Functional analysis of water channel for mild hypothermia-induced brain edema decrease using knockdown technique

• Project/Area Number: 21591982
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagoya City University
• Principal Investigator: FUJITA Yoshihito 名古屋市立大学, 大学院・医学研究科, 講師 (90238593)
• Co-Investigator(Kenkyū-buntansha): ASAI Kiyofumi 名古屋市立大学, 大学院・医学研究科, 教授 (70212462) ; SOBUE Kazuya 名古屋市立大学, 大学院・医学研究科, 教授 (90264738)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 水チャンネル / 脳浮腫 / ノックダウン / アクアポリン / 脳低温 / RANi / RNAi

Research Abstract

We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.We constructed vector of knockdown for AQP4, however, we could not obtain efficacy of knockdown for AQP4 using our constructed vectors. We tried a new method using lentiviral vectors

Research Products

• [Journal Article] 末梢静脈カテーテルからのAcinetobacter血流感染により敗血症性ショックに至った一例 (2012)
  • Author(s): 南仁哲、祖父江和哉、間渕則文、藤田義人
  • Journal Title: 日集中医誌 Volume: 19 Pages: 51-54
  • NAID: 10030288184
  • Peer Reviewed
• [Journal Article] Successful airway management in a patient with Goldenhar syndrome using preoperative three-dimensional computed tomography (2011)
  • Author(s): E Suzuki, H Hirate, Y Fujita, K Sobue
  • Journal Title: Anesth Intensive Care Volume: 39(4) Pages: 767-8
  • Peer Reviewed
• [Journal Article] 新型ドクターカー出動で救命した高濃度塩素ガス吸入による急性呼吸窮迫症候群(ARDS)の1症例 (2011)
  • Author(s): 大森隆夫、安井稔博、三宅健太郎、松本卓也、稲垣雅昭、成松紀子、山田富雄、山崎潤二、間渕則文、藤田義人
  • Journal Title: 日本救急医学中部地方会誌 Volume: 5 Pages: 19-22
  • Peer Reviewed
• [Journal Article] Before-after study of a restricted fluid infusion strategy for management of donor hepatectomy for living-donor liver transplantation (2010)
  • Author(s): Fujita Yoshihito, et al
  • Journal Title: J Anesthe 23 Pages: 67-74
  • NAID: 10025381639
  • Peer Reviewed
• [Journal Article] A novel skin-traction method is effective for real-time ultrasound-guided internal jugular vein catheterization in infants and neonates weighing less than 5 kilograms (2009)
  • Author(s): Morita M, Sasano H, Azami T, Sasano N, Fujita Y, Ito S, Sugiura T, Sobue K
  • Journal Title: Anesth Analg Volume: 109(3) Pages: 754-9
  • Peer Reviewed
• [Journal Article] Before-after study of a restricted fluid infusion strategy for management of donor hepatectomy for living donor liver transolantation (2009)
  • Author(s): Y Fujita, A Takeuchi, T Sugiura, T Hattori, Y Mizuochi, K Sobue
  • Journal Title: J. Anesth Volume: 23 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Continuous positive airway pressure applied through a bronchial blocker as a treatment for hypoxemia due to stenosis of the left main bronchus (2009)
  • Author(s): Sasano H, Sasano N, Ito S, Fujita Y, Sugiura T, Morita M, Sobue K
  • Journal Title: Anesthesiology Volume: 110(5) Pages: 1199-200
  • Peer Reviewed
• [Presentation] 予後予測因子としてのICU入室時血中乳酸値の検討 (2012)
  • Author(s): 藤田義人、他
  • Organizer: 第39回日本集中治療医学会学術集会
  • Place of Presentation: 幕張
• [Presentation] 予後予測因子としてのICU入室時血中乳酸値の検討 (2012)
  • Author(s): 藤田義人, ほか9名
  • Organizer: 日本集中治療医学会
  • Place of Presentation: 千葉県幕張メッセ
• [Presentation] 小児心臓術後患者における重症度評価としての乳酸値 (2011)
  • Author(s): 藤田義人、他
  • Organizer: 第19回日本集中治療医東海北陸地方会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-06-18
• [Presentation] 術前絶飲時間が1回拍出量変化量に及ぼす影響 (2011)
  • Author(s): 冨田麻衣子、藤田義人、他
  • Organizer: 第31回日本臨床麻酔学会
  • Place of Presentation: 沖縄
• [Presentation] 麻酔中の血中フェンタニル濃度の実測値と血中濃度シミュレーター算出値との比較 (2011)
  • Author(s): 藤掛数馬、藤田義人、他
  • Organizer: 第31回日本臨床麻酔学会
  • Place of Presentation: 沖縄
• [Presentation] 重症筋無力症患者に対する胸腺摘出術の麻酔管理における筋弛緩薬非使用の安全性 (2011)
  • Author(s): 青木智史、藤田義人、他
  • Organizer: 第31回日本臨床麻酔学会
  • Place of Presentation: 沖縄
• [Presentation] Cystatin C is a Predictor for the Length of ICU after Cardiac Surgery in Pediatric Patient (2011)
  • Author(s): S Yoshizawa, H Komura, Y Fujita, S Ito, K Sobue
  • Organizer: The American Society of Anesthesiologists Annual Meeting
  • Place of Presentation: Chicago Illinois, America
• [Presentation] Titration of intraoperative fentanyl in pediatric anesthesia (2011)
  • Author(s): S Yoshizawa, Y Fujita, H Sasano, T Sugiura, K Sobue
  • Organizer: 85^<th> International Anesthesia Research Society
  • Place of Presentation: Vancouver, British Columbia, Canada
• [Presentation] 小児麻酔におけるフェンタニル量の滴定 (2010)
  • Author(s): 藤田義人、他
  • Organizer: 第57回日本麻酔学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-06-03
• [Presentation] 重回帰分析を用いた来院時心肺停止症例の予後予測 (2010)
  • Author(s): 藤田義人、他
  • Organizer: 第38回日本救急医学会
  • Place of Presentation: 東京