
  • Organizer: Federations of Asian and Oceanian Physiological Society Congress 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] D-psicose protects pancreas β-islets and thus improves insulin resistance in OLETF rats (2011)
  • Author(s): Mohammad Akram Hossain
  • Organizer: The 32nd Annual Meeting of Japan Society for the Study of Obesity
  • Place of Presentation: Awajishima, Japan
• [Presentation] Rare sugar D-psicose protects pancreas b-islets and thus improves insulin resistance in OLETF rats (2011)
  • Author(s): Mohammad Akram Hossain
  • Organizer: Japanese Society of Physiology ; Chugoku-Shikoku Regional Meeting
  • Place of Presentation: Hiroshima, Japan
• [Presentation] Rare sugar D-psicose protects pancreas b-islets and thus improves insulin resistance in OLETF rats (2011)
  • Author(s): Mohammad Akram Hossain
  • Organizer: The 5^<th> Symposium of International Society of Rare Sugars
  • Place of Presentation: Takamatsu, Japan
• [Presentation] Rare sugar D-psicose ameliorates glucose intolerance in type 2 diabetesrat model (2011)
  • Author(s): Mohammad Akram Hossain
  • Organizer: 3^<rd>.International Conference on Drug Discovery and Therapy
  • Place of Presentation: Dubai, UAE
• [Presentation] Ameliorative effect of a rare sugar D-psicose on glucose intolerance in type 2 diabetes rat model (2010)
  • Author(s): Mohammad Akram Hossain
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka, Iwate
• [Presentation] Effect of rare sugars on rat liver regeneration after 90% resection (2009)
  • Author(s): Mohammad Akram Hossain
  • Organizer: 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center, (Kyotofu)
  • Year and Date: 2009-07-27
• [Presentation] Rare sugar D-psicose protects pancreas b-islets and thus improves insulin resistance in OLETF rats
  • Author(s): Mohammad A Hossain, Yuko Hirata, Kazuo Kamitori, Youyi Dong, Fuminori Yamaguchi, Masaaki Tokuda
  • Organizer: The 5th Symposium of International Society of Rare Sugars
  • Place of Presentation: Takamatsu, Kagawa, Japan
• [Presentation] Rare sugar D-psicose ameliorates glucose intolerance in type-2 diabetes rat model
  • Author(s): Mohammad A Hossain, Kitagaki S, Nishiyama A, Toyoda Y, Yamaguchi F, Kamitori K, Dong Y, Hirata Y, Tokuda M
  • Organizer: 3^<rd>. International Conference on Drug Discovery and Therapy
  • Place of Presentation: Dubai, UAE(Poster)